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Synthesis method of nanoparticle surface modifier

A surface modifier and nanoparticle technology, applied in the field of nanomaterials, can solve problems such as lack of assembly types

Active Publication Date: 2014-12-03
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is a lack of robust, general, and controllable array assembly types for sugars to achieve arrays of sugar molecules.

Method used

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  • Synthesis method of nanoparticle surface modifier
  • Synthesis method of nanoparticle surface modifier
  • Synthesis method of nanoparticle surface modifier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. At room temperature, 2.5g, at room temperature, 2.5g, 0.0139mol (1eq) powdered mannose (MW180.16) dissolved in 20mL NaSO 4 Add 10 mL, 0.083 mol (1.5 eq) of acetic anhydride to the dried pyridine, stir for 16 h and then 70°C rotary steam, extract with ethyl acetate, 2×100 mL saturated NaHCO 3 Solution, washed with 2×100mL saturated saline, then rotary evaporated at 40°C, and dried in vacuum; fully acetylated mannose was obtained.

[0022] 2.6.4mL BF 3 .Et 2 O (7.2g, 5eq, 51.2mmol) was added dropwise to 0°C, and the CH 2 Cl 2 solution (20mL, dry), and then the mixture can be reacted at room temperature for 24h (with constant stirring). with 30mL CH 2 Cl 2 Extraction, 20mL NaHCO 3 Washed twice, Na 2 SO 4 dry. The crude product can be separated with ethyl acetate:petroleum ether=1:1 to obtain a purified product.

[0023] 3. (0.2g, 0.37mmol) the product of step 2 was dissolved in 5mL methanol in a round bottom flask, added (0.03g, 0.55mmol) NaOMe stirred at roo...

Embodiment 2

[0027] 1. At room temperature, 2.5g, at room temperature, 2.5g, 0.0139mol (1eq) powdered mannose (MW180.16) dissolved in 20mL NaSO 4 Add 10 mL, 0.083 mol (1.5 eq) of acetic anhydride to the dried pyridine, stir for 16 h and then 70°C rotary steam, extract with ethyl acetate, 2×100 mL saturated NaHCO 3 The solution was washed with 2×100 mL saturated saline, then rotary evaporated at 40° C., and dried in vacuum to obtain fully acetylated mannose.

[0028] 2.6.4mL BF 3.Et 2 O (7.2g, 5eq, 51.2mmol) was added dropwise to 0°C, and the CH 2 Cl 2 solution (20mL, dry), and then the mixture can be reacted at room temperature for 24h (with constant stirring). with 30mL CH 2 Cl 2 Extraction, 20mL NaHCO 3 Washed twice, Na 2 SO 4 dry. The crude product can be separated with ethyl acetate:petroleum ether=1:1 to obtain a purified product.

[0029] 3. (0.2g, 0.37mmol) the product of step 2 was dissolved in 5mL methanol in a round bottom flask, added (0.03g, 0.55mmol) NaOMe stirred a...

Embodiment 3

[0033] 1. At room temperature, 2.5g, at room temperature, 2.5g, 0.0139mol (1eq) powdered mannose (MW180.16) dissolved in 20mL NaSO 4 Add 10 mL, 0.083 mol (1.5 eq) of acetic anhydride to the dried pyridine, stir for 16 h and then 70°C rotary steam, extract with ethyl acetate, 2×100 mL saturated NaHCO 3 Solution, washed with 2×100mL saturated saline, then rotary evaporated at 40°C, and dried in vacuum; fully acetylated mannose was obtained.

[0034] 2.6.4mL BF 3 .Et 2 O (7.2g, 5eq, 51.2mmol) was added dropwise to 0°C, and the CH 2 Cl 2 solution (20mL, dry), and then the mixture can be reacted at room temperature for 24h (with constant stirring). with 30mL CH 2 Cl 2 Extraction, 20mL NaHCO 3 Washed twice, Na 2 SO 4 dry. The crude product can be separated with ethyl acetate:petroleum ether=1:1 to obtain a purified product.

[0035] 3. (0.2g, 0.37mmol) the product of step 2 was dissolved in 5mL methanol in a round bottom flask, added (0.03g, 0.55mmol) NaOMe stirred at roo...

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Abstract

The invention provides a synthesis method of a nanoparticle surface modifier. A chemical synthesis method is utilized to combine water-soluble dithiodiols with mannitose to obtain dithiodiol mannitose derivatives. The products can be used for carrying out functionalization modification on Au, Ag, Cd and other metals and semiconductor material surfaces under the covalent combination action of the sulfhydryl group, and the bare mannitose structure on the other end can be specifically combined with canavaline (ConA). The mannitose derivative modified / silver nanoparticle process and the specific recognition process of ConA and mannitose can be characterized by obvious changes (generally red shift) of the SPR image color of the mono nanoparticles in real time under a dark field microscope (DFM). The saccharide-nanoparticles obtained by surface modification of the functional molecule are low in toxicity and stable in the solution, and have the advantages of favorable biocompatibility, favorable stability and efficient targeted recognition capability for trace canavaline.

Description

technical field [0001] The invention specifically relates to a method for synthesizing a nanoparticle surface modifier, which belongs to the field of nanomaterials. Background technique [0002] Protein-sugar reactions underlie many aspects of cell recognition including cell adhesion, transmission and immune response. These specific reactions occur through glycoproteins, glycolipids, and cell surface polysaccharides and lectins, proteins with sugar binding sites. Plant lectins generally have binding vacancies that are weakly exposed to solvent. Therefore, the reaction between sugar and protein is generally relatively weak. In order to increase the response strength and specificity of the reaction, many proteins capable of binding sugars are oligomers composed of multiple similar or identical monomers, each of which can bind a sugar molecule. For phytohemagglutinins participating in multivalent bond binding or forming multiple simultaneous binding reactions, an obvious bin...

Claims

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Application Information

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IPC IPC(8): C07H15/04C07H1/00G01N33/00G01N33/68
Inventor 张磊范曲立王斌胡艳玲卢晓梅黄维
Owner NANJING UNIV OF POSTS & TELECOMM
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