Identification method for fingerprint spectrum of salvia miltiorrhiza medicinal material

A technology of fingerprint and Salvia miltiorrhiza, applied in the identification field of Salvia miltiorrhiza medicinal materials, can solve the problems of few components, poor reproducibility of the same sample, few chemical components, etc., and achieve a good separation effect.

Active Publication Date: 2014-08-06
XIAN SHIJISHENGKANG PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The 2010 edition of "Chinese Pharmacopoeia" uses thin-layer chromatography to identify Danshen. Since the thin-layer identification method itself is affected by many factors such as temperature, humidity, thickness of the thin-layer plate, and sample loading, the chemical composition of the sample is not fully reflected. The number of identifiable components is small and the reproducibility of the same sample is not good
However, the Danshen fingerprint technology reported in the literature has fewer chemical components and provides a small amount of information, which cannot well represent the overall quality of Danshen medicinal materials and cannot effectively identify the quality of Danshen medicinal materials.

Method used

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  • Identification method for fingerprint spectrum of salvia miltiorrhiza medicinal material
  • Identification method for fingerprint spectrum of salvia miltiorrhiza medicinal material
  • Identification method for fingerprint spectrum of salvia miltiorrhiza medicinal material

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Determination of Fingerprint Spectrum of Salvia Miltiorrhiza

[0034]1. Instruments: Agilent 1260 high performance liquid chromatography, G1311C quaternary pump, column thermostat, G1316A, G1315D, DAD detector, Agilent Zorbax Eclipse XDB C18 column (4.6×250mm, 5μm). The mass spectrometer system is an ESI-MSn mass spectrometer (Bruker).

[0035] [0025 Reagents: Acetonitrile was chromatographically pure (Burdick&Jackson, Honeywell International Inc., USA), glacial acetic acid was chromatographically pure (Tedia company Inc., USA), water (Millipore, Bedford, MA, USA), and other reagents were analytically pure (Sinopharm Group Chemical Reagents Ltd). Danshensu, protocatechualdehyde and salvianolic acid B reference substances were purchased from China Biological Products Co., Ltd. Salvianolic acid A, salvianolic acid E, and salvianolic acid D are self-made in this laboratory. All samples are reserved in the Sample Room of the Modernization Center of Traditi...

Embodiment 2

[0059] Embodiment 2: Determination of Fingerprint Spectrum of Salvia Miltiorrhiza

[0060] 1. Instruments: Agilent 1260 high performance liquid chromatography, G1311C quaternary pump, column thermostat, G1316A, G1315D, DAD detector, Agilent Zorbax Eclipse XDB C18 column (4.6×250mm, 5μm). The mass spectrometer system is an ESI-MSn mass spectrometer (Bruker).

[0061] Reagents: Acetonitrile was chromatographically pure (Burdick&Jackson, Honeywell International Inc., USA), glacial acetic acid was chromatographically pure (Tedia company Inc., USA), water (Millipore, Bedford, MA, USA), and other reagents were analytically pure (Sinopharm Group Chemical Reagents Ltd). Danshensu, protocatechualdehyde and salvianolic acid B reference substances were purchased from China Biological Products Co., Ltd. Salvianolic acid A, salvianolic acid E, and salvianolic acid D are self-made in this laboratory. All samples are reserved in the Sample Room of the Modernization Center of Traditional...

Embodiment 3

[0071] Embodiment 3: Determination of Fingerprint Spectrum of Salvia Miltiorrhiza

[0072] 1. Instruments: Agilent 1260 high performance liquid chromatography, G1311C quaternary pump, column thermostat, G1316A, G1315D, DAD detector, Agilent Zorbax Eclipse XDB C18 column (4.6×250mm, 5μm). The mass spectrometer system is an ESI-MSn mass spectrometer (Bruker).

[0073] Reagents: Acetonitrile was chromatographically pure (Burdick&Jackson, Honeywell International Inc., USA), glacial acetic acid was chromatographically pure (Tedia company Inc., USA), water (Millipore, Bedford, MA, USA), and other reagents were analytically pure (Sinopharm Group Chemical Reagents Ltd). Danshensu, protocatechualdehyde and salvianolic acid B reference substances were purchased from China Biological Products Co., Ltd. Salvianolic acid A, salvianolic acid E, and salvianolic acid D are self-made in this laboratory. All samples are reserved in the Sample Room of the Modernization Center of Traditional...

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Abstract

The invention provides an identification method for a fingerprint spectrum of a salvia miltiorrhiza medicinal material. The method consists of: preparation of a test solution, measurement of a fingerprint spectrum, determination of a control fingerprint spectrum, and identification of a salvia miltiorrhiza medicinal material fingerprint spectrum. By high performance liquid chromatography (HPLC) fingerprint spectrum measurement and analysis on the salvia miltiorrhiza medicinal material, 5 characteristic peaks of the salvia miltiorrhiza medicinal material are determined, and the common characteristic peaks constitute fingerprint features of the salvia miltiorrhiza medicinal material and can serve as the control fingerprint spectrum of the salvia miltiorrhiza medicinal material. The salvia miltiorrhiza medicinal material needing identification can be compared with the control fingerprint spectrum to inspect the situation of the common characteristic peaks so as to identify quality. With the characteristics of simplicity, good repeatability, obvious characteristic peaks, accuracy and reliability, etc., the method provided by the invention is conducive to identifying the quality of the salvia miltiorrhiza medicinal material.

Description

technical field [0001] The invention relates to a method for identifying the fingerprints of Danshen medicinal materials, in particular to a method for identifying the quality of effective components of Danshen medicinal materials by using the high-performance liquid chromatography fingerprint method. Background technique [0002] Salvia miltiorrhiza has the effects of activating blood and regulating menstruation, dispelling blood stasis and relieving pain, cooling blood and eliminating carbuncle, clearing heart and eliminating troubles, nourishing blood and calming nerves. Salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge. Danshen mainly contains crystalline phenanthrene quinone compounds: tanshinone I, IIA, IIB, isotanshinone I, isotanshinone II, cryptotanshinone, isocryptanshinone, hydroxytanshinone IIA, tanshinone, L-dihydrotanshinone I, tanshinol, etc. In addition, it still contains protocatechuic aldehyde, protocatechuic acid, succinic acid, u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 果德安吴婉莹赵丙贤吕延英刘蓉霞姚帅王俊平赵兴蔡录影王丹丹侯晋军笪娟
Owner XIAN SHIJISHENGKANG PHARMA IND
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