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Marine bacillus licheniformis protein and application thereof to cardiovascular field

A technology of Bacillus licheniformis and Bacillus, applied in the field of medicine, can solve the problem of underutilization of microorganisms, and achieve the effect of low cost and simple process

Inactive Publication Date: 2014-08-06
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Kelp also contains some associated microorganisms, but these microorganisms and their metabolites have not been fully utilized so far

Method used

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Embodiment Construction

[0014] The embodiments of the present invention proceed in sequence as follows:

[0015] a. Inoculate the strain Bacillus licheniformis DNN6 in a liquid medium with sucrose 40-50 g / L and peptone 8-10 g / L, pH 9, 100-200 mL liquid medium per bottle, temperature 30-35℃ , The rotation speed is 150-200 rpm, the shaker culture is 4-5 days. The Bacillus licheniformis strain was deposited at the General Microbiology Center (CGMCC) of the China Microbial Culture Collection Management Committee (CGMCC), located at No. 3, Beichen West Road, Chaoyang District, Beijing, on December 31, 2012. The deposit number: CGMCC No: 7077 .

[0016] b. Centrifuge the fermentation broth at 8000 r / min to remove the bacteria, and then add ammonium sulfate. The final saturation concentration of ammonium sulfate is 95%. Let stand overnight at 4°C. After filtering the precipitate to dissolve it with distilled water, the molecular weight cutoff is 3600. The dialysis bag of Dalton was dialyzed for 4 days, and the...

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PUM

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Abstract

A marine bacillus licheniformis protein. A preparation method comprises the following steps: inoculating a kelp endophytic bacillus licheniformis DNN6 strain to a liquid medium, wherein the liquid medium formula comprises 40-50g / L sucrose and 8-10g / L peptone, and has pH value adjusted to 9; bottling 150-250mL of the liquid medium into each bottle, and conducting shaking culture at temperature of 30-35 DEG C, rotating speed of 150-250 rpm for 4-5 days; centrifuging the fermentation liquid at 8000 r / min to remove thalli, then adding ammonium sulfate, wherein the saturation concentration of ammonium sulfate is 95%, and standing overnight at 4 DEG C; and filtering, dissolving the precipitate with distilled water, then conducting dialysis by using a dialysis bag with cut-off molecular weight of 3600 Dalton for 4 days, and conducting freeze-drying on the products in the dialysis bag, so as to obtain the marine endophytic bacillus licheniformis protein. The invention also provides the application of the protein to the cardiovascular field. The process is simple and low in cost.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a kelp endophytic Bacillus licheniformis DNN6 ( Bacillus licheniformis DNN6) strain protein and its application in cardiovascular. Background technique [0002] kelp( Laminaria Japonica Aresch) is a large marine brown algae plant that grows in low-temperature seawater. It grows in the sea where the water temperature is low. Kelp mainly grows naturally, and is also cultivated artificially. Most of them are sold in the market as dry products. The quality is brown, short, thin and thick. Kelp has the reputation of "longevity vegetable", "sea vegetable" and "champion of iodine content". [0003] As an important marine resource, kelp has long been known for its edible and medicinal value. The active ingredients extracted from kelp have the functions of lowering blood sugar, lowering blood fat, anti-tumor, anti-HIV and enhancing immune function, and have certain effects on the treatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/32C07K1/30A61P9/00A61P9/12
CPCC07K14/32A61K38/00
Inventor 张付云徐凤杨阳王维才
Owner DALIAN OCEAN UNIV
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