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Microfluidic chip-based pressurized cell culture system and method

A microfluidic chip, cell culture technology, applied in the field of cell biology and cell mechanics, can solve the problems of difficult control of pressure and operability to be improved, so as to reduce interference, save analysis time and cost, and facilitate the cell microenvironment. Effect

Active Publication Date: 2016-06-01
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operability of this method needs to be improved, and the pressure is not easy to control
[0004] At present, microfluidic chips and systems containing microfluidic chips have been widely used in many technical fields, especially in the field of cell biology technology, while in related technical fields such as simulating high blood pressure, it is necessary to apply a certain pressure during the cell culture process. Close to the physiological environment in vivo, so far there has been no report on the microfluidic chip culture system with pressurized operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Microfluidic chip-based pressurized cell culture system and method

[0031] Such as figure 1 As shown, the microfluidic chip-based pressurized cell culture system includes a syringe pump, a microfluidic chip and a pressure gauge, the syringe pump and the pressure gauge are respectively connected to the microfluidic chip through flexible tubes, and the syringe pump and the pressure gauge are respectively connected to the microfluidic chip. Pressure gauges are located on both sides of the microfluidic chip. When the pressurized cell culture system of this connection mode is used, the cells cultured in the channel of the microfluidic chip bear both pressure and flow shear force.

[0032] The microfluidic chip is made of PDMS and glass, and has a straight channel chip structure, in which there is only one channel, which is 3 cm long, 2 mm wide, and 200 μm high.

[0033] First, the cells were seeded into the channel, and after the cells adhered to the wall and gr...

Embodiment 2

[0034] Example 2 Microfluidic chip-based pressurized cell culture system and method

[0035] Such as figure 2 As shown, wherein the microfluidic chip-based pressurized cell culture system includes a syringe pump, a microfluidic chip and a pressure gauge, and the syringe pump and the pressure gauge are respectively connected to two of the three interfaces of a T-joint, The other interface of the T-shaped joint is connected to one end of the channel in the microfluidic chip, the other end of the channel is connected to a flexible tube, and the other end of the flexible tube is clamped by a clip to make it closed. When using the T-joint pressurized cell culture system to culture cells, the cells in the channel of the microfluidic chip only bear pressure.

[0036] The microfluidic chip is made of PDMS and glass, with multiple parallel channels, each channel is 2cm long, 1mm wide, and 75μm high, such as image 3 shown.

[0037] First, the cells were seeded into the channel, and a...

Embodiment 3

[0038] Example 3 Microfluidic chip-based pressurized cell culture system and method

[0039] Such as figure 1 As shown, the microfluidic chip-based pressurized cell culture system includes a syringe pump, a microfluidic chip and a pressure gauge, the syringe pump and the pressure gauge are respectively connected to the microfluidic chip through flexible tubes, and the syringe pump and the pressure gauge are respectively connected to the microfluidic chip. Pressure gauges are located on both sides of the microfluidic chip.

[0040] The microfluidic chip is made of PDMS and glass, and has a meandering channel chip structure, in which there is only one channel, which is 100 μm wide and 20 μm high, such as Figure 4 shown.

[0041] First, the cells were seeded into the channel, and after the cells adhered to the wall and grew to about 80% confluence, the pressure test was performed. When the cell culture system is used for pressure experiments, the syringe pump pushes the syrin...

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Abstract

The invention provides a micro-fluidic chip-based pressurized cell culturing system and a method thereof. The system comprises a micro-fluidic chip, a pressure driving device, connecting pipelines and a pressure detecting device, the micro-fluidic chip contains a channel, the pressure driving device comprises a container used for containing a medium and matched with the pressure driving device, the container is connected with the micro-fluidic chip through one of the connecting pipelines, the pressure driving device pushes the medium in the container to enter the micro-fluidic chip, and the pressure detecting device is connected with the micro-fluidic chip through one of the connecting pipelines. The system can conveniently culture cells under the action of shearing force and a pressure and realizes relevant researches, and the pressurized cell culturing system is convenient to dismount and carry.

Description

technical field [0001] The invention relates to the technical fields of cell biology and cell mechanics, in particular to a microfluidic chip-based cell pressurized culture system and method. Background technique [0002] In 1990, Swiss scientists Manz and Widmer proposed the concept of Micrototal analysis system (Micrototal analysis system), whose goal is to integrate the functions of the entire analytical laboratory into a portable device or even a tiny chip. Therefore, the micro-total analysis system is also called "Lab-on-a-chip (LOC)". The microfluidic chip (Microfluidic chip) is an important part of the LOC, and its main feature is that the effective structure containing the fluid (including channels, reactors and other functional units) is micron-scale in at least one dimension. Based on micro-electromechanical processing technology, microfluidic chips manufacture channels on silicon wafers, glass or PDMS and integrate drive and inspection devices. Through the contro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/04C12M1/34C12N5/00
CPCC12M23/16C12M35/04C12M41/40
Inventor 李雷陈弘达刘静施雪涛
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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