Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Screening kit and method for early drought stress induced festuca arundinacea DREB2 (dehydration responsive element binding protein 2) gene

A technology for tall fescue and drought stress, which can be applied in the fields of biochemical equipment and methods, microbial determination/inspection, etc., and can solve the problems of lack of information related to the molecular mechanism of drought resistance.

Inactive Publication Date: 2015-02-25
HUNAN AGRICULTURAL UNIV +1
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims to provide a screening kit and method for tall fescue DREB2 gene under early drought stress in view of the current lack of relevant information on the molecular mechanism of drought resistance of tall fescue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Screening kit and method for early drought stress induced festuca arundinacea DREB2 (dehydration responsive element binding protein 2) gene
  • Screening kit and method for early drought stress induced festuca arundinacea DREB2 (dehydration responsive element binding protein 2) gene
  • Screening kit and method for early drought stress induced festuca arundinacea DREB2 (dehydration responsive element binding protein 2) gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1. Treatment of tall fescue drought stress samples

[0028] According to the previous experimental results of the chlorophyll fluorescence kinetic parameters and relative electrical conductivity of seedlings under PEG stress, it was determined that the leaves of tall fescue were stressed with 20% PEG6000 for 0h, 6h, 12h, 18h and 24h respectively. Transcriptome sequencing RNA sources.

[0029] 2. High-throughput transcriptome sequencing analysis

[0030] According to the conventional Trizol method, the RNA of the tall fescue leaves after the drought stress treatment was extracted, and the mixed RNA of each treatment and the control group was used as the sample source for total transcriptome sequencing, using the conventional high-throughput sequencing technology platform IlluminahiSeq TM In 2000, deep sequencing of the transcriptome was carried out, and Trinity software was used for de novo splicing, and the short reads (reads) obtained by sequencing were spliced ​​into...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a screening kit and a method for an early drought stress induced festuca arundinacea DREB2 (dehydration responsive element binding protein 2) gene. According to the method, PEG (polyethylene glycol) 6000 drought stress treated (0h, 6h, 12h, 18h and 24h) festuca arundinacea is used as an experimental material, mixed transcriptome sequencing analysis is performed by utilizing an RNA-seq (ribonucleic acid-sequencing) technology of an Illumina / Solexa sequencing platform, and a registered DREB2 gene sequence is used as a probe to perform Local Blast, so that the early drought stress induced festuca arundinacea DREB2 gene is screened. The method can be used for quickly and comprehensively screening the drought stress induced festuca arundinacea DREB2 candidate gene, and has an important guiding magnificence to drought-resistance mechanism researches and plant resistant breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a screening kit and method for inducing tall fescue DREB2 candidate genes by early drought stress. Background technique [0002] DREB2s (Dehydration Responsive Element Binding Protein2) is a kind of plant-specific transcription factor, which belongs to the AP2 / EREBP transcription factor family and plays an important role in the plant stress signal transduction pathway. Liu et al. and Kasuga et al. first cloned the DREB2A transcription factor from Arabidopsis thaliana (L.) Heynh.], and found that it is related to the drought response element CRT / DRE (C-Repeat / Dehydration-Responsive Element, core sequence: A / GCCGAC) or GCC-BOX (core sequence: TAGCCGCCA) specifically binds, participates in regulating the expression of tolerance-related genes such as drought, high-salt and low temperature, and plays an important role in the process of plant abiotic stress response. The seque...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2535/122C12Q2537/165
Inventor 张志飞张瑜王增裕陈桂华赵志丽
Owner HUNAN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com