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High tone monascus red pigment preparation method

A technology of monascus red pigment and monascus pigment, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as hidden safety hazards of monascus orange pigment, and achieve improved color value and good quality. Application prospect, low cost effect

Inactive Publication Date: 2014-12-10
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing fermentation technology, no matter it is solid fermentation or liquid fermentation, the red yeast pigment obtained is basically a mixture of monascus red pigment, monascus orange pigment and monascus yellow pigment. Due to the potential safety hazard of monascus orange pigment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment relates to a micellar catalytic method for converting monascus orange pigment into monascus red pigment, the method comprising the following steps:

[0038] Step 1, Monascus was cultivated in seed medium water (100ml, peptone 2g, yeast powder 2g, glucose 2g) for 32 hours to obtain seed liquid, and described Monascus was provided by China Industrial Microorganism Strain Collection Center (Monascus anka, CICC 5013);

[0039] Step 2, inoculate the seed liquid into a fermentation culture liquid with an initial pH value of 4.5 and corn flour as a nitrogen source, carry out fermentation culture in a shaker at a temperature of 30°C and 200 rpm for 7 days, and centrifuge to obtain red Wet cells of Aspergillus; take wet cells of Monascus for pretreatment, release intracellular products, detect the pH of the fermentation broth, and the color value of the red yeast pigment inside and outside the cells; wherein, the pretreatment is specifically: take 0.5g of wet cel...

Embodiment 2

[0044] This embodiment relates to a micelle-catalyzed method for converting monascus orange pigment into monascus red pigment, the method comprising the following steps:

[0045] Step 1, Monascus was cultivated in seed medium water (100ml, peptone 2g, yeast powder 2g, glucose 2g) for 32 hours to obtain seed liquid, and described Monascus was provided by China Industrial Microorganism Strain Collection Center (Monascus anka, CICC 5013);

[0046] Step 2, inoculate the seed liquid into a fermentation culture liquid with an initial pH value of 4.5 and corn flour as a nitrogen source, carry out fermentation culture in a shaker at a temperature of 30°C and 200 rpm for 7 days, and centrifuge to obtain red Wet cells of Aspergillus; take wet cells of Monascus for pretreatment, release intracellular products, detect the pH of the fermentation broth, and the color value of the red yeast pigment inside and outside the cells; wherein, the pretreatment is specifically: take 0.5g of wet cell...

Embodiment 3

[0051] This embodiment relates to a micelle-catalyzed method for converting monascus orange pigment into monascus red pigment, the method comprising the following steps:

[0052] Step 1, Monascus was cultivated in seed medium water (100ml, peptone 2g, yeast powder 2g, glucose 2g) for 32 hours to obtain seed liquid, and described Monascus was provided by China Industrial Microorganism Strain Collection Center (Monascus anka, CICC 5013);

[0053] Step 2, inoculate the seed liquid into a fermentation culture liquid with an initial pH value of 4.5 and corn flour as a nitrogen source, carry out fermentation culture in a shaker at a temperature of 30°C and 200 rpm for 7 days, and centrifuge to obtain red Wet cells of Aspergillus; take wet cells of Monascus for pretreatment, release intracellular products, detect the pH of the fermentation broth, and the color value of the red yeast pigment inside and outside the cells; wherein, the pretreatment is specifically: take 0.5g of wet cell...

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Abstract

The invention provides a high tone monascus red pigment preparation method which comprises the following steps: 1, culturing monascus in a seed culture medium to obtain a seed liquid; 2, inoculating the seed liquid into a fermentation culture liquid for fermentation culture, regulating the pH value of the fermentation culture liquid, centrifuging to obtain monascus wet cells, and extracting intracellular monascus pigment; 3, putting the extracted intracellular monascus pigment into a surfactant micellar solution, and adding sodium glutamate to realize micellar catalysis for converting monascus orange pigment into monascus red pigment; or inoculating the seed liquid obtained by the step 1 into a fermentation culture medium containing a nonionic surfactant, adding the sodium glutamate, regulating the pH value of the fermentation culture medium for culturing to obtain the monascus red pigment. According to the method, the surfactant and the sodium glutamate are added into the fermentation culture medium for preparation of the high tone and high color value monascus red pigment with low content of the monascus orange pigment.

Description

technical field [0001] The invention relates to a fermentation method in the technical field of bioengineering, in particular to a method of using a surfactant to secrete intracellular products and further realizing micelle catalysis outside the cells to prepare a high-color (relatively single component) monascus pigment method. Background technique [0002] Red yeast rice produced by solid-state fermentation of Monascus sp is a traditional food coloring additive in China and a traditional Chinese medicine, which has been used in my country for more than a thousand years. Today, modern microbial liquid submerged fermentation technology is used to produce monascus red pigment, which is widely used as a food color additive in East Asian countries, such as China, Japan, South Korea, Taiwan, Thailand, Vietnam, etc. Generally, the monascus pigments produced by the fermentation of Monascus include two components of monascus yellow pigment, two components of monascus orange pigmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C09B61/00C12R1/645
Inventor 王志龙熊旭
Owner SHANGHAI JIAO TONG UNIV
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