ELISA (Enzyme-linked Immunoassay Assay) plate and kit for predicting ALI (Acute Lung Injury)/ARDS (Acute Respiratory Distress Syndrome) and assessing prognosis of ALI/ARDS

An enzyme-linked immunoassay detection and prognosis technology, applied in measurement devices, instruments, disease diagnosis, etc., can solve the problem of not predicting the survival of ALI/ARDS, save manpower and time, improve work efficiency, and achieve accurate results.

Active Publication Date: 2014-08-06
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no clinical kit for predicting ALI / ARDS and estimating its prognosis to predict the progression of the disease and assess survival

Method used

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  • ELISA (Enzyme-linked Immunoassay Assay) plate and kit for predicting ALI (Acute Lung Injury)/ARDS (Acute Respiratory Distress Syndrome) and assessing prognosis of ALI/ARDS
  • ELISA (Enzyme-linked Immunoassay Assay) plate and kit for predicting ALI (Acute Lung Injury)/ARDS (Acute Respiratory Distress Syndrome) and assessing prognosis of ALI/ARDS
  • ELISA (Enzyme-linked Immunoassay Assay) plate and kit for predicting ALI (Acute Lung Injury)/ARDS (Acute Respiratory Distress Syndrome) and assessing prognosis of ALI/ARDS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An enzyme-linked immunoassay panel for predicting ALI / ARDS and estimating its prognosis, comprising a solid phase carrier, on which a monoclonal antibody of bone morphogenic protein 15 (BMP-15), CXC chemotactic Monoclonal antibody to factor ligand 16 (CXCL16), monoclonal antibody to CXC chemokine receptor 3 (CXCR3), monoclonal antibody to interleukin-6 (IL-6), Wilms overexpressed gene (CCN3 / NOV ), monoclonal antibody to insulin-like growth factor binding protein 4 (IGFBP-4), monoclonal antibody to interleukin-5 (IL-5), monoclonal antibody to interleukin 5 receptor (IL-5R alpha) , monoclonal antibody to interleukin 22 receptor binding protein antibody (IL-22BP), monoclonal antibody to leptin (Leptin / OB), monoclonal antibody to macrophage inflammatory protein-1D (MIP-1d), hormone Monoclonal antibody to B (Orexin B), monoclonal antibody to CC class chemokine receptor 2 (CCR2), monoclonal antibody to transforming growth factor-beta5 (TGF-beta5), and blank control wells. Th...

Embodiment 2

[0035] Collect 10 cases of normal human serum and 10 cases of ALI / ARDS patient serum as the normal group and ALI / ARDS group, use the kit in Example 1, and detect according to the detection steps in Example 1, the combined expression of 14 factors changes As shown in Table 1:

[0036] Table 1: Expression changes of 14 cytokines in ALI / ARDS patients and normal human serum

[0037]

[0038] As shown in Table 1, the OD ratios of the above 14 cytokines between the two groups were all higher than 3, and there were statistical differences in the OD values ​​between the ALI / ARDS group and the normal group. The above results indicated that the 14 cytokines were significantly elevated in the serum of ALI / ARDS patients, so the simultaneous detection of the above 14 cytokines can be used as a clinical auxiliary means for the diagnosis and poor prognosis of ALI / ARDS.

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Abstract

The invention provides an ELISA (Enzyme-linked Immunoassay Assay) plate and kit for predicting ALI (Acute Lung Injury)/ARDS (Acute Respiratory Distress Syndrome) and assessing prognosis of the ALI/ARDS. The ELISA plate comprises a solid phase carrier which is provided with a monoclonal antibody of bone morphogenetic protein 15, a monoclonal antibody of CXC chemokine ligand 16, a monoclonal antibody of CXC chemokine receptor 3, a monoclonal antibody of interleukin-6, a monoclonal antibody of nephroblastoma overexpression genes, a monoclonal antibody of insulin-like growth factor binding protein 4, a monoclonal antibody of interleukin-5, a monoclonal antibody of interleukin 5 receptor, a monoclonal antibody of interleukin 22 receptor binding protein, a monoclonal antibody of leptin, a monoclonal antibody of macrophage inflammatory protein-1D, a monoclonal antibody of orexin B, a monoclonal antibody of CC type chemokine receptor 2, a monoclonal antibody of transforming growth factor-beta5 and blank control holes. The ELISA plate and kit can achieve accurate results and are simple to operate.

Description

technical field [0001] The invention relates to a kit for in vitro diagnosis, in particular to a kit for predicting the prognosis of acute lung injury / acute respiratory distress syndrome by detecting the combined changes of cytokine content in blood by enzyme-linked immunoassay. Background technique [0002] Acute lung injury (Acute Lung Injury, ALI) was first proposed in 1967. It is the injury of alveolar epithelial cells and capillary endothelial cells caused by various direct and indirect injury factors, resulting in diffuse pulmonary interstitial and alveolar edema, resulting in acute lung injury. Hypoxic respiratory insufficiency. The pathophysiological features are reduced lung volume, decreased lung compliance, and imbalance of ventilation / perfusion flow. The clinical manifestations are sudden progressive hypoxemia and respiratory distress, and the lung imaging shows heterogeneous exudative lesions. . The development of ALI to a severe stage (oxygenation index <2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/544
CPCG01N33/545G01N33/6884G01N2800/125G01N2800/50
Inventor 陈成水王向东石林李玉苹陈超蕾沈伟
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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