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Escherichia coli and preparation method thereof

A technology of Escherichia coli and Escherichia coli, applied in the field of microbial strains and their preparation, can solve the problems of epidemic disease, loss of immunogenicity, toxic strains, etc., and achieve the effects of good immune effect and broad industrial development prospects.

Inactive Publication Date: 2014-08-27
HEBEI KEXING PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But such vaccines are not ideal, because the weakened strains may undergo back mutations and become toxic, or lose their immunogenicity, and inactivating inappropriate vaccines can also cause epidemics

Method used

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  • Escherichia coli and preparation method thereof
  • Escherichia coli and preparation method thereof
  • Escherichia coli and preparation method thereof

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Embodiment Construction

[0065] The accompanying drawings show the embodiments of the present invention. The embodiments of the present invention will be further described below in conjunction with the accompanying drawings, but they are not intended to limit the present invention. The scope of protection of the present invention is based on the contents of the claims. The replacement of the equivalent technical means mentioned above does not depart from the protection scope of the present invention.

[0066] An Escherichia coli, the preservation number of which is CGMCC No.8574.

[0067] For the Escherichia coli (Escherichia coli) involved in this example, the applicant has submitted to the General Microorganism Center of the Microbiological Culture Preservation Management Committee on December 13, 2013 for preservation. The address of the preservation unit is located in Beichen West, Chaoyang District, Beijing. In the Institute of Microbiology, Chinese Academy of Sciences, No. 1, Yard 3, the deposit...

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Abstract

The invention belongs to microbial strains and preparation methods of the microbial strains, and particularly relates to Escherichia coli and a preparation method of the Escherichia coli. A cloned iss gene segment in outer membrane protein of Escherichia coli pathogenic bacteria of a coded bird is regrouped in an expression plasmid pEGX-6p-1 after BamHI and SalI double enzyme digestion, and a pronucleus expression plasmid pEGX-6p-1 / iss is built; the pronucleus expression plasmid pEGX-6p-1 / iss is transformed into Escherichia coli BL21-DE3, and regrouped Escherichia coli BL21-DE3 / pEGX-6p-1 / iss is built. The technical problem that the vaccine broad-spectrum performance is poor in the prior art is solved. The Escherichia coli has the advantages of being good in broad spectrum performance, good in immune effect, wide in industrialized development prospect and the like.

Description

technical field [0001] The invention belongs to microbial strains and preparation thereof, in particular to Escherichia coli and a preparation method thereof. Background technique [0002] Colibacillosis is a general term for diseases caused by certain pathogenic serotype strains of Escherichia coli. Avian colibacillosis refers to the local or systemic infection caused partially or entirely by avian pathogenic Escherichia coli (APEC), including E. coli sepsis, E. coli granuloma (Hjarre disease), air bag disease (chronic respiratory disease (CRD), E. coli cellulitis (ie, inflammatory process), puffy head syndrome, E. coli peritonitis, E. coli salpingitis, E. coli osteomyelitis / synovitis (ie, turkey osteomyelitis syndrome), E. coli panphthalmitis, and E. coli omphalitis / yolk sac infection. Escherichia coli mainly causes intestinal diseases in mammals, and when poultry are infected with highly pathogenic Escherichia coli and their defense ability is insufficient to resist or ...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/31C12N15/70C12R1/19
Inventor 杨广平陈淑芳马兴树张鑫李忠鹏王洋武路刚
Owner HEBEI KEXING PHARMA
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