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Solid fermentation method of cordyceps sinensis

A technology of Cordyceps sinensis and solid fermentation, applied in the field of bioengineering, can solve the problems of mycelium damage, injury, short mycelium, etc., and achieve the effect of reducing losses

Inactive Publication Date: 2014-11-05
GUIZHOU QIAN KUN BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the submerged fermentation process of Cordyceps sinensis mycelium, because of the stirring and turning of the fermentation system, the destruction and damage to the mycelium are caused. will be lost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the present invention is a kind of solid fermentation method of Cordyceps sinensis, it is characterized in that comprising the following steps:

[0017] A, the Cordyceps sinensis seed fermented bacterium liquid that expands culture, inoculate on the fermented medium in the fermenter and cultivate, the volume of the Cordyceps sinensis seed bacterium liquid is 10-15% of the fermented liquid volume in the fermenter, and its cultivation condition is: temperature 15-20°C, shaker speed 100-120r / min, pH 5.5-6, tank pressure 0.05-0.1MPa, ventilation volume 0.5-1.0VVm, culture time 60-72 hours;

[0018] The composition of the fermentation medium is calculated by mass: glucose is 2.0%, peptone is 4.0%, KH 2 PO 4 0.20%, MgSO 4 0.10% for corn syrup, 0.15% for corn steep liquor, 0.25% for molasses, and the rest is water;

[0019] B. Sampling inspection. At this time, the mycelium grows vigorously and has a uniform size. The sampled bacterial liquid is separated from...

Embodiment 2

[0021] Example 2, the difference between Example 2 and Example 1 is that the culture conditions of the Cordyceps sinensis seed fermentation bacterial liquid are: temperature 18-20°C, shaker speed 110r / min, PH6, tank pressure 0.05-0.1MPa, ventilation The amount is 0.8VVm, and the culture time is 60-72 hours.

Embodiment 3

[0022] Embodiment 3, a kind of solid fermentation method of Cordyceps sinensis is characterized in that comprising the following steps:

[0023] 1), use biological polysaccharides to thicken, and culture mycelium solidly;

[0024] A, the Cordyceps sinensis seed fermentation bacterium liquid that expands cultivation, inoculate on the fermentation medium in the fermenter and cultivate, the volume of the Cordyceps sinensis seed bacterium liquid is 12% of the fermented liquid volume in the fermenter, and its culture condition is: temperature 20 ℃ , Shaker speed 120r / min, PH5.5, tank pressure 0.08MPa, ventilation volume 1.0VVm, culture time 60 hours;

[0025] The composition of the fermentation medium calculated by mass is: 2.0% of glucose, 4.0% of peptone, 0.20% of KH2PO4, 0.10% of MgSO4, 0.15% of corn steep liquor, 0.25% of molasses, and the rest is water;

[0026] B. Sampling inspection. At this time, the mycelium grows vigorously and has a uniform size. The sampled bacterial l...

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Abstract

The invention relates to a solid fermentation method of cordyceps sinensis, which belongs to the technical field of bioengineering. The method comprises the following steps: A)fermenting a bacteria liquid by cultured cordyceps sinensis seeds, inoculating to a fermentation medium in a fermentation cylinder for culturing; B)sampling and examining, separating thalline from the sampled bacteria liquid through a high speed centrifuge, when the content of 100mL of moisture thalline of a fermentation broth is greater than 9 grams, adding sterilized sterilization accessories in the fermentation cylinder, uniformly stirring the fermentation broth, thickening to form a paste colloid containing cordyceps sinensis mycelia, uniformly distributing the paste colloid on a sterilized culture bed in a solid fermentation house for solid culture; and C)sampling and examining, when diameter of mycelia reaches about 20mum, diameter of spore reaches about 12 mum, and then completing fermentation. The method can effectively avoid damage due to continuous stirring during a liquid culture process of the mycelia; mycelia spore is difficultly lost, so that the cordyceps sinensis mycelia product with high quality and high yield can be obtained.

Description

technical field [0001] The invention relates to a solid fermentation method of Cordyceps sinensis, belonging to the technical field of bioengineering. Background technique [0002] Cordyceps sinensis is a national second-class protected plant. Mainly distributed in the alpine meadows of Tibet, Qinghai, Sichuan, Yunnan and Gansu provinces (regions), it has the effects of nourishing the kidneys and lungs, nourishing the heart and liver, and enhancing disease resistance. [0003] In recent years, domestic and foreign market demand has doubled, and prices have soared, resulting in artificial over-excavation, severe damage to Cordyceps resources, and the market is in short supply. The Cordyceps sinensis mycelium produced by submerged fermentation has similar ingredients and similar pharmacological effects to natural Cordyceps. At present, the submerged liquid fermentation process is generally used in factories. [0004] However, in the submerged fermentation process of Cordycep...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 史兰东藤国霞
Owner GUIZHOU QIAN KUN BIOPHARM
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