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Method for preparing nanometer iron citrate liposome

A technology of ferric citrate and citric acid, applied in the field of biomedicine, can solve the problems of small particle size of ferric citrate liposome, poor brain targeting, and achieve the effects of regular particle size, high encapsulation rate and good stability

Inactive Publication Date: 2014-11-26
HEBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing nanometer ferric citrate liposomes, the ferric citrate liposomes obtained by the method are small in particle size, good in stability, have the characteristics of long-acting sustained release, and can be administered through the nasal cavity , easy to cross the blood-brain barrier, which solves the defect of poor brain targeting of conventional iron supplementation preparations

Method used

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  • Method for preparing nanometer iron citrate liposome
  • Method for preparing nanometer iron citrate liposome
  • Method for preparing nanometer iron citrate liposome

Examples

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Effect test

Embodiment 1

[0025] Example 1 A method for preparing nano-ferric citrate liposomes (encapsulation efficiency 92.33%)

[0026] Weigh soybean lecithin and cholesterol according to mass ratio = 8:1, dissolve in absolute ethanol; weigh iron citrate according to mass ratio iron citrate: soybean lecithin = 1:6, dissolve with PBS (pH =8.0) . Take a certain volume of ferric citrate solution in a 100ml beaker, place it in a water bath at 40~60°C, add the suspension of lecithin and cholesterol dropwise while stirring, and continue stirring until the ethanol is completely volatilized. Sonicate the liposome suspension in a water bath for 10 min to obtain a milky yellow translucent liposome suspension, dialyze for 4-5 hours in the dark, filter with a filter membrane, the encapsulation rate is 92.33%, and then place it in a brown bottle and seal it tightly , stored in the refrigerator at 4°C, and set aside.

Embodiment 2

[0027] Example 2 A method for preparing nano-ferric citrate liposomes (encapsulation efficiency 82.16%)

[0028] Weigh soybean lecithin and cholesterol according to mass ratio = 4:1, dissolve in absolute ethanol; weigh iron citrate according to ferric citrate: soybean lecithin = 1:8, dissolve with PBS (pH = 8.0). Take a certain volume of ferric citrate solution in a 100ml beaker, place it in a water bath at 40~60°C, add the suspension of lecithin and cholesterol dropwise while stirring, and continue stirring until the ethanol is completely volatilized. Sonicate the liposome suspension in a water bath for 30 min to obtain a milky yellow translucent liposome suspension, dialyze for 4-5 hours in the dark, filter with a filter membrane, and the encapsulation rate is 82.16%, then place it in a brown bottle and seal it tightly , stored in the refrigerator at 4°C, and set aside.

Embodiment 3

[0029] Example 3 A method for preparing nano-ferric citrate liposomes (encapsulation efficiency 80.80%)

[0030] Weigh soybean lecithin and cholesterol according to mass ratio = 8:1, and dissolve in absolute ethanol; according to ferric citrate: soybean lecithin = 1:8, weigh ferric citrate, and dissolve with PBS (pH = 6.5). Take a certain volume of ferric citrate solution in a 100ml beaker, place it in a water bath at 40~60°C, add the suspension of lecithin and cholesterol dropwise while stirring, and continue stirring until the ethanol is completely volatilized. Sonicate the liposome suspension in a water bath for 15 min to obtain a milky yellow translucent liposome suspension, dialyze for 4-5 hours in the dark, filter with a filter membrane, and the encapsulation rate is 80.80%, then place it in a brown bottle and seal it tightly , stored in the refrigerator at 4°C, and set aside.

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Abstract

The invention discloses a method for preparing a nanometer iron citrate liposome. The method comprises the following steps: 1, weighing soybean lecithin and cholesterol according to a certain mass ratio, dissolving soybean lecithin and cholesterol in anhydrous ethanol, and using a PBS buffer solution to prepare an iron citrate solution; 2, taking a certain volume of the iron citrate solution into a beaker, placing in a 40-60DEG C water bath, adding the above obtained soybean lecithin and cholesterol suspension in a dropwise manner while stirring, continuously stirring after the dropwise addition until ethanol completely volatilizes, carrying out water-bath ultrasonic treatment on the above obtained liposome suspension for 5-30min to obtain a creamy yellow semitransparent liposome suspension, carrying out photophobic dialysis for 4-5h, filtering by a filter membrane, placing in a brown bottle, and preserving at 4DEG C. The nanometer iron citrate liposome obtained in the invention has the advantages of structured and uniform particle size, good stability, high entrapment rate, and long-acting slow releasing effect, is used for the iron supplementation of the brains of rats through intranasal administration, and has an obviously better effect than iron citrate unentrapped by the liposome.

Description

technical field [0001] The invention relates to a method for preparing nano iron citrate liposome, which belongs to the technical field of biomedicine. Background technique [0002] Iron is one of the essential trace elements for the human body. Iron deficiency in the human body can lead to anemia. According to the statistics of the World Health Organization, the number of iron-deficiency anemia patients in the world has reached 1 billion. Iron deficiency in infants and young children can lead to developmental disorders of the nervous system, cognitive and behavioral disorders; iron deficiency is also closely related to children's ADHD, inattention, low IQ, and febrile convulsions. Studies have shown that effective iron supplementation in children with iron deficiency can correct anemia and relieve symptoms. At present, commonly used iron supplements are mainly small molecule organic acid iron salts, such as ferrous gluconate, ferrous citrate, sodium ferric ethylenediamine ...

Claims

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Application Information

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IPC IPC(8): A61K31/295A61K9/127A61K47/24A61K47/28A61P7/06
Inventor 耿丽娜李亚永于鹏郑红常彦忠
Owner HEBEI NORMAL UNIV
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