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Magnetic-targeted isoniazid composite drug carrying microspheres and preparation method thereof

A technology of drug-loaded microspheres and isoniazid, which is applied in the field of medicine, can solve problems such as toxic and side effects, and achieve the effects of low toxicity, high drug loading, and reduced drug resistance

Inactive Publication Date: 2014-11-19
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN201210470282.2 discloses a method for preparing a rapamycin / magnetic carboxymethyl chitosan nanometer drug-loaded microsphere by emulsification and cross-linking. Although the drug-loaded microsphere prepared by this method has strong targeting and slow release Good performance and small particle size, but a large amount of toxic reagents are used in the preparation process, which may cause toxic and side effects

Method used

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  • Magnetic-targeted isoniazid composite drug carrying microspheres and preparation method thereof
  • Magnetic-targeted isoniazid composite drug carrying microspheres and preparation method thereof
  • Magnetic-targeted isoniazid composite drug carrying microspheres and preparation method thereof

Examples

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Embodiment 1

[0027] Accurately weigh 2.97g of ferric chloride hexahydrate, 1.529g of ferrous sulfate heptahydrate, dissolve in 50 mL of deionized water, stir to dissolve, add to a three-necked flask, seal, stir, and pass through N 2, heated to 80°C, then slowly added 10 ml of ammonia water, continued to stir, reacted for 30 minutes, cooled to room temperature, washed three times with deionized water, washed three times with ethanol, magnetically separated and freeze-dried to obtain magnetic Fe 3 o 4 . Accurately weigh 0.03g of Fe 3 o 4 Add to the chitosan solution with a concentration of 2 mg / mL, add 0.1 g of isoniazid, stir, add dropwise a sodium tripolyphosphate solution with a concentration of 1.5 mg / mL, stir for 10 min, wash with deionized water three times, and magnetically separate After freeze-drying, Fe 3 o 4 / CS / INH composite drug-loaded microspheres. Transmission electron microscope observation (see figure 2 ), as shown in TEM figure 2 As shown, the results show that Fe...

Embodiment 2

[0029] Accurately weigh 4.444g of ferric nitrate nonahydrate, 1.095g of ferrous chloride tetrahydrate, dissolve in 50mL deionized water, stir to dissolve, add to a three-necked flask, seal, stir, pass in argon, heat to 80°C and then slowly add 10 ml of ammonia water, keep stirring, react for 30min, cool to room temperature, wash three times with deionized water, wash three times with ethanol, freeze-dry after magnetic separation, and obtain magnetic Fe 3 o 4 . Accurately weigh 0.03g of Fe 3 o 4 Add to the chitosan solution with a concentration of 4 mg / mL, add 0.1 g of isoniazid, stir, add dropwise a sodium tripolyphosphate solution with a concentration of 3 mg / mL, stir for 20 min, wash with deionized water three times, and magnetically separate freeze-dried to obtain Fe 3 o 4 / CS / INH composite drug-loaded microspheres. Measuring Fe under Superconducting Quantum Magnetometer 3 o 4 and Fe 3 o 4 The magnetic size of / CS / INH composite drug-loaded microspheres (see imag...

Embodiment 3

[0031] Accurately weigh NH 4 Fe(SO 4 ) 2 2.948g, (NH 4 ) 2 Fe(SO 4 ) 2 Dissolve 1.562 g in 50 mL deionized water, stir to dissolve, add to a three-necked flask, seal, stir, pass in argon, heat to 90°C, then slowly add 10 ml of NaOH, keep stirring, react for 60 minutes, cool to room temperature, and use Wash three times with deionized water, wash three times with ethanol, and freeze-dry after magnetic separation to obtain magnetic Fe 3 o 4 . Accurately weigh 0.03g of Fe 3 o 4 Add to the chitosan solution with a concentration of 6 mg / mL, add 0.1 g of isoniazid, stir, add dropwise a sodium tripolyphosphate solution with a concentration of 5 mg / mL, stir for 60 min, wash with deionized water three times, and magnetically separate freeze-dried to obtain Fe 3 o 4 / CS / INH composite drug-loaded microspheres. Fe 3 o 4 , INH, CS and Fe 3 o 4 The infrared spectrum of / CS / INH composite drug-loaded microspheres (see Figure 4 ). The results showed that Fe 3 o 4 / CS / IN...

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Abstract

The invention discloses magnetic-targeted isoniazid composite drug carrying microspheres and a preparation method thereof. The composite drug carrying microspheres comprise the following components: 1-89% of magnetic Fe3O4, 1-80% of isoniazid and 10-19% of chitosan. The preparation method comprises the following steps: (1) preparing the magnetic Fe3O4, namely dissolving a Fe<3+>-containing compound and a Fe<2+>-containing compound in water, introducing inert gas, adding an alkali solution and stirring at a certain temperature to obtain the magnetic Fe3O4; and (2) preparing the composite drug carrying microspheres, namely adding the Fe3O4 and the isoniazid into an acid solution of the chitosan, dripping a sodium tripolyphosphate solution at a certain temperature, and carrying out magnetic separation and freeze drying to obtain the magnetic-targeted isoniazid composite drug carrying microspheres. The magnetic-targeted isoniazid composite drug carrying microspheres disclosed by the invention have the advantages of superparamagnetism, small particle size, dispersion uniformity, good biocompatibility and the like and can be used for magnetic-guided passive targeting therapy.

Description

technical field [0001] The invention relates to an isoniazid drug-loading system, in particular to a magnetic-targeted isoniazid composite drug-loaded microsphere and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Magnetic nanoparticles have some special properties, such as high coercive force and superparamagnetism, so they can be used as carriers to carry drugs in the field of biomedicine, so that the drugs can be targeted, thereby improving the utilization rate of drugs and reducing toxic and side effects. Nano Fe 3 o 4 It is widely used because of its small particle size, good biocompatibility, high sensitivity, strong magnetic response, low toxicity, stable properties, and easy availability of raw materials. [0003] Isoniazid (INH) is the first-line drug for the treatment of tuberculosis, and it is a small molecule water-soluble drug. Isoniazid is highly selective to Mycobacterium tuberculosis and has strong...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K31/4409A61K47/36A61K41/00A61P31/06
Inventor 马振叶张侠秦红王春梅张亮
Owner NANJING NORMAL UNIVERSITY
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