Methods and compositions for objectively characterizing medical images
A medical image, objective technology, applied in the direction of radiological diagnostic equipment, medical formula, image analysis, etc., can solve meaningless problems
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Embodiment 1
[0031] Embodiment 1-preparation double Gd liposome
[0032] A lipid mixture comprising DPPC, Gd-DTPA-BSA, cholesterol, and mPEG2000-DSPE in a molar ratio of 30:25:40:5 was dissolved in a chloroform:methanol (1:1 v / v) mixture. The solvent mixture was evaporated to dryness under vacuum and treated with gadobeglumine ( Gd-BOPTA, 500 mM Gd, Bracco Diagnostics Inc., Monroe Township, NJ) solution to hydrate the lipid content to achieve a lipid concentration of 40 mM. The solution was stirred at 60 °C for 90 min and then passed sequentially through 400 nm NUCLEPORE TM membrane Extruded five times through 200nm NUCLEPORE TM The film was extruded seven times and passed through a 100nm NUCLEPORE TM The film was extruded ten times. Using a molecular weight cut-off of 500kDa The resulting solution was diafiltered by a module (Spectrum Laboratories, RanchoDominguez, CA) to remove unencapsulated and unchelated Gd chelate molecules. Size analysis of the liposomes indicated that th...
Embodiment 2
[0033] Example 2 - MRI of mouse brain vasculature
[0034] Cerebral angiograms were obtained in C57BL / 6 mice intravenously injected with double Gd liposomes of Example 1 at a dose of 200 mg lipid / kg. Using Fast Spoilt Gradient (FSPGR) study using 512 3 Imaging was performed with the image matrix and the following parameters: repetition time (TR) = 20.0ms; echo time (TE) = 3ms; flip angle (FA) = 30°; field of view (FOV) = 30mm×30mm×30mm; Anisotropic images with a voxel size of 60 μ. figure 1 MRI image showing the vasculature of the mouse cranium. exist figure 1 In , the entire cranial vasculature is clearly visible, including the circle of Willis and microvessels around the 4th generation bifurcation through the carotid artery.
Embodiment 3
[0035] Example 3 - Morphometric Analysis
[0036] To test the sensitivity of the three morphometric techniques (multifractality, missingness, and two-dimensional autocorrelation), algorithms were implemented for each technique in MATLAB (MathWorks, Natick, MA). Use from figure 1 A two-dimensional section of the mouse brain vasculature, and shown in figure 2 middle. T1-weighted images with liposome contrast were windowed to show vascular signal but no soft tissue contrast. The section is at the right rostral process relative to the midline and includes vasculature in the thalamic and hippocampal sections. To test the sensitivity of morphometric measurements to the presence of vascular malformations, slice images were digitally processed to introduce subthreshold "holes". These holes are created when amyloid plaque deposits distort blood vessels. Three of the described cases were produced, one of which produced a single large hole of approximately 1mm x 0.5mm in the sect...
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