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Test strip for screening specific fungi allergen IgE and preparation method thereof

A technology for fungi and test strips, which is applied in the field of test strips for specific fungal allergen IgE screening and their preparation, can solve the problems of cumbersome operation, poor sensitivity and precision, long test time, etc. Simple, accurate, and low-cost results

Active Publication Date: 2014-08-20
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] Example The test strip used for IgE screening of specific fungal allergens and its preparation method

[0028] The preparation of test strips for IgE screening of specific fungal allergens includes: preparation of antigen solution to be labeled, preparation of colloidal gold solution to be labeled, colloidal gold labeled cat hair antigen, purification of colloidal gold cat hair antigen, colloidal gold cat hair antigen Size identification of gold protein particles and coating of test strips. Specific steps are as follows:

[0029] 1. Preparation of antigen solution to be labeled

[0030] The fungal antigen is an Alternaria antigen; the preparation method of the Alternaria antigen is as follows: take 50 g of Alternaria mycelium, degrease with 200 mL of absolute ethanol, repeat the degreasing 5-6 times, and after the degreasing ends, After centrifugation, the centrifuged material is placed in a fume hood, and the residual ethanol is completely evaporated to obtain the Al...

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Abstract

The invention provides a test strip for screening specific fungi allergen IgE (Immunoglobulin E). The test strip is coated with a fungi antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, colloidal gold is used for marking specific fungi allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare the test strip product for allergen detection. The test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

Description

technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for IgE screening of specific fungal allergens and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country and 5-25% of the population in the United States and Europe have suffered from allergic infestation. Among them, young children And teenagers are particularly obvious, and harm is more serious. Allergic reaction (allergy) is a pathological immune response that causes physiological dysfunction and tissue damage when the immune body is exposed to the same allergen again. Involves various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including transplant rejectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/564G01N33/544
CPCG01N33/531G01N33/544G01N33/558G01N33/564G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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