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Stable detection kit of total bile acid

A technology for detecting kits and total bile acids, applied in the field of medical immunology in vitro diagnosis, can solve the problems of difficult and long-term storage of NADH, poor reagent stability, short storage time, etc., and achieves improved accuracy, high sensitivity, and reduced dosage. Effect

Active Publication Date: 2014-12-10
上海睿康生物科技有限公司
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Problems solved by technology

[0010] However, there are still problems in the above-mentioned assay method. Because NADH is difficult to preserve for a long time in the solution, the stability of the reagent is poor and the preservation time is short.

Method used

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  • Stable detection kit of total bile acid
  • Stable detection kit of total bile acid
  • Stable detection kit of total bile acid

Examples

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Embodiment 1～3

[0036] The present examples 1-3 relate to a stable total bile acid detection kit, the kit includes reagent R1 and reagent R2, and the components and concentrations of the reagents R1 and R2 are shown in Table 1:

[0037] Table 1

[0038]

[0039] The TBA detection kits described in Examples 1 to 3 are applicable to various types of automatic biochemical analyzers. Taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 2. Analysis method: Two-point endpoint method, that is, reagent R1; the amount of R2 is 270ul and 90ul respectively, and the sample volume is 4ul; 270ul reagent R1 is added to 4ul sample, and 90ulR2 is added after 5 minutes at 37°C, and the reading point is started after a delay of 60 seconds. The reading time is about 180 seconds; the detection wavelengths are respectively main wavelength 405nm and sub-wavelength 600nm.

[0040] Adopt this reagent and above-mentioned assay method, adopt the curve of the TBA standard...

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Abstract

The invention provides a stable detection kit of total bile acid. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components: a buffering solution, beta-thionicotinamide adenine dinucleotide, a surface active agent, a stabilizing agent and a preservative; the reagent R2 comprises the following components: a buffering solution, reduction type coenzyme I (NADH), 3alpha-hydroxysteroid, a surface active agent, antioxidant enzyme, a stabilizing agent and a preservative. The stable detection kit provided by the invention has the advantages that since the antioxidant enzyme is added into the reagents, NADH can be stored for a long time, the influence caused by more interference components in the reaction process is eliminated, so that the NADH can be utilized efficiently, the speed of circulating reaction is increased, the detection accuracy is improved, the sensitivity is high, the linear range is wide, simultaneously, the used amount of main reaction materials can be reduced and the cost of the reagents is reduced.

Description

technical field [0001] The invention relates to the field of medical immune in vitro diagnosis, in particular to a stable total bile acid detection kit. Background technique [0002] Bile acids synthesized by normal human liver include cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA) produced in metabolism, and a small amount of lithocholic acid (LCA) and trace ursodeoxycholic acid (UDCA). , collectively referred to as total bile acids (TBA). [0003] Human serum total bile acids (TBA) are synthesized and catabolized by the liver to maintain the relative stability of human bile acids, and its regulation is a major function of the liver. When the liver cells have lesions or suffer from bile duct disease, it can cause bile metabolism disorder, which will significantly increase the content of bile acids entering the blood, and the increase of serum TBA is directly proportional to the degree of liver cell damage. [0004] Bile acid is the product of chole...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N33/53G01N35/00
Inventor 李伟奇李杰房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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