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Method for extracting active substances for fertilizers from algae

A technology of active substances and algae, applied in the field of biotechnology, can solve the problems of easy precipitation of alginic acid, complicated technology, and large damage

Inactive Publication Date: 2014-12-03
徐波勇 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The process of chemical extraction method is simple, mainly heating with strong acid and strong alkali to break the cell wall of seaweed. Nowadays, domestic seaweed fertilizer production enterprises mostly use this method, but when extracting active substances by this method, strong acid or strong alkali will not affect polysaccharides, chelate, etc. Synthetic elements are greatly damaged, and the extracted alginic acid is prone to precipitation, which affects the effect of fertilizer use
Physical extraction methods are mainly ultrasonic extraction and supercritical extraction. This method requires high equipment and complicated technology. Currently, it is only suitable for laboratory research and is not suitable for enterprise research and promotion.

Method used

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  • Method for extracting active substances for fertilizers from algae

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Comparison scheme
Effect test

Embodiment 1

[0011] Step 1: After mixing the dry seaweed powder and water with a pH close to neutral at a ratio of 1:20 to 1:10, enter the superfine pulverizer to shear and homogenize.

[0012] The second step: the homogenized sample is refluxed under reduced pressure, and the temperature is controlled at 50°C-70°C for water extraction; after water extraction, the extract is concentrated to below the original volume of 2096. The initial extract is placed in 40 times of 11% alkaline solution, extracted at 2-6°C for 10 hours, adjusted to a pH value of about 7.0 with acetic acid, centrifuged after the extraction is completed, and the filtrate is collected. The filtrate contains seaweed polysaccharides with small molecular weight. The active substance can be obtained by desalination under reduced pressure. The filter cake enters the next step of processing.

[0013] Step 3: After the filter cake is homogenized in water at a ratio of 1:10, it is put into a biochemical reaction tank for enzymol...

Embodiment 2

[0016] Step 1: After mixing the dry seaweed powder and water with a pH close to neutral at a ratio of 1:20 to 1:10, enter the superfine pulverizer to shear and homogenize.

[0017] The second step: the homogenized sample is concentrated under reduced pressure, the volume is reduced to 10% of the original volume, 9 times the volume of ethanol is added, refluxed for 3 hours, filtered, the filtrate is collected, the filtrate is concentrated to a paste under pressure, and the primary extraction of green algae is obtained. things. The initial extract is placed in 40 times of 11% alkaline solution, extracted at 2-6°C for 10 hours, adjusted to a pH value of about 7.0 with acetic acid, centrifuged after the extraction is completed, and the filtrate is collected. The filtrate contains seaweed polysaccharides with small molecular weight. The active substance can be obtained by desalination under reduced pressure. The filter cake enters the next step of processing.

[0018] Step 3: Aft...

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Abstract

The invention relates to a method for extracting active substances for fertilizers from algae, and belongs to the biotechnological field. Algal polysaccharides, alginic acid, chelated trace elements and other effective components are extracted from dry alga powder through homogenizing, alcohol extraction, water extraction, cold alkali liquid extraction, cellulose-protein compound enzyme hydrolysis and ultrafilter membrane separation technologies, and lay a foundation for the production of algal biological fertilizers. The method is mainly characterized by the application of the compound enzyme and the determination of enzymatic hydrolysis conditions. Cellulase and protease are selected by the inventor to carry out a biochemical reaction in a stage manner. Polysaccharides, alginic acid and other substances in alga cells are precipitated in the invention, and the activities of the above substances are maintained. Enzymatic hydrolyzed extract products undergo ultrafilter membrane separation to remove substances with the molecular weight of above 8,000,000 in order to make nutrition components concentrated and the active substances easily absorbed by plants. The extraction method using the compound enzyme hydrolysis extraction and ultrafilter membrane separation technologies to optimize the algal active components is initiated by the inventor.

Description

Technical field: [0001] The invention discloses a method for extracting fertilizer active substances from seaweed, which belongs to the field of biotechnology. In order to extract active ingredients from seaweed to produce seaweed fertilizer. We use cellulase and protease, adopt biochemical reaction extraction technology, decompose seaweed cell wall, extract seaweed polysaccharide, alginic acid, chelated trace elements and other active ingredients, and separate the molecular weight of more than 8 million through micron-scale membrane separation technology Ultra-high-molecular substances make fertilizer nutrients more easily absorbed by plants. The complex enzyme technology can ensure mild biochemical reaction conditions, avoid the decomposition of polysaccharides and chelated elements, and ensure the activity of active substances. Background technique: [0002] There are more than 60 kinds of nutrients available in seaweed, among which seaweed polysaccharides, alginic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/00
Inventor 徐波勇方文胜王磊磊安山舒新军范飞飞张盼盼张春义
Owner 徐波勇
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