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About natural killer t cell activator polypeptide and its application

An acute lymphocyte, sequence technology, applied in the direction of peptides, peptide/protein components, medical preparations containing active ingredients, etc., can solve the problems that have not yet been developed, and achieve the effect of increasing the survival rate

Inactive Publication Date: 2016-06-01
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no developed natural killer T cell activator polypeptide drug for the treatment of acute lymphoblastic leukemia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Chemical Synthesis of Peptides

[0015] The peptides were synthesized using Fmoc chemistry. The synthesis reaction proceeds from the C-terminus to the N-terminus. There are free amino groups on the Rink medium (available from AdvancedChemTech), and the amino acids are connected sequentially. During each ligation step, the amino acid residues are activated, and the activation mixture contains 4 times as many HBTU, HOBt, DIEA and Fmoc-amino acids as there are free amino groups on the medium. After each amino acid connection reaction, a mixture of pyridine / acetic acid / N-methylimidazole (4:1:0.5) was used to block the unconnected free amino group for 10 minutes. After each amino acid ligation reaction and before the next amino acid is ligated, the Fmoc-group on the medium must be removed, and the Fmoc-group is removed using dimethylformamide containing 20% ​​piperidine, which takes 15 minutes. Finally, after all amino acid residues are linked sequentially, the peptide is ...

Embodiment 2

[0018] In Vivo Viability of Natural Killer T Cell Activator Polypeptide 3 Using Endotoxic Shock Model

[0019] Before establishing the endotoxin shock model, we first determined the LD50 of LPS (E.coli0111: B4, purchased from sigma company) mice as 50 μg per mouse, and we used 100 μg per mouse in the experiment, so that the control All mice in the group died. In our previous scientific research work, we found that Regasepin2 (another peptide inhibitor, published) can improve the survival rate of C57BL / 6 mice during endotoxin shock. In order to establish endotoxin shock model in our mice, we did a positive control experiment with Regasepin2. The mice in the control group were injected with 100 μg LPS, while the mice in the Regasepin2 experimental group were injected with 0.7 mg of polypeptide 5 minutes after LPS injection. By making a Kaplan-Meier survival curve, it was found that Regasepin2 can effectively protect mice injected with 100 μg and improve the survival rate. Aft...

Embodiment 3

[0021] IC50 value of natural killer T cell activator polypeptide 3 matrix metalloproteinase-9 in vitro.

[0022] Recombinant human matrix metalloproteinase-9 is expressed by Sf9 insect cells. The enzyme is activated with 0.01 μM matrix metalloproteinase-3 active site, and the buffer used is 100mMTris / HCl, pH7.4, 100mMNaCl, 10mMCaCl 2 and 0.01% Tween-20. The concentration of matrix metalloproteinase-9 during activation was 92ng / μl (1μM). Enzyme activity is detected by cleaving the fluorescently generated peptide substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH 2 And detect the generated fluorescence value (excitation wavelength = 328nm, detection wavelength = 392nm). All detections were carried out in 100μl reaction system at 37°C. The calculated IC50 value was 1.87 μM.

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PUM

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Abstract

The invention relates to the field of drugs and particularly relates to a polypeptide capable of activating natural killer T cells, inhibiting matrix metalloproteinases-9 and relieving destruction of acute inflammatory reaction to an organism. The polypeptide has the sequence as follows: VVHPLSSARWGGRR which is a brand new sequence. The polypeptide can be used for inhibiting the activity of matrix metalloproteinases-2 in vitro on the level of 1 micromole and increasing the survival rate of endotoxin shock rats in an in-vivo test so as to have a potential novel drug development value.

Description

technical field [0001] The invention relates to natural killer T cell activator polypeptide 3 and its application, in particular to a polypeptide capable of activating natural killer T cells and treating acute lymphocytic leukemia. Background technique [0002] Acute lymphoblastic leukemia (ALL) is a progressive malignant disease characterized by large numbers of immature white blood cells that resemble lymphoblasts. These cells can be found in the blood, bone marrow, lymph nodes, spleen and other organs. Acute lymphoblastic leukemia accounts for 80% of acute leukemia in children, and the peak incidence is between 3 and 7 years old. ALL can also occur in adults and accounts for 20% of all adult leukemias. In recent years, with the deepening of medical research, great progress has been made in the understanding and treatment of acute lymphoblastic leukemia. Biological targeted therapy for tumors is very promising. Targeted therapy is to block a certain process of tumorigen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P35/02
Inventor 刘明军滕金龙
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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