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Preparation method of novel escherichia coli electrochemical sensor

An Escherichia coli, electrochemical technology, applied in the field of preparation of Escherichia coli electrochemical sensors, can solve the problems of unfavorable popularization and promotion

Inactive Publication Date: 2014-12-10
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, both of the above two new methods require professional and expensive detection instruments to characterize the detection process, and highly professional personnel are required to perform experimental operations, so these methods are not conducive to popularization

Method used

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  • Preparation method of novel escherichia coli electrochemical sensor
  • Preparation method of novel escherichia coli electrochemical sensor
  • Preparation method of novel escherichia coli electrochemical sensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. The preparation of Escherichia coli electrochemical sensor comprises the following two steps:

[0021] 1.1 Carboxylation of glassy carbon electrodes: glassy carbon electrodes should be pretreated before use. The glassy carbon electrode was polished in alumina slurry with a particle size of 50nm for 5min, then ultrasonically cleaned in 0.1mol / L nitric acid, absolute ethanol and ultrapure water for 1min, and finally the electrode surface was dried with high-purity nitrogen. The cleaned glassy carbon electrode was activated by cyclic voltammetry in a 0.1mol / L sulfuric acid solution through an electrochemical workstation. After meeting the requirements, it was soaked in ultrapure water for later use. The above-mentioned treated glassy carbon electrode was immersed in 0.05mol / L sodium hydroxide solution for surface carboxylation, and cyclic voltammetry was used to scan for 1 circle, with a scanning speed of 50mV / s and a scanning range of -0.1 to 1.4V.

[0022] 1.2 Coupli...

Embodiment 2

[0026] 1. The preparation of Escherichia coli electrochemical sensor comprises the following two steps:

[0027] 1.1 Carboxylation of glassy carbon electrodes: glassy carbon electrodes should be pretreated before use. The glassy carbon electrode was polished in alumina slurry with a particle size of 50nm for 10min, then ultrasonically cleaned in 1.0mol / L nitric acid, absolute ethanol and ultrapure water for 3min, and finally the electrode surface was dried with high-purity nitrogen. The cleaned glassy carbon electrode was activated by cyclic voltammetry in a 1.0mol / L sulfuric acid solution through an electrochemical workstation. After meeting the requirements, it was soaked in ultrapure water for later use. The above-mentioned treated glassy carbon electrode was immersed in 1mol / L sodium hydroxide solution for surface carboxylation, and was scanned by cyclic voltammetry for 5 cycles at a scanning speed of 100mV / s and a scanning range of -0.5 to 1.5V.

[0028] 1.2 Coupling of ...

Embodiment 3

[0032] 1. The preparation of Escherichia coli electrochemical sensor comprises the following two steps:

[0033] 1.1 Carboxylation of glassy carbon electrodes: Glassy carbon electrodes should be pretreated before use. The glassy carbon electrode was polished in alumina slurry with a particle size of 50nm for 10min, then ultrasonically cleaned in 0.5mol / L nitric acid, absolute ethanol and ultrapure water for 6min, and finally the electrode surface was dried with high-purity nitrogen. The cleaned glassy carbon electrode was activated by cyclic voltammetry in a 0.5mol / L sulfuric acid solution through an electrochemical workstation. After meeting the requirements, it was soaked in ultrapure water for later use. The above-mentioned treated glassy carbon electrode was immersed in 1mol / L sodium hydroxide solution for surface carboxylation, and cyclic voltammetry was used to scan 10 cycles at a scanning speed of 150mV / s and a scanning range of -1 to 2V.

[0034] 1.2 Coupling of Esche...

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Abstract

The invention relates to a preparation method of a novel escherichia coli electrochemical sensor. According to the technical scheme, the surface of a glassy carbon electrode is carboxylated, and escherichia coli is linked to the surface of the glassy carbon electrode by virtue of a biologic coupling technique so as to prepare the escherichia coli electrochemical sensor; the glassy carbon electrode is taken as a substrate, the surface of the glassy carbon electrode is subjected to sodium hydroxide etching to be carboxylated, and then the escherichia coli is coupled to the surface of the glassy carbon electrode, wherein a coupling agent is 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide easer (NHS). The preparation method is characterized in that the escherichia coli electrochemical sensor with high sensitivity is obtained. The escherichia coli electrochemical sensor is high in stability and detection sensitivity, good in accuracy, and the detection efficiency is obviously superior to that of a conventional detection method. The preparation method comprises the following steps: (1) preparing an escherichia coli section chemical sensor; and (2) detecting electrochemical signals of the escherichia coli section chemical sensor. The novel escherichia coli electrochemical sensor is simple and convenient in preparation method, high in detection sensitivity and accuracy and high detection speed and is convenient for practical application.

Description

technical field [0001] The invention relates to Escherichia coli detection technology, in particular to preparation of an Escherichia coli electrochemical sensor, and rapid detection of Escherichia coli based on changes in electrical signals. The sensor organically combines electrochemical technology with biotechnology, and detects Escherichia coli in a liquid environment through peak signals of cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Background technique [0002] Escherichia coli is a resident bacterium in the intestines of humans and animals. It is often excreted together with human and animal feces, widely spread in the natural environment, and pollutes water resources. Most Escherichia coli are not pathogenic, but some of them can produce enterotoxin, which can cause human gastroenteritis and other diseases. In particular, Escherichia coli O157 can cause diseases such as diarrhea, hemorrhagic colitis, and hemolytic uremia. When it invades othe...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/26
Inventor 许世超郑晓玥张晨赵丽明张雪平李润兰
Owner TIANJIN POLYTECHNIC UNIV
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