Method for evaluating biosecurity of 35 nm nano silver on the basis of intestinal epithelial cells

Abstract

The invention provides a method for evaluating the biosecurity of 35 nm nano silver on the basis of intestinal epithelial cells. According to the method, cell lines of human colon carcinoma epithelial cells Caco-2 are selected as an object of study, nano zinc oxide (26.21 nm) with higher cytotoxicity is taken as a positive control, normal cells without the nano material are taken as blank control, and the cytotoxicity of the nano material is preliminarily explored by an AO / EB double staining method and a WST-1 method. The oxidative damage effect and the possible mechanism of nano silver on cells are studied by an oxidative stress method through detecting the influence of emission of ROS, SOD and GSH on the cells Caco-2. The experimental result shows that a low dose (smaller than 50 mu g / ml) of 35.48 nm silver particles cannot influence the activity of the intestinal epithelial cells, and causes no oxidative stress damage; and 75 mu g / ml of 35.48 nm silver particles and 100 mu g / ml of 35.48 nm silver particles have a dose-effect relation and a time-effect relation with the cytotoxicity of intestinal epithelial cells (Caco-2), and cause minor oxidative stress damage on the cells.

Description

technical field [0001] The invention belongs to the field of food safety, in particular to a method for evaluating the biological safety of 35.48nm nano-silver based on intestinal epithelial cells. Background technique [0002] Nano-silver has stronger antibacterial properties than conventional silver, and a small amount of nano-silver can produce a strong bactericidal effect. With its excellent antibacterial properties, nano silver has been widely used in the food industry and has become one of the research hotspots in recent years. However, nano silver particles used in the food field can migrate, invade the human body through the digestive tract, and may accumulate in the deep. Therefore, the food safety issue of nano-silver deserves attention. [0003] In recent years, commercialized nanomaterials are mainly concentrated on materials such as silver, carbon, zinc oxide, silicon, titanium dioxide, and gold. Most of the nanocommodities involve metallic silver, accounting ...

AI Technical Summary

Problems solved by technology

At present, most of the toxicological researches on nanosilver are in the field of medicine, and the research objects are medical nanosilver. The research methods are mostly animal experiments and in vitro cell culture. There are few studies on the safety evaluation of food nanosilver.

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