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Method for reducing content of volatile basic nitrogen in active peptides of euphausia superba

A technology of volatile base nitrogen and Antarctic krill, which is applied in the field of food and medical supplies processing, can solve the problems of quality decline and discoloration of active peptide powder, and achieve the effects of simple operation, high efficiency and low equipment investment.

Active Publication Date: 2014-12-10
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, if the water-soluble Antarctic krill active peptide is exposed to high-temperature steam and aerobic environment for a long time, it will cause changes such as discoloration, resulting in a decline in the quality of the active peptide powder

Method used

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  • Method for reducing content of volatile basic nitrogen in active peptides of euphausia superba

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Experimental program
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Effect test

Embodiment 1

[0040] 1) Take 5kg of Antarctic krill powder, add 50L of distilled water to the shrimp powder to obtain a mixed solution, add 2M HCl or 2M NaOH to the mixed solution to adjust the pH value of the mixed solution to 8.0, raise the temperature to 50°C, and add alkali to the mixed solution 80g of active protease, stirred in the reaction kettle for 10h, heated to 100°C to terminate the enzyme reaction, centrifuged to take the supernatant, after the supernatant was defluorinated, the defluorinated krill active peptide aqueous solution A was obtained;

[0041] 2) Make the solid content of solution A reach 15% by vacuum concentration, the vacuum degree is 0.08MPa, the temperature is 60°C, and solution B is obtained;

[0042] 4) Take 10L of solution B, and add 15g of calcium hydrogen phosphate to it to obtain mixed solution C;

[0043] 5) Heating the mixed solution C under vacuum conditions for 3 hours, the vacuum degree is 0.08MPa, the temperature is 60°C, and centrifuged after coolin...

Embodiment 2

[0048] 1) Take 10 kg of Antarctic krill powder, add 100 L of 95% ethanol to the shrimp powder, stir in the reaction kettle for 6 hours, centrifuge to take the precipitate, and vacuum-dry to obtain defatted krill powder;

[0049] 2) Take 5kg of defatted shrimp powder, add 60L of distilled water to the defatted shrimp powder to obtain a mixed solution, add 2M HCl or 2M NaOH to the mixed solution to adjust the pH value of the mixed solution to 7.5, raise the temperature to 45°C, and add Chinese 100g of active protease, stirred in the reaction kettle for 8 hours, heated to 100°C to terminate the enzyme reaction, centrifuged to take the supernatant, after the supernatant was defluorinated, the defluorinated krill active peptide aqueous solution A was obtained;

[0050] 4) Make the solid content of solution A reach 20% by vacuum concentration, the vacuum degree is 0.07MPa, and the temperature is 50°C to obtain solution B;

[0051] 5) Take 10L of solution B, and add 30g of potassium ...

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Abstract

The invention aims at providing a method for reducing the content of volatile basic nitrogen in active peptides of euphausia superba. The method comprises the following steps: enzymatically hydrolyzing euphausia superba powder, and defluorinating to obtain an active peptide aqueous solution; adjusting the solid content of the active peptide aqueous solution of the euphausia superba by diluting or concentrating, and adding a swelling agent into the active peptide aqueous solution of the euphausia superba; heating the active peptide aqueous solution of the euphausia superba in vacuum for a period of time, cooling, and centrifuging to obtain supernatant; desalting the supernatant, and spray-drying to obtain an euphausia superba active peptide powder product with low volatile basic nitrogen content. The method is simple to perform, low in equipment investment, free of environmental pollution, high in efficiency and suitable for industrialized operation; the obtained euphausia superba active peptide powder with low volatile basic nitrogen content has the characteristics of no harmful material residues and high product safety.

Description

technical field [0001] The invention belongs to the technical field of food and medical product processing, and in particular relates to a method for reducing the content of volatile base nitrogen in water-soluble Antarctic krill active peptide. Background technique [0002] Volatile basic nitrogen refers to alkaline nitrogen-containing substances such as ammonia and amines produced by the decomposition of protein in food during the process of animal food spoilage under the action of bacteria and enzymes. Volatile basic nitrogen is a class of volatile toxic substances. The content of volatile basic nitrogen is an important indicator of the freshness of processed aquatic products, and it has a clear correlation with the freshness of raw materials and the processing process. [0003] Antarctic krill is a class of marine invertebrates distributed in Antarctic waters. According to statistics, the reserves of Antarctic krill are 650-100 million tons, which is a huge treasure ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 李兆杰王彦超薛长湖常耀光薛勇王玉明何晨
Owner OCEAN UNIV OF CHINA
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