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A kind of detection method of naphthylamine compound based on chemiluminescence

A technology of chemiluminescence and detection methods, which is applied in the field of analytical chemistry and optical DNA biosensing, can solve the problems of no report on naphthylamine compounds, long electrode preparation cycle time, and large fluctuation of measurement results, reaching the experimental cycle Short, low cost, high reliability effect

Inactive Publication Date: 2018-02-02
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of aromatic amine compounds using DNA as the main body of molecular recognition mostly uses DNA-modified electrodes. Although these DNA biosensors have high sensitivity to aromatic amine pollutants, the disadvantage is that the electrode preparation Long cycle time, relatively high cost, and large fluctuations in measurement results
So far, the use of G-DNA catalytic properties to catalyze the oxidation of luminol by hydrogen peroxide by chemiluminescence and the detection of naphthylamines has not been reported.

Method used

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  • A kind of detection method of naphthylamine compound based on chemiluminescence
  • A kind of detection method of naphthylamine compound based on chemiluminescence
  • A kind of detection method of naphthylamine compound based on chemiluminescence

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Effect test

Embodiment 1

[0026] 1) 20 mM Tris-NaClO at pH=9.0 4 Dissolve the solid G-DNA in the buffer solution, mix it with a vortex shaker, and then add a certain concentration of K to the above system + And hemin solution, put it for 1-2h to form a G-DNA structure with peroxidase-like activity;

Embodiment 2

[0028] Control assay system G-DNA concentration is 100nM, K + Concentration is 10mM, hemin concentration is 2μM, H 2 o 2 The concentration was 5mM, the concentration of luminol was 500μM, and the chemiluminescence intensity of the system with different concentrations of 1-aminonaphthalene (10nmol / L, 30nmol / L, 50nmol / L, 100nmol / L, 200nmol / L and 400nmol / L) was measured. The chemiluminescence intensity is shown in Table 1.

[0029] Table 1 Chemiluminescence intensity after interacting with different concentrations of 1-aminonaphthalene

[0030]

Embodiment 3

[0032] Be that concentration is 100nM G-DNA in the implementation example 2 that concentration is 50nM G-DNA instead, other conditions are with embodiment 2, measure different concentration 1-aminonaphthalene (10nmol / L, 30nmol / L, 50nmol / L, 100nmol / L, 200nmol / L and 400nmol / L) system chemiluminescence intensity. The chemiluminescence intensity is shown in Table 2.

[0033] Table 2 Chemiluminescence intensity after interacting with different concentrations of 1-aminonaphthalene

[0034]

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Abstract

The invention relates to a chemiluminiscence-based detection method of a naphthylamine compound, which belongs to the technical field of analysis chemistry and optical DNA (deoxyribonucleic acid) biological sensing, and relates to a detection method for detecting naphthylamine according to the influence of naphthylamine on a chemiluminiscence signal by adopting peroxidase of G-DNA chain to catalyze hydrogen peroxide to oxidize a luminol system so as to produce the chemiluminiscence. The detection method comprises the following steps: forming a quadruplet G-DNA structure in a K+ and hemin solution system by adopting the DNA chain which is rich in G structure, wherein the quadruplet G-DNA structure has a peroxidase catalytic activity and can catalyze the hydrogen peroxide to oxidize the luminol system so as to produce the chemiluminiscence, the chemiluminiscence can be quenched when the naphthylamine compound exists in the system, and the detection of the naphthylamine can be realized according to the influence of the concentration variation on the quenching degree of the chemiluminiscence. The optical DNA biologic sensor has the advantages of simplicity in operation, short determination period, short response time, good stability, high sensitivity and the like.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and optical DNA biosensing, and relates to a method for detecting naphthylamine using an optical DNA biosensing method based on G-DNA peroxidase, which can quickly and effectively realize the detection of naphthalene amine in water. Naphthalene detection. Background technique [0002] Aromatic amines are an important class of environmental pollutants and have been listed as one of the priority environmental pollutants for monitoring. Aromatic amines are widely used in industry. As important chemical raw materials and fine chemical intermediates, they are often used in the production processes of printing and dyeing, pharmaceuticals, medicine, pesticides, plastics, rubber, textiles, ceramic glazing and paints, etc., especially In the printing and dyeing industry, wastewater often contains high concentrations of aromatic amine intermediates, and some dyes will eventually release aromat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/77
Inventor 刘新会梁刚巩文雯梁宝翠刘冠男成登苗
Owner BEIJING NORMAL UNIVERSITY