Optimized high-yield technique for producing L-arginine Corynebacterium crenatum by batch fermentation

Abstract

The laboratory systemically researches the induced mutation breeding and strain metabolism engineering modification for producing L-arginine by Corynebacterium crenatum fermentation, and 4 national patents of invention have been authorized to related achievements. On the basis of early research, the invention provides an optimized high-yield technique for producing L-arginine Corynebacterium crenatum by batch fermentation, which is an improvement on the basis of the previous technique for producing L-arginine by Corynebacterium crenatum fermentation; and the improvement of the seed culture and fermentation technique is mainly utilized to enhance the L-arginine yield. In the seed culture process, the seed growth speed is high, and the final bacterium concentration is high. In the fermentation process, the fermentation culture medium is simple in components and does not need vitamins, amino acids or other minor elements, the technique is easy to control, the fermenting property of the strain is stable, and the L-arginine yield is up to 56.3g / L and is one of the maximum yield in China at present; and the sugar acid conversion rate is up to 32%, and is the highest level in China at present.

Description

technical field [0001] The invention belongs to the technical field of amino acid production by microbial fermentation, and in particular relates to a fermentation process for improving L-arginine fermentation yield and glucose utilization rate. technical background [0002] L-Arginine is a semi-essential amino acid in humans and animals, and it is a precursor for the synthesis of various biologically active substances, and has a variety of unique physiological and pharmacological effects. With the continuous in-depth research and understanding of the biological functions of arginine, arginine has become more and more widely used in medicine, food, and feed industries. [0003] The production methods of L-arginine include hydrolysis and fermentation. At present, most of the existing manufacturers in my country still mainly use protein hydrolysis to produce L-arginine. This method has serious environmental pollution and low yield, and is not suitable for large-scale producti...

AI Technical Summary

Problems solved by technology

However, in domestic production of L-arginine by microbial fermentation, the level of acid production is generally low, the cost is high, and the production level and output cannot meet domestic demand.