Functions and use of zinc finger protein A20 in treating fatty liver and type 2 diabetes mellitus

A zinc finger protein and fatty liver technology, which is applied in the field of preparation of drugs for the prevention, alleviation and/or treatment of fatty liver and type 2 diabetes, and can solve problems such as etiology and pathogenesis that have not been fully elucidated

Inactive Publication Date: 2014-09-17
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole society has invested huge manpower and financial resources in the research of diabetes. The etiology and pathogenesis of diabetes and its complications have not been fully elucidated, and the prevention and treatment have yet to be perfected.

Method used

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  • Functions and use of zinc finger protein A20 in treating fatty liver and type 2 diabetes mellitus
  • Functions and use of zinc finger protein A20 in treating fatty liver and type 2 diabetes mellitus
  • Functions and use of zinc finger protein A20 in treating fatty liver and type 2 diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Fatty liver and type 2 diabetes model (diet induced obesity, DIO) obtained in mice

[0033] (1) Grouping of experimental animals: 8-week-old, male, WT mice and A20-KO mice were selected and given two special feeds, D12942 high-fat diet (Highfat diet, HFD) and D12450B low-fat diet (Normal chow, NC) feeding, that is, WT NC group, KO NC group, WT HFD group, KO HFD group, a total of 4 groups.

[0034] (2) The operation process of the model induced by high-fat feed:

[0035] WT and KO mice were used to establish DIO models, and phenotype correlation analysis was performed to clarify the role of A20 gene on fatty liver and type Ⅱ diabetes. 8-week-old, male, WT mice and A20-KO mice were selected and fed with two special feeds, D12942 high-fat diet (Highfat diet, HFD) and D12450B low-fat diet (Normal chow, NC), respectively, WT NC group, KO NC group, WT HFD group, KO HFD group, a total of 4 groups. The food intake of the mice was recorded in detail every week, and ...

Embodiment 2

[0036] Example 2 Determination of mouse body weight and blood glucose level

[0037] (1) Fasting body weight and food intake detection of mice

[0038] 1) Weight detection

[0039] ①Fasting: Fast the mice to be tested at 8:00 am (without water), and start the experimental operation at 2:00 pm.

[0040] ② Weighing: Weigh at the 0th week, 4th week, 8th week, 12th week, and 16th week, put a small plastic bucket on the dynamic electronic balance, pick up the mouse, put it into the weighing bucket, and measure Weight record data. Feed amount detection: After the weighing operation is completed, add feed to the mice, and record the amount of feed for the mice on the dynamic electronic balance.

[0041] (2) Fasting blood glucose level detection experiment

[0042] All the mice to be tested were fasted from 8:00 am to 2:00 pm (without water), that is, the experimental operation was started after 6 hours of fasting.

[0043] ① Blood glucose meter preparation: Check the battery of ...

Embodiment 3

[0048] Example 3 Intraperitoneal glucose tolerance test (IPGTT)

[0049] At the 14th week of the experiment, the intraperitoneal glucose injection test (IPGTT) was performed to evaluate the glucose tolerance of the mice.

[0050] (1) Before measuring blood glucose, measure the fasting body weight of the mice, and calculate the injection volume of glucose based on 10 μL / g.

[0051] (2) First test the fasting blood glucose at 0 minutes before the glucose injection, and inject the glucose solution intraperitoneally quickly after the test is completed.

[0052] (3) Operation method of intraperitoneal injection: ①Fix the mouse; grab the mouse, grasp the tail of the mouse with the little finger and ring finger of the left hand, and grasp the neck of the mouse with the other three fingers, so that the mouse's head is down, and the The abdomen of the mouse is fully exposed. ②Needle positioning and injection: insert the needle from the side of the abdomen, hold the syringe with the r...

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Abstract

The invention discloses functions and use of zinc finger protein A20 in treating fatty liver and type 2 diabetes mellitus, and belongs to the fields of the functions and the uses of genes. The functions of the A20 are studied by use of high fat diet-induced models; results indicate that the body weight and the fasting blood glucose level of A20 knockout mice in an HFD (High Fat Diet) group both are higher than those of WT mice in a control group; intraperitoneal injection glucose tolerance experiments show that the tolerance ability of the A20 knockout mice to the glucose is obviously weakened; the general liver appearance, the liver weight, the liver / weight ratio, the lipid component pathological staining result and the like of the mice all indicate that the fatty liver lesion of the A20-KO mice in the HFD group is obviously deteriorated and the lipid accumulation is remarkably increased, and the results indicate that the fatty liver and the type 2 diabetes mellitus can be remarkably worsened due to A20 knockout. For the effects of the A20, the A20 can be applied to preparing medicines for preventing, relieving and / or treating the fatty liver and / or the type 2 diabetes mellitus.

Description

technical field [0001] The present invention belongs to the field of gene function and application, and in particular relates to the function and application of a zinc finger protein A20 in the treatment of fatty liver and type II diabetes, and the use of A20 as a target gene in the preparation of prevention, alleviation and / or treatment of fatty liver and type II diabetes. Drug application in type 2 diabetes mellitus. Background technique [0002] Fatty liver and diabetes are a chronic metabolic disease that seriously endangers human health. It is a metabolic disease and a chronic, low-grade inflammatory disease. The number of its patients is increasing rapidly with the improvement of people's living standards, population aging, changes in lifestyle, and advances in diagnostic techniques. In 2002, it was found in the national nutrition survey that the prevalence of diabetes among residents aged 18 and over in my country was 2.6%, while in the sample survey of diabetes in 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P1/16A61P3/10
Inventor 李红良张晓东汪涛杜成
Owner WUHAN UNIV
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