Centrifugal sleeve tube structure used for separating and extracting DNA

Abstract

A centrifugal sleeve tube structure used for separating and extracting DNA belongs to the field of equipment used for separation and purification of nucleic acid DNA or RNA. The structure includes a centrifugal tube. A connecting ribbon is formed on an upper part of the centrifugal tube. A plug cover is connected to an end of the connecting ribbon. An inner sleeve tube is disposed in a centrifugal tube chamber. A liquid discharge groove is formed on a bottom wall of an inner sleeve tube chamber and one side, which is opposite to the inner sleeve tube chamber, of the bottom wall is extended to form an inner sleeve connecting port. An impurity-filtering mechanism includes a transition sleeve cylinder and a coil. A transition sleeve cylinder matching joint is formed on a central position of the bottom of a transition sleeve cylinder chamber, wherein the center of the joint forms a liquid discharge hole. The coil is disposed in the transition sleeve cylinder chamber with one end being matched with the inner sleeve connecting port and the other end being matched with the transition sleeve cylinder matching joint. A coil chamber of the coil is filled with filtering materials. By means of the structure, a secondary pipetting process is unnecessary so that a water-soluble DNA template is free from losing; and an impurity-removing effect is excellent and a PRC amplification operation is ensured. The structure is convenient, quick to operate, is labor-saving, is good in the impurity-removing effect, is simple and is easy to manufacture.

Description

technical field [0001] The invention belongs to the technical field of equipment for separating and purifying nucleic acid DNA or RNA, and in particular relates to a centrifuge sleeve structure for separating and extracting DNA. Background technique [0002] Separation and purification, that is, extraction of DNA, has positive significance in the fields of medical treatment, criminal case investigation, identification of victims of various accidents such as traffic accidents, archaeology, and animal and plant inspection and quarantine. DNA detection methods are mainly but not important. It is limited to the following types: phenol-chloroform extraction method, chelex-100 method; magnetic bead method; organic solvent method; salting-out method and silica bead method, etc. However, no matter which method is used, its common characteristics or common requirements can be roughly summarized into the following two aspects: one is to maximize the yield and purity of DNA or RNA and ...

AI Technical Summary

Problems solved by technology

For this reason, the applicant has searched the literature, and the Chinese patent authorization announcement number CN201744365U recommends a "filter membrane centrifuge tube". It can be seen from the description that there is no need to pipette during the operation, but due to the unreasonable structure, there are the following disadvantages: First, impurities are easy to enter the lower part of the centrifuge tube from the filter membrane (the patent is called a mixed fiber microporous filter membrane) with the body fluid, thus affecting the water-soluble The purity of the DNA template is affected; the second is that it is difficult to form an ideal seal between the rubber ring for the filter membrane and the inner wall of the centrifuge tube, so impurities will also enter from the micro-gap between the rubber ring and the inner wall of the centrifuge tube The lower part of the centrifuge tube, because the rubber ring taught by the patent must meet the requirements of being pluggable with the lumen wall of the centrifuge tube, so if in order to achieve the ultimate sealing effect between the rubber ring and the lumen wall of the centrifuge tube, Then it is difficult to remove the rubber ring together with the pressure ring and the filter membrane from the centrifuge tube through the hanging ring on the rubber ring in the subsequent operation steps. On the contrary, if the rubber ring is taken out in order to ensure convenience, it will inevitably affect the lumen of the rubber ring and the centrifuge tube. The sealing effect between the cavity walls; the third is that only one filter membrane cannot objectively reflect the good effect of filtering out impurities, thus affecting PCR amplification

[0006] Authorized announcement number CN202610231U provides a "multi-layer nucleic acid adsorption centrifuge sleeve". Because the principle of DNA extraction in this patent solution is opposite to that of the aforementioned patent, that is, the adsorption method is used. Although there is no need to pipette during the operation, the DNA template is lost. Difficult to control, please refer to column 0025 of the patent specification for details

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