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Anti medroxyprogesterone specific monoclonal antibody hybridoma cell strain and application thereof

A hybridoma cell line, monoclonal antibody technology, applied in biochemical equipment and methods, microorganisms, microorganism-based methods, etc., can solve problems such as difficulty in obtaining accurate and objective results, achieve good application value, simple synthesis process, High sensitivity effect

Active Publication Date: 2014-11-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current MP immunoassay method, the key is the high specificity and high sensitivity of the monoclonal antibody to MP, so that it is difficult to obtain accurate and objective results

Method used

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  • Anti medroxyprogesterone specific monoclonal antibody hybridoma cell strain and application thereof
  • Anti medroxyprogesterone specific monoclonal antibody hybridoma cell strain and application thereof
  • Anti medroxyprogesterone specific monoclonal antibody hybridoma cell strain and application thereof

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Experimental program
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Embodiment 1

[0029] (1) Preparation of medroxyprogesterone hapten: 34.4 mg (0.1 mmol) of medroxyprogesterone was dissolved in 3 mL of anhydrous dimethyl sulfoxide to obtain liquid A, and 27.8 mg (0.2 mmol) of dibromoacetic acid was dissolved in 1.4 mL In anhydrous dimethyl sulfoxide, obtain liquid B, add potassium hydroxide powder 208.5 mg (1.5 mmol) to liquid A, stir at room temperature until the color of the solution turns slightly yellow, add liquid B dropwise to liquid A, The yellow color gradually deepened, reacted at room temperature for 4 hours, terminated the reaction with 50 mL of ice water, extracted three times with ethyl acetate, combined the water phase, acidified the water phase with 2 mol / L hydrochloric acid to obtain a yellow precipitate, washed four times with water until neutral, and dried to obtain a yellow color Medroxyprogesterone Hapten (MP-2CME) Powder.

[0030] The product was identified by LC-MS after dissolving in methanol.

[0031] (2) Preparation of Medroxyprog...

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Abstract

The invention discloses an anti medroxyprogesterone specific monoclonal antibody hybridoma cell strain and application thereof, and belongs to the technical field of food safety immune detection. The preservation number of monoclonal cell line E# hybridoma cell strain 4F9 is CGMCC No.9305. The cell strain can secrete and produce monoclonal antibodies against medroxyprogesterone, has good specificity and high sensitivity, the medroxyprogesterone 50% inhibitory concentration IC50 is 0.067ng / mL, and the cross reaction rate with other analogues is less than 0.1%. An immune detection kit can be prepared by use of the monoclonal antibodies against the medroxyprogesterone. The antibodies produced by use of the cell strain can be used for multiple purposes, and can provide powerful testing tools and means for guaranteeing the rapid and healthy development of animal husbandry and food safety.

Description

technical field [0001] The invention relates to a hybridoma cell line and the application thereof for producing anti-medroxyprogesterone specific monoclonal antibody, and belongs to the technical field of food safety immune detection. Background technique [0002] Medroxyprogesterone (Medroxyprogesterone, MP) is a progestin, similar to natural progesterone, and is a synthetic progesterone derivative, so it is also called medroxyprogesterone. In terms of medicine, it is mainly used for dysmenorrhea, functional amenorrhea, habitual abortion, etc. Large doses can be used as long-acting contraceptive injections. In terms of animal husbandry, it is mainly used to promote the growth of animals, but long-term large-scale use will cause harm to animals. At present, many countries prohibit the use of hormones in raising animals, and my country has also issued corresponding policies to prohibit the use of hormones. Existing MP detection methods include high performance liquid chroma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/20C12P21/08C12R1/91
Inventor 匡华孔娜胥传来徐丽广马伟刘丽强宋珊珊吴晓玲
Owner JIANGNAN UNIV