Tumor living tissue in-vitro culture system and culture method

Abstract

The invention relates to a tumor living tissue in-vitro culture system and a corresponding tumor living tissue in-vitro culture method. The tumor living tissue in-vitro culture system comprises a support system for simulating an in-vivo basilemma structure and a blood supply system of tumor, a tumor tissue specimen treatment liquid which is a RPMI1640 culture liquid containing 2mu g / ml of amphotericin, 1000U / ml of penicillin and 1000mu g / ml of streptomycin, and a special culture medium for tumor tissue culture, wherein the culture medium contains 20mu g / L of bFGF (Basic Fibroblast Growth Factor), 20mu g / L of EGF (Epidermal Growth Factor), 10mu g / L of PDGF (Platelet Derived Growth Factor), 10mu g / L of HGF (Hepatocyte Growth Factor), 10mu g / L of IGF (Insulin-like Growth Factor), 10mu g / L of NRG1, 10mu g / L of VEGF (Vascular Endothelial Growth Factor), 5% of fetal calf serum as well as 500U / ml of penicillin and 500mu g / ml of streptomycin on the basis of a mixture solution containing 50 percent of DMEM (Dulbecco Modified Eagle Medium) and 50 percent of HamF 12. The invention provides a systematic solution scheme and an appropriate culture medium for the in-vitro culture of tumor tissue cells.

Description

technical field [0001] The invention relates to an in vitro culture system of tumor living tissue, in particular to a special in vitro culture solution for tumor living tissue, and also to a corresponding in vitro culture method for tumor living tissue. Background technique [0002] Studies have shown that different types of tumors have different biological characteristics; the same type of tumors have different biological characteristics in different individuals. In order to study the biological characteristics of individual tumor tissue cells, such as the sensitivity of tumor tissue cells to radiotherapy and chemotherapy, and provide evidence for the diagnosis and treatment of tumor patients, it is necessary to culture primary tumor tissue cells. [0003] Simulate the microenvironment in vivo [0004] First of all, the tumor cells leaving the human body often undergo anoikis due to changes in the way of nutrient absorption and the microenvironment in which they survive. ...

AI Technical Summary

Problems solved by technology

[0013] Because the in vitro culture of tumor tissue cells is affected by many of the above factors, there is currently no systematic solution to the culture of tumor tissue cells and a suitable medium at home and abroad, and there is no commercially available medium and culture technology for tumor tissue culture. Therefore, it is necessary to develop an effective tumor living tissue in vitro culture system and corresponding culture methods.

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