Method for determining fatty-acid components of tissues of fish body

Abstract

The invention discloses a method for determining the fatty-acid components of tissues of a fish body. The method comprises the following steps: taking the following tissues of the fish body in percentage by weight: 0.2-0.5% of abdominal fat, 0.8-1.0% of liver, 1-2% of muscle and 1.5-1.8% of gill, and then adding chloroform methanol mixing liquid for grinding; then extracting fat solution by returning and filtering; then blow-drying the solution by utilizing nitrogen and extracting crystallized fat; then dissolving the crystallized fat in potassium hydroxide methanol solution, introducing nitrogen for resisting oxidation and cooling the wall of a container for reducing the volatilization; then esterifying the fat into the fatty acid, then dissolving the fatty acid by using n-heptane; and then taking supernate for centrifugal treatment, then taking and conveying supernate into a gas chromatograph to obtain a chromatogram, and calculating the relative contents of the fatty acid components by the chromatogram through an area normalization method. Therefore, the method disclosed by the invention has the advantages that the recognition for the fat structure of the fish can be realized, so that the fish can be further protected and cultured.

Description

technical field [0001] The invention relates to the field of fish tissue fatty acids, in particular to a method for determining fatty acid components of fish tissue. Background technique [0002] At present, wild fish are facing more and more threats, many of which are facing extinction, or the farming of other common fish encounters difficulties in feeding and matching nutrients. Although the fish body is rich in fatty acids, some fatty acids can be biosynthesized by the fish itself, but some cannot be synthesized or the amount of synthesis is very small, far from meeting the needs of fish growth and development stages, and must be supplemented by external sources. Therefore, it is of great significance to study and measure the fatty acid composition, physiological function and requirement of fish to improve the yield and quality of farmed fish. [0003] In order to protect the race and long-term survival of wild fish and fish farming, it is necessary not only to constantl...

AI Technical Summary

Problems solved by technology

But the current method for extracting fish tissue fat adopts the method of nitrogen blowing to dry the chloroform-methanol mixed solution, and does not do other treatments to the chloroform-methanol mixed solution, which causes the nitrogen blowing time to be too long, and the chloroform-methanol mixed solution is difficult to remove due to the refrigeration effect of nitrogen. Volatility affects the results of fatty acid determination, and when extracting fish tissue fatty acids, there is no uniform standard for tissue sampling volume, which affects the determination of fatty acid components in fish tissues, wastes resources, and has low accuracy