Method for identifying key toxic substances in pesticide wastewater by taking daphnia magna toxicity as guide

Abstract

The invention discloses a method for identifying key toxic substances in pesticide wastewater by taking daphnia magna toxicity as a precursor and belongs to the field of identification of non-target pollutants. The method comprises the following steps: performing enrichment on organic water sample extract; performing a daphnia magna toxicity experiment on an organic water sample extract; performing fractional separation on the organic water sample extract, and separating compounds of different polarities; performing the daphnia magna toxicity experiment on the separated components; and performing qualitative identification and quantitative determination on the compounds in toxic substances. The compounds of different polarities are separated by utilizing a fractional separation technology, the toxic substances are screened to reduce the complexity of a sample, the potential toxic substances are qualitatively identified through a high performance liquid chromatography-time-of-flight mass spectrometry, suspicious toxic substances are quantitatively determined by utilizing a liquid chromatography mass spectrometry, and the defect that key toxic pollutants in a mixing system cannot be obtained by the traditional non-target chemical analysis is overcome. Meanwhile, due to fractional separation and feature object screening, the method is high in resolution ratio, high in accuracy and high in information amount, so that the identification of non-target key toxic substances is relatively convenient and reliable.

Description

technical field [0001] The invention relates to a method for identifying the toxicity of pesticide wastewater with different pollution levels and the identification of toxic organic substances, specifically a method for identifying key toxic substances in pesticide wastewater with the toxicity of Daphnia magna as the guide, based on the acute toxicity of Daphnia magna and the identification of toxic organic substances. Methods for screening, qualitative identification and quantitative confirmation of toxic substances for fractionation of mixed contaminated samples. Background technique [0002] Pesticides are used to kill insects, fungi, and other organisms that harm crop growth. Due to its high efficiency, China has used it in large quantities and has become a big country in the production and use of pesticides. "Monitoring and Analysis of China's Pesticide Market Output in 2012" shows that my country produced 3.549 million tons of pesticides in 2012, ranking first in the ...

AI Technical Summary

Problems solved by technology

However, due to the wide variety of organic substances, the specific category of organic substances cannot be determined when using the TIE method to identify toxicants

[0004] Through searching, it is found that the domestic and foreign literature has not reported the acute toxicity of pesticide wastewater to Daphnia magna. The toxic components were separated by preparative chromatography, and the quadrupole-time-of-flight mass spectrometry (Quadrupole Time Of Flight, Q-TOF) and quantitative Ecological Structure Activity Relationships (QSAR) ECOSAR (Ecological Structure Activity Relationships) software estimates the toxicity of compounds to track key toxicants in pesticide wastewater

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