Cellulase-catalyzed method for extracting spreading hedyotis herb polysaccharides

A technology for extracting Hedyotis diffusa and enzymatic method is applied in the field of extraction and production technology of active components of traditional Chinese medicine, and can solve the problems of low accuracy, inability to investigate the interaction of factors, and damage to the activity of polysaccharides by extraction temperature, so as to improve the yield and extract Accurate and reliable effect of condition parameters

Active Publication Date: 2014-09-24
HUNAN AGRICULTURAL UNIV
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AI Technical Summary

Problems solved by technology

At present, the main methods of polysaccharide extraction include hot water extraction, acid extraction, alkali extraction, ultrasonic extraction and microwave extraction, etc., these methods have certain shortcomings, such as low extraction rate and low purity. , or the extraction temperature is too high to destroy the activity of polysaccharides, or cause the degradation of polysaccharide molecules, etc.
At present, polysaccharide extraction processes are mostly uniform design and orthogonal design. These designs use a single factor investigation method to determine the process conditions by fixing other factors and changing one factor. The interaction between factors cannot be investigated, and it is also difficult to investigate multiple factors. Interaction relationship between the response and the factor, so the precision is not high

Method used

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  • Cellulase-catalyzed method for extracting spreading hedyotis herb polysaccharides

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Embodiment 1

[0042] Example 1 (1) Extract Hedyotis diffusa by hot water immersion, pulverize, pass through a 40-mesh sieve, weigh Hedyotis diffusa powder, and mix it with water in a ratio of 8 to 12; adjust the pH to about 7 with phosphate buffer , add 1.5-2.5% cellulase to the feed solution, mix well, stir and extract in a water bath at 55°C for 60 minutes;

[0043] (2) Enzyme inactivation: the extracted solution was inactivated at 90° C. for 10 minutes, and the filtrate was obtained by suction filtration;

[0044] (3) Add cellulase hot water to the filter residue for the second leaching: mix the filter residue obtained from the first extraction with water in a ratio of 1:8 to 12 by weight; adjust the pH with phosphate buffer to Around 7, add 1.5-2.5% cellulase, mix well, stir and extract in a water bath at 55°C for 60 minutes;

[0045] (4) Enzyme inactivation: the second filtrate was inactivated at 90° C. for 10 minutes, and the filtrate was obtained by suction filtration; the two filtr...

Embodiment 2

[0058] Example 2 (1) soak Hedyotis diffusa in hot water to extract Hedyotis diffusa and pulverize it, pass through a 40-mesh sieve, weigh 20 grams of Hedyotis diffusa powder, add water to 200 ml, and mix evenly; use phosphate buffer to adjust the pH to about 7, Add 2% cellulase to the feed solution, mix well, stir and extract in a water bath at 55°C for 60 minutes; perform a second extraction on the filter residue, add water to 200 ml, mix well, and adjust the pH to about 7 with phosphate buffer , add 2% cellulase, mix well, stir and extract in a water bath at 55°C for 60 minutes, filter with suction to obtain the filtrate, combine the two filtrates, inactivate the enzyme, and inactivate the enzyme at 90°C for 10 minutes on the filtrate.

[0059] (2) Concentration: Concentrate the filtrate to 30-40 milliliters under vacuum rotation and reduced pressure, and put it to room temperature;

[0060] (3) The first alcohol precipitation: add 95% ethanol to the concentrated solution pr...

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Abstract

The invention belongs to the field of the production process of extracting the active ingredients of Chinese herbs and in particular relates to a response surface analysis-optimized and cellulase-catalyzed method for extracting spreading hedyotis herb polysaccharides and optimum extraction process conditions. The spreading hedyotis herb polysaccharides are extracted by use of the cellulase-catalyzed method which comprises the steps of cellulase-catalyzed hot water extraction, enzyme deactivation, concentration, alcohol precipitation, deproteinization, alcohol precipitation, depigmentation, alcohol precipitation, extraction rate measurement and the like, namely carrying out hot water extraction twice according to the material-to-liquid ratio of 1: (8-12) and by adding about 2% of cellulase, triple precipitation by use of an 80% alcohol, deproteinization twice by use of trichloroacetic acid and twice depigmentation by use of activated carbon adsorption. The following optimum extraction process conditions for extracting the spreading hedyotis herb polysaccharides are obtained by virtue of a response surface three-dimensional diagram: pH of about 7, a temperature at 55 DEG C and the dosage of the cellulase of 2%; the extraction rate of the spreading hedyotis herb polysaccharides under the conditions is 9.83%.

Description

technical field [0001] The invention belongs to the field of extraction and production technology of active ingredients of traditional Chinese medicines, and in particular relates to a method for extracting Hedyotis diffusa polysaccharides by using response surface analysis to optimize cellulosic enzymatic method and optimal extraction process conditions. Background technique [0002] Polysaccharides, also known as polysaccharides, are a type of natural macromolecular compounds that are polymerized by monosaccharides such as aldose or ketose through glycoside chains. Its molecular weight is generally tens of thousands or even millions, and it is widely present in animal cell membranes. In the cell walls of plants and microorganisms, more than 300 polysaccharides have been extracted from natural products so far, among which the water-soluble polysaccharides obtained from Chinese medicinal materials are the most important. Studies have shown that polysaccharides from tradition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 贺建华符晨星瞿俊勇游思湘刘湘新
Owner HUNAN AGRICULTURAL UNIV
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