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A kit for detecting alanyl aminopeptidase

A kind of technology of alanyl amino group and reagent kit, which is applied in the field of medical inspection and measurement, can solve the problems of poor accuracy and achieve the effects of high accuracy, wide application range and good precision

Active Publication Date: 2015-12-02
CHANGCHUN HUILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the object of the present invention is to provide a method and a kit for detecting alanyl aminopeptidase with high accuracy for the problems such as poor accuracy of existing methods for detecting alanyl aminopeptidase

Method used

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  • A kit for detecting alanyl aminopeptidase
  • A kit for detecting alanyl aminopeptidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: A kit for detecting alanyl aminopeptidase according to the present invention.

[0042] The test kit for detecting alanyl aminopeptidase of the present invention is a liquid single reagent, comprising: 100mmol / L pH7.5~8.0 Tris-HCL buffer solution, 10mmol / LL-alanyl-p-nitroaniline, 0.2mmol / L Sodium azide, 0.65mmol / LMgCl 2 And 0.5mmol / L zinc sulfate.

Embodiment 2

[0043] Embodiment 2: A kit for detecting alanyl aminopeptidase according to the present invention.

[0044] The test kit for detecting alanyl aminopeptidase of the present invention is double reagent, the first reagent comprises 100mmol / LPH7.5~8.0Tris-HCL buffer solution, 0.65mmol / LMgCl 2 and 0.5mmol / L zinc sulfate, the second reagent includes 10mmol / LL-alanyl-p-nitroaniline, 0.2mmol / L sodium azide.

Embodiment 3

[0045] Example 3: A method for detecting alanyl aminopeptidase.

[0046] Set the reaction temperature of the automatic biochemical analyzer to 37°C, the reaction method is the rate method, the main wavelength of the measurement is 405nm, the reaction direction is positive reaction, the delay time is 90s, the measurement time is 120s, and the theoretical factor is 2525. Then take the sample to be tested and the reagents in the kit and place it in a fully automatic biochemical analyzer, the instrument automatically adds samples and mixes, detects and records the absorbance change value at a wavelength of 405nm. According to the calculation formula C 样 =△A 样 / min×K, calculate the concentration of alanyl aminopeptidase in the sample to be tested, wherein, C 样 is the concentration of the sample to be tested; △A 样 / min is the absorbance change rate of the sample per minute; K is a theoretical factor, which has been set to 2525.

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Abstract

The invention relates to the technical field of medical detection and discloses a method and a kit for detecting alanylaminopeptidase. The method for detecting the alanylaminopeptidase is simple to operate and does not need to dilute a reagent and a sample in advance; a working reagent does not need to be prepared and can be directly used on various automatic biochemical analyzers to quantitatively determine the activity of the alanylaminopeptidase in human blood serum or urine; the determination of the urine is basically not influenced by urine amino acid and ammonia. An experiment shows that the method for detecting the alanylaminopeptidase is high in accuracy of a detection result, good in sensitivity, good in precision and wide in linear range. The kit for detecting the alanylaminopeptidase provided by the invention is good in stability and long in storage life; the kit can be conveniently used for detecting on the full-automatic biochemical analyzer, has a wide applicable range, is convenient to popularize and use and can be widely used for determining the content of the alanylaminopeptidase in the human blood serum or the urine by various hospitals, sanitary prevention departments and medical biological research and development institutions.

Description

technical field [0001] The invention relates to the technical field of medical testing and determination, in particular to a method and a kit for detecting alanyl aminopeptidase. Background technique [0002] Alanyl aminopeptidase, APP for short, is an isozyme of leucine aminopeptidase (LAP). The changes of alanyl aminopeptidase activity in serum and urine are closely related to certain pathological states of the body. Some studies have shown that serum AAP determination can be used for the diagnosis of hepatobiliary diseases, liver tumors and fatty liver. Intrahepatic cholestasis, severe hepatitis, obstructive jaundice, primary and secondary liver cancer can all cause a significant increase in serum AAP, which is more sensitive than other indicators. Other studies have shown that the determination of urinary AAP can be applied to the diagnosis of glomerulonephritis and early renal tubular injury. In renal transplantation rejection, long-term use of nephrotoxic drugs to t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/52
CPCG01N21/31G01N33/573
Inventor 董理
Owner CHANGCHUN HUILI BIOTECH CO LTD