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Breeding method of japonica rice variety capable of controlling postprandial blood sugar of diabetes patient

A technology for postprandial blood sugar and diabetic patients, which is applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve the problems of waste of manpower and material resources, low efficiency, and inability to solve the difficulty of eating for diabetics, and achieves accelerated breeding process, Good reproducibility, accurate and consistent results with phenotypic measurements

Inactive Publication Date: 2014-10-15
SHANGHAI ACAD OF AGRI SCI +1
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of improved, it can overcome the high resistance starch rice that can control the postprandial blood sugar of diabetics in the prior art, can't solve the difficult problem of diabetics' eating, meanwhile the efficiency of existing breeding method is low, Some shortcomings of wasting a lot of manpower and material resources

Method used

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  • Breeding method of japonica rice variety capable of controlling postprandial blood sugar of diabetes patient
  • Breeding method of japonica rice variety capable of controlling postprandial blood sugar of diabetes patient
  • Breeding method of japonica rice variety capable of controlling postprandial blood sugar of diabetes patient

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Embodiment 1

[0020] Example 1: The rice variety fibrin464 with high dietary fiber content excavated from immature immature embryos was treated with a chemical mutagen and the high-yield and high-quality rice line 007908 suitable for local ecological conditions was used as the male parent for hybridization, and the hybrid F1 generation was cultured for anthers. A genetically stable strain with high resistant starch content was obtained, and the field number was 08JD26. The japonica rice line 08JD26 with high resistant starch content was used as the female parent, and it was crossed with the indica rice variety Miyang 23. The offspring of the hybrids were self-crossed, and there was a significant correlation between the opaque grains of the F1 generation seeds and the content of resistant starch, and the offspring individuals with transparent grains No single plant with high resistant starch content appeared in the population, and the occurrence rate of high resistant starch content in the of...

Embodiment 2

[0026] Example 2: Screening using molecular markers closely linked to high resistant starch content;

[0027] DNA was extracted from the test samples using the CTAB method. Cut a small piece of leaf 4-5cm at the early stage of rice tillering, add 700μL 1.5×CTAB (containing 1.5%CTAB, 75mM Tris-HCl, 15mM EDTA, 1.05M NaCl), and grind thoroughly; 2) Transfer the homogenate into 1.5ml Centrifuge tube, cool to room temperature in a water bath at 56°C for 20 minutes; 3) Add an equal volume of chloroform:isoamyl alcohol (24:1) and shake well; 4) Centrifuge at the highest speed (13200rpm) for 10 minutes; 5) Transfer the supernatant to Create a new centrifuge tube, add twice the volume of pre-cooled 100% ethanol, and centrifuge to collect DNA after standing still for 20 minutes; 6) Remove the supernatant, air-dry the DNA, add 50-100 μL of double distilled water to dissolve, and detect in a UV spectrophotometer . Dilute the DNA to prepare a set of DNA working solution with a concentrat...

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Abstract

The invention relates to a breeding method of a japonica rice variety capable of controlling postprandial blood sugar of diabetes patients and belongs to the field of breeding of a new variety of rice. A japonica rice variety 'sugar-optimized rice No.1', in which content of resistant starch is higher than 10%, is bred in a manner of employing chalky rate, the content of amylose and the content of the resistant stratch and combining with methods such as molecular marker-assisted selection and the like. Yield of the 'sugar-optimized rice No.1' is higher than that of a 08JD26 strain and is 405 kg / mu. The invention belongs to the breeding method of a japonica rice variety in which the resistant starch is high in content. The method is used for breeding of the japonica rice variety which is suitable for the diabetes patients.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for breeding japonica rice varieties for controlling postprandial blood sugar in diabetics. Background technique [0002] In the past 20 years, with the development of social economy and the increase and aging of the population, the incidence of diabetes has risen rapidly, and it is becoming younger, and it has gradually become a chronic non-communicable disease that is prevalent in the world. At present, the number of diabetic patients in my country has exceeded 92 million, and the incidence rate is rising rapidly at 9.9% per year, and it is showing a younger trend, ranking first in the world, while the annual direct medical expenses for the treatment of type 2 diabetes and its complications have reached More than 18.75 billion yuan has caused serious economic burden and social pressure. [0003] Resistant starch belongs to polysaccharides and has no energy. It ca...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 朴钟泽白建江杨瑞芳楼素芬
Owner SHANGHAI ACAD OF AGRI SCI
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