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A method for extracting peony flower total flavonoids from peony flower

A technology of peony flowers and total flavonoids is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., which can solve the problems of less research and achieve the effects of simple method, clear flavonoid composition, and improved product purity.

Active Publication Date: 2016-05-04
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still relatively few studies on the preparation and isolation of flavonoids from peony flower, the confirmation of material basis and the research on activity.

Method used

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  • A method for extracting peony flower total flavonoids from peony flower
  • A method for extracting peony flower total flavonoids from peony flower
  • A method for extracting peony flower total flavonoids from peony flower

Examples

Experimental program
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Effect test

Embodiment 1

[0070] A method for extracting total flavonoids of peony from peony, comprising the following steps:

[0071] 1) Extraction with ethanol solution

[0072] Pick the peony flower raw material, remove the calyx and receptacle to obtain the peony petals, dry the peony petals at room temperature and then pulverize, weigh 100g of the pulverized peony petals, and then add it to a 90% ethanol solution with a mass concentration 20 times its weight Soak in medium for 6 hours, perform ultrasonic extraction for 40 minutes after soaking, filter after extraction, collect the filtrate, and add 15 times its weight to the obtained filter residue with an ethanol solution with a mass concentration of 70%, repeat the ultrasonic extraction and filtration operation 2 times , sonicate for 40 minutes each time, combine all the filtrates, concentrate under reduced pressure until there is no solvent, and extract the extract for subsequent use;

[0073] 2) Resin enrichment of crude flavonoids from peon...

Embodiment 2

[0081] A method for extracting total flavonoids of peony from peony, comprising the following steps:

[0082] 1) Extraction with ethanol solution

[0083] Pick the peony flower raw material, remove the calyx and receptacle to obtain the peony petals, dry the peony petals at room temperature and then pulverize, weigh 200g of the pulverized peony petals, and then add 10 times its weight to a 70% ethanol solution Soak in medium for 48 hours, perform ultrasonic extraction for 20 minutes after soaking, filter after extraction, collect the filtrate, and add 90% ethanol solution with a mass concentration of 10-20 times the weight of the filter residue to the obtained filter residue, repeat the ultrasonic extraction and filtration operations 2 times, ultrasonication for 20min each time, all the filtrates were combined, concentrated under reduced pressure to no solvent, and the extracted extract was obtained, a total of 32.6g, and set aside;

[0084] 2) Resin enrichment of crude flavo...

Embodiment 3

[0092] A method for extracting total flavonoids of peony from peony, comprising the following steps:

[0093] 1) Extraction with ethanol solution

[0094] Pick the peony flower raw material, remove the calyx and receptacle to obtain the peony petals, dry the peony petals at room temperature and then pulverize, weigh 1000g of the pulverized peony petals, and then add it to an 80% ethanol solution with a mass concentration 15 times its weight Soak in medium for 24 hours, perform ultrasonic extraction for 30 minutes after soaking, filter after extraction, collect the filtrate, and add an ethanol solution with a mass concentration of 15 times the weight of the filter residue to the obtained filter residue, and repeat the ultrasonic extraction and filtration operation twice , sonicate for 30 minutes each time, combine all filtrates, concentrate under reduced pressure until there is no solvent, and obtain 170 g of extract extract, which is set aside;

[0095] 2) Resin enrichment of c...

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Abstract

The invention mainly discloses a method for extracting total flavonoids from peony flowers, which includes extraction with ethanol solution, resin enrichment of crude total flavonoids from peony flowers, precipitation enrichment from refined products of total flavonoids from peony flowers, and further purification of total flavonoids from peony flowers. In a big step, the present invention effectively improves the product purity by first adding lead acetate to precipitate and then H2S to remove lead, reaching more than 96%, and the yield reaches more than 1.0%. The obtained flavonoids have a clear composition and are suitable for industrial production. , the activity test of flavonoids obtained shows a better effect of promoting blood circulation and removing blood stasis. The method can be used for large-scale extraction of total flavonoids from peony flowers, which lays a foundation for the future research and development of peony flowers and the flavonoids contained therein.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective components in natural products, and in particular relates to a method for extracting total flavonoids of peony flowers from peony flowers. Background technique [0002] Peony is native to the Qinling Mountains and Daba Mountains in western my country. It is a small perennial deciduous shrub. The whole body of peony is a treasure, and its roots, stems, leaves, flowers, seeds, etc. are all of great value. At present, a lot of work has been done on peony cultivation and research methods. In terms of peony research, Wu Longqi et al. 2005,36(10):77-80] research has determined that peony anthocyanins are anthocyanin pigments, and the suitable conditions for extracting peony anthocyanins are: the leaching agent is acidic absolute alcohol with a volume fraction of hydrochloric acid of 1%. The temperature is 50°C, and the leaching time is 3h. [Fan Jinling, Zhu Wenxue, Ma H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61P7/02A61K133/00
Inventor 刘普邓瑞雪易军鹏李小方高嘉屿尹卫平柴元武时清亮
Owner HENAN UNIV OF SCI & TECH
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