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A method for producing Phellinus exopolysaccharide by submerged liquid fermentation

A technology of Phellinus Phellinus and Phellinus, which is applied in the field of microorganisms

Active Publication Date: 2016-08-24
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are a small number of researchers from universities and colleges at home and abroad who have conducted research on the submerged fermentation of Phellinus, but often the main focus is how to obtain higher biomass, and only a small number of researchers pay attention to the research on polysaccharides, the functional components of Phellinus.

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  • A method for producing Phellinus exopolysaccharide by submerged liquid fermentation
  • A method for producing Phellinus exopolysaccharide by submerged liquid fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for producing Phellinus exopolysaccharide by industrialized submerged liquid fermentation of the present invention is carried out according to the following steps:

[0030] 1. Preparation of Phellinus Phellinus Fermented Liquid: Inoculate the Phellinus Phellinus shake flask bacterial strain into seed medium (300L) by inoculum size 1%, wherein the seed medium consists of glucose 3%, silkworm chrysalis powder 0.4%, soybean cake powder 1.7%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, copper sulfate 0.2wt%, defoamer 0.02%, adjust the pH value to 6.5, the ventilation ratio is 1:0.8vvm, the tank pressure is 0.03Mpa, 26~28 Cultivate with shaking at ℃ for 5 days to obtain the Phellinus primary seed liquid; the primary seed liquid is inserted into the secondary seed medium (3000L) according to the inoculum size 15%, wherein the secondary seed medium consists of 3% glucose and 0.3% silkworm chrysalis powder. %, soybean cake powder 1.8%, ...

Embodiment 2

[0035] 1, the preparation of Phellinus Phellinus fermented liquid: inoculate the Phellinus Phellinus shake flask bacterial strain that activates in seed culture medium (300L) by inoculum size 1.5%, wherein seed culture medium consists of glucose 3%, silkworm chrysalis powder 0.3%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, copper sulfate 0.3wt%, defoamer 0.02%, adjust the pH value to 6.5, the ventilation ratio is 1:1.0vvm, the tank pressure is 0.03Mpa, 25~27 Cultivate with shaking at ℃ for 6 days to obtain Phellinus primary seed liquid; insert the primary seed liquid into the secondary seed medium (3000L) according to the inoculum size 15%, wherein the secondary seed medium consists of 3% glucose and 0.3% silkworm chrysalis powder %, soybean cake powder 2.0%, potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, copper sulfate 0.2wt%, defoamer 0.02%, adjust the pH value to 6.5, the ventilation ratio ...

Embodiment 3

[0040] In order to better verify the superiority of the composite prescription of short-acting glucose and long-acting starch in the industrial production of Phellinus polysaccharide, several comparative experiments were done. Only adjusted the consumption of carbon source glucose or (and) starch in the test in the liquid fermentation medium, do not contain starch in the comparative example 1, and glucose concentration is 1.5%; Do not contain glucose in the comparative example 2, and the starch concentration is 2.5%, to In ratio 3, the glucose content is 2.5%, and the starch content is 0.5%; all the other conditions are the same as in Example 1, and the test results are shown in Table 1:

[0041] Table 1

[0042]

[0043]

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Abstract

The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing Phellinus exopolysaccharide by submerged liquid fermentation. Specifically, it is a production method of Phellinus exopolysaccharide on an industrial scale. The method is to expand the activated Phellinus strains by inserting them into the seed tank, and then insert the expanded liquid seeds into the Fermentation is carried out in a special polysaccharide-producing liquid medium, and then the fermentation broth is separated to extract exopolysaccharides. The present invention studies the above-mentioned deficiencies in the prior art, on the basis of continuing to optimize the fermentation medium (especially the carbon source) and improving the biomass and exopolysaccharide in the small test and the pilot test, it solves the problem of scaling up from small test to industrialized production The difficulties encountered in the fermentation process parameter control, extraction equipment selection, optimization, etc., were finally successfully grafted into a 10‐20t fermenter for industrial production, and successfully produced high-quality Phellinus polysaccharides, truly realizing continuous, automated, Industrialized production.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing Phellinus exopolysaccharide by submerged liquid fermentation. Background technique [0002] Phellinus belongs to Basidiomycotlna, Hymenomucetes, PolyPorales, Hymenoechaetacae, Phellinus. Phellinus is a large genus of Polyporaceae. There are about 251 species recorded in the world, and 62 species are known in China. Phellinus is a precious medicinal fungus, known as "forest gold". "Shen Nong's Materia Medica" and ancient Chinese medical classics represented by Li Shizhen's "Compendium of Materia Medica" have "Sang Er", "Sang Huang", "Sang Chen", "Hu Sun Yan" and other descriptions, but for Sang Huang's definition should be said to be rather vague. [0003] In Japan, Phellinus Linteus (Phellinus Linteus), which has anti-cancer effects, is called "Mesimakobu", and it is written in Chinese characters as "Mesimakobu". . Since the research...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/645
Inventor 王文风徐国华徐玲王英燕张芙蓉杨亚威高岩许正宏
Owner JIANGSU SHENHUA PHARMA