Method for detecting metallothioneins in aquatic organism

Abstract

The invention discloses a method for detecting metallothioneins in an aquatic organism, and belongs to the technical field of electrochemistry. The principle of the detecting method is as follows: metallothionein molecules to be detected in a solution gather onto the surface of a mercury electrode of the selected gathering potential to react with supporting electrolyte Pt(NH3)2Cl2 to generate H+; the reacted H+ is reduced when the potential scans about -1.48 V, so that characteristic peaks and corresponding currents are generated in a voltammogram. As the method is high in sensitivity and low in detection limit, the quantity of required samples is less; as reaction is controlled by diffusion, the action is less impacted by competitive adsorption, that is, the detection is interfered little by other proteins in the sample to be analyzed, so that a standard curve method is suitable for detecting. The method provided by the invention has the advantages of being simple, high in reliability and accuracy, and low in detection limit.

Description

technical field [0001] The invention discloses a method for detecting metallothionein in aquatic organisms, and in particular relates to an electrochemical method for qualitatively and quantitatively analyzing metallothionein in aquatic organisms. Background technique [0002] Metallothionein (MT) is a low-molecular-weight specific protein that can be induced by metals. It exists widely in organisms, is rich in cysteine, does not contain aromatic amino acids, and rarely contains histamine. Acids play a role in detoxifying heavy metals, storing and transporting essential elements, scavenging free radicals, and regulating gene expression in organism tissues. [0003] In the study of aquatic environment ecology, the role of metallothionein is especially valued by people. This is because a remarkable feature of metallothionein is that the transcription of its gene can be induced by environmental metals, and the transcription level is directly related to the concentration of env...

AI Technical Summary

Problems solved by technology

The sensitivity of the metal saturation method is not high, and it is easily interfered by other molecules that can bind metals, especially in aquatic organisms, it is easy to accumulate substances in the environment, so it cannot accurately and quantitatively analyze the content of metallothionein in organisms, and the specificity is poor

Spectrophotometry is based on the reaction of sulfhydryl groups in metallothionein. Therefore, in order to minimize the interference caused by small molecules containing sulfhydryl groups, the treatment of crude metallothionein extracts is more complicated.

The most widely used electrochemical method for the detection of metallothionein in aquatic organisms is differential pulse polarography (DPP). The output limit is high, and the literature shows that this method cannot accurately reflect the metallothionein in crustaceans [Pedersen KL, Pedersen S N, Knudsen J, et al. Quantification of metallothionein by differential pulsepolarography overestimates concentrations in crustaceans[J].Environmental science&technology,2008,42(22):8426-8432]

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