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LED-based Multi-window and multi-path detection method of digital polymerase chain reaction PCR

A technology of chain reaction and detection method, applied in the field of microfluidics, to achieve the effect of easy automation, simple control scheme and low overall cost

Inactive Publication Date: 2014-12-10
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no method of opening a series of windows along the sample channel in the prior art, and each window adopts the method of LED light detection

Method used

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  • LED-based Multi-window and multi-path detection method of digital polymerase chain reaction PCR
  • LED-based Multi-window and multi-path detection method of digital polymerase chain reaction PCR

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Embodiment 1

[0025] Such as figure 1 As shown, the present invention is a schematic flow diagram of digital PCR-based multi-point multiplex detection, which is also a schematic flow diagram of an implementation of the present invention.

[0026] A plurality of transparent windows (1) are opened on the capillary (2) at the end of the digital PCR, and liquid droplets (3) can be seen in each window; each detection window is detected by a single light source; the detection light source is a single-color LED. Among them, multiple detection light sources are located above the channel; the wall at the corresponding position of the channel is optically transparent, and the window size is the characteristic size of the droplet; below the channel is a photodiode (Photo-Diode), and the electrical signal generated is imported into the subsequent processing system. All components are integrated on a flat panel and finally sealed in a case.

[0027] The specific embodiment of the present invention is t...

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Abstract

The invention provides an LED-based multi-window and multi-path detection method of digital polymerase chain reaction PCR. The method comprises the following steps: the LEDs are adopted as detection light sources, transparent windows are arranged at the tail end of a capillary pipeline in a segmentation manner after the digital PCR finishes polymerase chain reaction; each LED with one type of wavelength is responsible for detecting one window, a type of signal possibly sent by sample drops is detected. Since the drops of the digital PCR are ordered, multiple signals in each sample drop can be detected and accurately counted. Compared with the method of detecting the same drop by adopting multiple bundles of laser, the method is simpler and more feasible, has the characteristics of simple structure and low cost, and also can be used as an inserting piece to be combined with the existing detection equipment so as to increase the working speed of daily blood detection in a hospital.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, in particular to an LED-based digital polymerase chain reaction (PCR) multi-window and multi-channel detection method. Background technique [0002] Polymerase chain reaction (PCR) uses DNA denaturation at a high temperature of 95°C in vitro to become a single strand. At low temperature (usually around 60°C), primers and single strands are combined according to the principle of complementary base pairing, and then the temperature is adjusted. To the optimum reaction temperature of DNA polymerase (about 72°C), DNA polymerase synthesizes complementary chain along the direction from phosphoric acid to five-carbon sugar (5'-3'). The PCR instrument is actually a temperature control device, which can effectively control the denaturation temperature, renaturation temperature, and extension temperature of the sample; in the digital PCR instrument, the sample exists in the form of droplets, and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/05G01N21/01
Inventor 何立群罗昭锋张海峰
Owner UNIV OF SCI & TECH OF CHINA
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