Ultraviolet mutagenesis type pseudomonas florescens and application thereof

A Pseudomonas fluorescens, application technology, applied in the field of microorganisms, can solve the problems of Pseudomonas fluorescens single biocontrol function, single phosphorus-solubilizing effect, etc.

Active Publication Date: 2014-11-19
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The reported Pseudomonas fluorescens has a single biocontrol function or a single phosphorus-solubilizing effect, and Pseudomonas fluorescens has not yet been reported.

Method used

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  • Ultraviolet mutagenesis type pseudomonas florescens and application thereof
  • Ultraviolet mutagenesis type pseudomonas florescens and application thereof
  • Ultraviolet mutagenesis type pseudomonas florescens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: Isolation, purification and identification of ultraviolet mutagenized Pseudomonas fluorescens F1

[0080] The implementation steps of this embodiment are as follows

[0081] A. Separation

[0082] Select a decaying soil plot with wheat take-all in Zhoukou City, Henan Province, take 100 g of soil from the periphery and the center of the plot within a depth of 10-20 cm, and mix them in equal amounts.

[0083] Dissolve 10g of soil sample in 90ml of water, shake and cultivate for 20min in the QHZ-98A full-temperature shaking incubator produced by Taicang Huamei Biochemical Instrument Factory, and obtain the supernatant according to the dilution of 10 -3 、10 -4 、10 -5 For gradient dilution, 100 μl of the diluted solution was coated on 10 g / L glucose, 0.5 g / L (NH 4 ) 2 SO 4 , 0.3g / L MgSO 4 ·7H 2 O, 0.3g / L NaCl, 0.3g / L KCl, 0.03g / L FeSO 4 ·7H 2 O, 0.03g / L MnSO 4 4H 2 O, 10.0g / L Ca 3 (PO 4 ) 2 On the Montina inorganic phosphorus solid medium (pH7.0-7.5...

Embodiment 2

[0096] Example 2: Determination of Phosphorus Solubilizing Ability of Wild-type Pseudomonas fluorescens F0

[0097] Inoculate the wild-type Pseudomonas fluorescens F0 preserved on the slant medium into conventional LB liquid medium, and cultivate it to the logarithmic phase at a temperature of 30°C and a rotation speed of 180rpm, according to the volume of Montina inorganic phosphorus medium Inoculate 1% of the inoculum amount of the wild-type Pseudomonas fluorescens F0 into 100ml of sterilized Montina inorganic phosphate medium, shake and cultivate for 6 hours under the conditions of temperature 28°C and rotational speed 180rpm. Take 5ml shaking culture solution and centrifuge for 5min under the conditions of rotating speed 12000rpm and temperature 4°C, take 0.1ml supernatant and dilute to 5ml in a graduated test tube, then add 1ml constant volume solution to a 10ml colorimetric tube, and then add 2ml molybdenum-antimony anti-color developer, dilute to the scale with water, p...

Embodiment 3

[0100] Embodiment 3: The ultraviolet mutagenesis test of wild-type Pseudomonas fluorescens

[0101] A. Pick the single colony F0 of wild-type Pseudomonas fluorescens isolated from the decayed soil of wheat take-all, and insert it into NB composed of 5g / L beef extract, 10g / L peptone, and 5g / L sodium chloride In the culture medium (pH7.0-7.2), cultivate at a temperature of 28-30°C and a rotation speed of 180rpm for 16-20 hours; take 2ml of the culture solution and put it in a sterile petri dish with a diameter of 75mm, and then place it in a UV light with a power of 20W. Under the lamp, irradiate for 90s at a distance of 30cm from the ultraviolet lamp, and then dilute the bacterial solution to 10 in the dark. -6 , and then coated with 10.0g / L glucose, 0.5g / L (NH 4 ) 2 SO 4 , 0.3g / L MgSO 4 ·7H 2 O, 0.3g / L NaCl, 0.3g / L KCl, 0.03g / L FeSO 4 ·7H 2 O, 0.03g / L MnSO 4 4H 2 O, 10.0g / L Ca 3 (PO 4 ) 2 On the Montina inorganic phosphorus solid medium (pH7.0~7.5) composed of 20.0...

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Abstract

The invention discloses an ultraviolet mutagenesis type pseudomonas florescens F1 having the effects of dissolving potassium and biologically controlling a number of soil-borne diseases. The control effect of the wheat take-all of the ultraviolet mutagenesis type pseudomonas florescens F1 is up to 85-100%, the control effect of the pepper bacterial wilt is up to 70-90%, and the phosphorus dissolving effect is up to 480-552mg/L, which is 40% higher than that of a wild strain.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the present invention relates to a strain of ultraviolet mutagenic Pseudomonas florescens (P.florescens) F1 and its application. 【Background technique】 [0002] Phosphorus is one of the essential nutrients for plant growth and development, and participates in photosynthesis and biochemical reactions in plants. Phosphorus has no stable gaseous compounds in the atmosphere, and its cycle is a typical deposition cycle. The phosphorus used by plants mainly comes from the soil. The total phosphorus content of the soil plow layer in my country is 0.4-2.5g / kg, but the available phosphorus available to plants is less than 1% of the total phosphorus. Phosphorus-dissolving microorganisms are widely distributed in soil and play a central role in the natural phosphorus cycle. Phosphorus-dissolving microorganisms can convert insoluble phosphorus into soluble phosphorus for plant g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C09K17/00C12R1/39C09K101/00
Inventor 杨娜李丽艳葛振宇朱瑞艳杜迎辉徐志文
Owner 领先生物农业股份有限公司
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