Linear scanning-based fluorescence confocal detection device

Abstract

The invention discloses a linear scanning-based fluorescence confocal detection device. The linear scanning-based fluorescence confocal detection device is characterized in that a dichroic prism is arranged in an optical path of an exciting light beam and forms an angle of 135 degrees with the optical path, a rectangular prism is arranged in an optical path of the exciting light transmitted by the dichroic prism and forms an angle of 45 degrees with the optical path, the transmission light is reflected to an object lens by the rectangular prism and then is focused to a sample so that an optical signal is obtained, the optical signal goes through the object lens, then is orderly reflected by the rectangular prism and the dichroic prism, then orderly goes through an optical lens I, a pinhole and an optical lens II and then is parallelly transmitted to an optical detector so that an optical signal, a spectrum signal or an optical intensity signal of the sample is obtained, and the rectangular prism and the object lens can carry out rectilinear motion along an exciting light incident direction. Through combination of the traditional confocal system and a scanning platform, the confocal system can realize multichannel scanning of a lot of samples. The linear motor scanning platform carries out scanning so that detection frequency and a detection precision are improved.

Description

technical field [0001] The invention relates to a fluorescence confocal detection device based on linear scanning. Background technique [0002] Since the beginning of the 21st century, biochip technology has developed rapidly, and its high-throughput, miniaturization, and automation characteristics have put forward relatively high requirements for related supporting instruments. The confocal optical system means that the excitation light is focused on the sample surface while the emitted light is concentrated on the pinhole, and the focus depth of the system on the sample surface is limited by the pinhole to reduce the signal intensity of the background light, thereby greatly improving the signal-to-noise ratio of the system and detection limit. General confocal scanning microscopes use gratings to expand the converging range of excitation light to image samples in the field of view. However, limited by the size of the objective lens and pinholes, the detection range is sm...

AI Technical Summary

Problems solved by technology

Since its imaging is aimed at the entire sample surface, high-throughput detection can be performed, but the limitations of this detection method are also obvious: 1. It is not a confocal system, the signal-to-noise ratio is not ideal, and the detection limit is low; 2. Since the entire plane is imaged, points without fluorescent signals on the plane will have more stray light interference detection; 3. Some software is needed to analyze the collected images and extract signal points, and the software calculation is limited by computer speed , so there is a certain limitation in the timeliness of detection

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