A kind of anticoagulant pharmaceutical composition and its application
A composition and anti-coagulation technology, applied in the field of medicine, can solve the problems of anti-coagulation or anti-thrombosis without rafenolactone, and achieve remarkable effects and prevent stroke or other thrombotic diseases
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Embodiment 1
[0013] Example 1: Effect test of rafenolactone on mouse bleeding and coagulation time
[0014] 36 SPF level healthy Kunming mice, half male and half male, body weight (20 2) g. 36 mice were randomly divided into three groups: normal saline group (10ml / kg), high-dose rafenolactone group (15mg / kg), low-dose group (5mg / kg), 12 in each group. 40 minutes after the intraperitoneal injection of the mouse, use scissors to cut off the tail tip of the mouse at 0.5 cm, and use a stopwatch to time when the bleeding begins, and stick the bleeding at the broken end with filter paper every 30 seconds until the red blood is completely gone on the filter paper. is the bleeding time of the mouse. The longest bleeding time was observed for 60 minutes, and if the blood was still bleeding after 60 minutes, it was calculated as 60 minutes.
[0015] One hour after the administration, the mouse's eyeball was picked to collect blood, and the coagulation time was measured by the glass slide method;...
Embodiment 2
[0020] Example 2: Effect test of rafenolactone on thrombus formation in rat common arteries
[0021] 30 SD rats, half male and half male, body weight (300 20) g. SD rats were randomly divided into the following three groups: normal saline group (5ml / kg), high-dose rafenolactone group (10mg / kg), low-dose group (3.3mg / kg), 10 rats in each group. After anesthetized by intraperitoneal injection of 10% chloral hydrate solution 4.5ml / kg, the rats were fixed on a small animal dissecting board, the left external jugular vein and the right common carotid artery were bluntly separated, and corresponding doses of the recipients were injected from the left external jugular vein. After 5 minutes of drug testing, a YLS-14B thrombogenesis instrument was used to induce thrombus formation in the right common carotid artery of rats by electrical stimulation (current 1mA, stimulation time 5min), observe the thrombus obstruction of the right common carotid artery, and print out relevant data. ...
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