Method for identifying key toxic substances in pesticide wastewater by taking daphnia magna toxicity as guide

A technology for pesticide wastewater and large fleas, which is applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as inability to determine the type of organic matter, and achieve the effect of reducing sample complexity, reducing sample complexity, and high resolution.

Active Publication Date: 2014-08-13
NANJING UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the wide variety of organic substances, the specific category of organic substances cannot be determined when using the TIE method to identify toxicants
[0004] Through searching, it is found that the domestic and foreign literature has not reported the acute toxicity of pesticide wastewater to Daphnia magna. The toxic components

Method used

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  • Method for identifying key toxic substances in pesticide wastewater by taking daphnia magna toxicity as guide
  • Method for identifying key toxic substances in pesticide wastewater by taking daphnia magna toxicity as guide
  • Method for identifying key toxic substances in pesticide wastewater by taking daphnia magna toxicity as guide

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Establishment of preparative chromatographic separation technology:

[0042] (1) Establishment of the efflux curves of organic nitrogen pesticides (Benzamid, Metrizone, Hexazinone, Triadimefon, DEET), polycyclic aromatic hydrocarbons, nonylphenol, and octylphenol: , mecitrione, hexazinone, triadimefon, DEET), polycyclic aromatic hydrocarbons, nonylphenol, octylphenol standard samples were separated by preparative chromatography and the separated components were concentrated. Quantitative analysis of polycyclic aromatic hydrocarbons, nonylphenol and octylphenol in the separated components was carried out by high performance liquid chromatography. Quantitative analysis of organic nitrogen pesticides in the separated components by high performance liquid chromatography-mass spectrometry. Finally, the distribution of the concentration and efflux time of the compound was obtained, and the efflux of organic nitrogen pesticides (bizamid, metrizone, hexazinone, triadimefon, DE...

Embodiment 2

[0046] A method for identifying key toxic substances in pesticide wastewater based on daphnia magna toxicity, the steps of which are: (1) solid phase extraction: the influent of pesticide wastewater treatment plants is filtered through a glass filter with a 0.45um nylon membrane, and the amount The filtered 4L water sample was enriched by Waters Oasis HLB. The column was activated with n-hexane, dichloromethane, methanol and deionized water before use, and the flow rate was controlled at 1-2 Drops per second, each column enriches 500mL of water sample; after the extraction, centrifuge at 5000r / min for 5min to remove the water in the extraction column.

[0047] (2) Enrichment: Use 5mL of n-hexane, 10mL of dichloromethane, and 10mL of methanol to elute the influent organic matter from the solid-phase extraction column and collect the eluate, spin the eluent to evaporate, blow with nitrogen to concentrate, and replace the solvent Then dilute to 1mL and transfer to the injection v...

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Abstract

The invention discloses a method for identifying key toxic substances in pesticide wastewater by taking daphnia magna toxicity as a precursor and belongs to the field of identification of non-target pollutants. The method comprises the following steps: performing enrichment on organic water sample extract; performing a daphnia magna toxicity experiment on an organic water sample extract; performing fractional separation on the organic water sample extract, and separating compounds of different polarities; performing the daphnia magna toxicity experiment on the separated components; and performing qualitative identification and quantitative determination on the compounds in toxic substances. The compounds of different polarities are separated by utilizing a fractional separation technology, the toxic substances are screened to reduce the complexity of a sample, the potential toxic substances are qualitatively identified through a high performance liquid chromatography-time-of-flight mass spectrometry, suspicious toxic substances are quantitatively determined by utilizing a liquid chromatography mass spectrometry, and the defect that key toxic pollutants in a mixing system cannot be obtained by the traditional non-target chemical analysis is overcome. Meanwhile, due to fractional separation and feature object screening, the method is high in resolution ratio, high in accuracy and high in information amount, so that the identification of non-target key toxic substances is relatively convenient and reliable.

Description

technical field [0001] The invention relates to a method for identifying the toxicity of pesticide wastewater with different pollution levels and the identification of toxic organic substances, specifically a method for identifying key toxic substances in pesticide wastewater with the toxicity of Daphnia magna as the guide, based on the acute toxicity of Daphnia magna and the identification of toxic organic substances. Methods for screening, qualitative identification and quantitative confirmation of toxic substances for fractionation of mixed contaminated samples. Background technique [0002] Pesticides are used to kill insects, fungi, and other organisms that harm crop growth. Due to its high efficiency, China has used it in large quantities and has become a big country in the production and use of pesticides. "Monitoring and Analysis of China's Pesticide Market Output in 2012" shows that my country produced 3.549 million tons of pesticides in 2012, ranking first in the ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 史薇邓东阳于红霞王玉婷
Owner NANJING UNIV
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