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A method for simultaneously obtaining selenium-enriched solid fermentation mycelia and selenium-enriched Wulingshen fruiting bodies

A technology of solid fermentation and mycelium, applied in the direction of fungi, etc., to achieve the effects of rapid growth, improved use efficiency, and shortened production cycle

Active Publication Date: 2016-03-30
杭州德润全健康产业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fruiting body of selenium-enriched Wuling ginseng and the powder of solid-state fermentation of selenium-enriched wuling ginseng have not been reported.

Method used

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  • A method for simultaneously obtaining selenium-enriched solid fermentation mycelia and selenium-enriched Wulingshen fruiting bodies
  • A method for simultaneously obtaining selenium-enriched solid fermentation mycelia and selenium-enriched Wulingshen fruiting bodies
  • A method for simultaneously obtaining selenium-enriched solid fermentation mycelia and selenium-enriched Wulingshen fruiting bodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Prepare Aspergillus niger liquid medium: glucose 4%, peptone 2%, sodium selenite 1g / L, the rest is water, and the sterilization condition is 121°C for 25min. After cooling to room temperature, inoculate with Aspergillus niger spores. Cultivate at 180 rpm and 35° C. for 72 hours to obtain liquid strains of Aspergillus niger.

[0039] Solid medium configuration: Add 35% water to the broken rice, steam it thoroughly, pour it out and knead it loose, then put it into a fermentation bottle, sterilization conditions: 121°C, sterilize for 45 minutes, then heat and shake to disperse. After cooling to room temperature, the liquid seed of Aspergillus niger was inoculated, and the inoculation amount was 10% (V / W).

[0040] Fermentation culture of Aspergillus niger: inoculate the solid culture of Aspergillus niger at 32°C for 72 hours. After the incubation, sterilize at 121°C for 10 minutes. Shake it off from the heat, and then insert the black stalk bacteria. The inoculum size ...

Embodiment 2

[0046] Prepare Aspergillus niger liquid medium: glucose 4%, peptone 2%, sodium selenite 2g / L, sterilization conditions: 121°C, 25min. After cooling to room temperature, inoculate with Aspergillus niger spores. Incubate at 180 rpm for 72 hours at 35°C. That is, the liquid strain of Aspergillus niger was obtained.

[0047] Solid medium configuration: Add 35% water to the broken rice, steam it thoroughly, pour it out and knead it, add 10% of the broken rice amount of soybean powder, and then put it into a fermentation bottle. Sterilization conditions: 121°C, 45min , just shake off the heat. After cooling to room temperature, the liquid seed of Aspergillus niger was inoculated. The inoculum size was 10% (V / W).

[0048] Fermentation culture of Aspergillus niger: inoculate the solid culture of Aspergillus niger at 32°C for 72 hours. After the incubation, sterilize at 121°C for 10 minutes. Shake it off from the heat, and then insert the black stalk bacteria. The inoculum size ...

Embodiment 3

[0054] Prepare Aspergillus niger liquid medium: glucose 4%, peptone 2%, sodium selenite 4g / L, sterilization conditions: 121°C, 25min. After cooling to room temperature, inoculate Aspergillus niger spores. Incubate at 180 rpm for 72 hours at 35°C. That is, the liquid strain of Aspergillus niger was obtained.

[0055] Solid medium configuration: rice flour: edible bran = 5:1, water content 50%, then put into a fermentation bottle, sterilization conditions: 121°C, sterilize for 45 minutes, then heat and shake to disperse. After cooling to room temperature, the liquid seed of Aspergillus niger was inoculated. The inoculum size was 10% (V / W).

[0056] Fermentation culture of Aspergillus niger: inoculate the solid culture of Aspergillus niger at 32°C for 72 hours. After the incubation, sterilize at 121°C for 10 minutes. Shake it off from the heat, and then insert the black stalk bacteria. The inoculum size was 10% (V / W).

[0057] Cultivation of C. nigriscens: Inoculate the sol...

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Abstract

The invention discloses a method for simultaneously obtaining selenium-enriched solid fermentation mycelia and high-yield selenium-enriched xylariasp sporocarp. The method comprises the steps of: S1, preparing a liquid culture medium rich in inorganic selenium, sterilizing and then inoculating aspergillus niger spores to be subjected to liquid culture, and thus obtaining liquid strains of aspergillus niger; S2, preparing a solid culture medium, inoculating liquid culture medium of aspergillus niger obtained in the step S1 after sterilizing, and performing solid-state fermentation to the aspergillus niger; S3, preparing a liquid culture medium, inoculating xylaria nigripes seeds, performing liquid culture, and thus obtaining liquid strains of xylaria nigripes; S4, after sterilizing the solid yeast of aspergillus niger obtained in the step S2, inoculating the liquid strains of xylaria nigripes obtained in the step S3 and then culturing; S5, harvesting xylariasp sporocarp grown in the step S4, and thus obtaining the xylariasp sporocarp rich in inorganic selenium; S6, washing residual solid culture medium after harvest, drying after removing the unconverted inorganic selenium, and thus obtaining selenium-enriched xylariasp which has been subjected to solid-state fermentation. According to the method, while the high-yield selenium-enriched sporocarp is obtained, the selenium-enriched solid fermentation mycelia are also obtained.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for simultaneously obtaining selenium-enriched solid fermentation mycelia and selenium-enriched Wulingshen fruiting bodies. Background technique [0002] Wulingshen, also known as Leizhenzi, Diao Jinzhong, is a precious Chinese herbal medicine, which has the functions of dehumidification, calming and tranquilizing. Hematopoiesis and improving the immune function of the body have obvious effects on the treatment of spleen deficiency, lack of food, postpartum and postoperative blood loss, postpartum milk loss, gastroptosis, sickness, palpitations, insomnia, convulsions in children, and traumatic injuries. Studies have shown that Wuling ginseng is the sclerotia formed by Xylarianigripes (KI.) Sacc in abandoned termite nests, and is also called chicken fir eggs among the people. [0003] In the wild, the mycelium of C. nigritiformis is usually densely distributed on t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05D9/02
Inventor 陈小林赵敏邵丽芳王瑞
Owner 杭州德润全健康产业发展有限公司
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