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Method for extracting erianin from dendrobe

A technology of Erianin and Dendrobium, which is applied in ether separation/purification, ether preparation, organic chemistry, etc., can solve the problems of large amount of solvent for extraction of silica gel column chromatography, unsuitable for industrialized large-scale production, and many impurities in the extraction target. , to achieve the effects of low elution solvent cost, large sample loading and low preparation cost

Active Publication Date: 2014-08-13
云南金九地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These extraction methods have high equipment investment costs, too many impurities in the extraction target, chloroform extraction method Erianin extract content can only reach 1-10%, large amount of extraction solvent for silica gel column chromatography, long period, and low reusability of fillers. Suitable for industrial mass production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the method for extracting erianin from Dendrobium dendrobii, concrete operations are as follows:

[0022] (1) Take 1 kg of dried Dendrobium drumstick produced in Dehong, Yunnan and grind it to a particle size of 60 mesh;

[0023] (2) Dendrobium powder was reflux extracted three times with a methanol solution of pH=10 (prepared by adjusting the pH of a methanol solution with a mass percentage concentration of 60% to 10 with sodium bicarbonate) at an extraction temperature of 80°C, of ​​which 5L of methanol was used for the first time The solution was refluxed for 2 hours and filtered, the filter residue was refluxed for the second time with 4L methanol solution for 1.5 hours and then filtered, the filter residue was refluxed for the third time with 4L methanol solution for 0.5 hours and filtered, and the three extractions were combined; methanol was recovered to obtain a black-red crude extraction paste 113 grams;

[0024] (3) Use 250ml of acetonitrile to ...

Embodiment 2

[0029] Embodiment 2: the method for extracting erianin from Dendrobium dendrobium, the specific operations are as follows:

[0030] (1) Take 1 kg of dried Dendrobium candidum from Guangnan, Yunnan and crush it to 50 mesh;

[0031] (2) Dendrobium powder was reflux extracted three times with a methanol solution of pH=9 (the pH of a methanol solution with a mass percent concentration of 70% was adjusted to 9 with sodium hydroxide), and the extraction temperature was 75°C. The first time 5L of methanol The solution was refluxed and extracted for 2.5 hours and then filtered, the filter residue was reflux extracted with 4L methanol solution for 2 hours and then filtered, the filter residue was reflux extracted with 4L methanol solution for 0.6 hours and then filtered, and the three extracts were combined; methanol was recovered to obtain 71 grams of black and red crude extract paste ;

[0032] (3) The crude extract was ultrasonically dissolved in 250 ml of acetonitrile, filtered, and...

Embodiment 3

[0037] Embodiment 3: the method for extracting erianin from Dendrobium dendrobium, the specific operations are as follows:

[0038] (1) Take 1 kg of dried Dendrobium fringe from Guangnan, Yunnan and crush it to 40 mesh;

[0039] (2) Dendrobium powder was refluxed three times with methanol solution of PH = 8 (made by adjusting the pH of the methanol solution with a concentration of 50% by mass percentage to 8 with ammonia water) at an extraction temperature of 78°C, and the first 5L of methanol solution was refluxed After extracting and extracting for 3 hours, filter, filter the filter residue with 4L methanol solution for reflux extraction for 1.5 hours, then filter, filter the filter residue for 1 hour with 4L methanol solution for reflux extraction, and combine three extracts; recover methanol to obtain 150 grams of black-red crude extraction paste;

[0040] (3) The crude extract was ultrasonically dissolved in 250 ml of acetonitrile, filtered, and the insoluble residue was ...

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Abstract

The invention discloses a method for extracting erianin from dendrobe. The method comprises the following steps: extracting by using an alkaline organic solvent, mixing and extracting by a nonaqueous phase organic solvent, performing small-pore diameter resin separation, crystallizing by organic solvents, and preparing to obtain high-purity erianin square crystal. Compared with a conventional process method, the method has the advantages that the process is simple, the crude extraction solvent is low in cost, the target is fewer in non-aqueous phase extraction impurities, the column chromatography cost is low, the packing is high in separation precision and can be repeatedly utilized, the chromatography solvent cost is low, the environmental pollution is relatively low, the erianin is high in yield, the purity is high and the like. Moreover, the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a high-efficiency and low-cost method for extracting Erianin from dendrobium, belonging to the field of extracting plant active substances. Background technique [0002] Dendrobium Dendrobium Sw. It is one of the largest genus of Orchidaceae, with more than 1500 species of plants, widely distributed in tropical and subtropical regions such as Asia, Europe and Oceania. There are about 80 species in our country, mainly distributed in southwest, east and south China. Dendrobium plants are epiphytes with a unique growth environment and strict requirements on the microclimate environment. It mostly grows on cool and high-humidity shady slopes and semi-shady slopes on slightly acidic rock cliffs. It is distributed in clusters, with trees shading above and streams and ditches below. It is slightly drought-resistant in winter and spring, but leaves often fall when it is severely lacking in water. The bare stem survives the adverse e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C43/23C07C41/34C07C41/36C07C41/38
CPCC07C41/34C07C41/36C07C43/23
Inventor 蔡群虎田方
Owner 云南金九地生物科技有限公司
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