Nano microbial fertilizer as well as preparation method and application thereof
A technology of microbial fertilizers and nanomaterials, applied in the field of nano-microbial fertilizers, can solve problems such as improper use method or dosage, failure to achieve repair effect, poor lasting effect, etc. Fertilizer effect
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[0023] Preparation of biological strain spore powder
[0024] Biological strains include Brevibacillus lateralsporosa and Bacillus megaterium, and the preparation method and process of the spore powder of the two strains are the same. Wherein, the preparation of Brevibacillus lateralsporosa spore powder comprises the following steps:
[0025] Primary slant culture: inoculate Brevibacillus spp. on the slant of potato dextrose agar medium, and culture at 30°C for 24 hours to prepare the bacterial suspension;
[0026] Secondary seed culture: inoculate the prepared bacterial suspension into potato dextrose agar liquid medium, the inoculum amount is 1%, control the shaking table speed at 160 rpm to 200 rpm, at 25 ° C to 30 ° C Under culture 48h ~ 72h shaking culture to obtain seed liquid.
[0027] Preparation of spore powder: Spray the cultivated seed solution on the carrier and dry at a drying temperature lower than 80°C to obtain Brevibacillus lateralspora spore powder. The num...
Embodiment 1
[0029] Pour 0.2kg of Brevibacillus lateralspora spore powder; 0.2kg of Bacillus megaterium spore powder; 45kg of peat; 20kg of sepiolite; 30kg of organic fertilizer; Among them, the number of live bacteria of Brevibacillus lateralsporosa can be greater than 20 million / g, and the number of viable bacteria of Bacillus megaterium can be greater than 20 million / g.
Embodiment 2
[0031] Pour 0.5kg of Brevibacillus lateralsporosa spore powder; 0.5kg of Bacillus megaterium spore powder; 100kg of peat; 45kg of sepiolite; 45kg of organic fertilizer; Among them, the number of live bacteria of Brevibacillus lateralsporosa can be greater than 20 million / g, and the number of viable bacteria of Bacillus megaterium can be greater than 20 million / g.
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