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A kind of biodegradable cell microcarrier and preparation method thereof

A technology of microcarriers and materials, applied in the field of biomedicine, can solve the problems of affecting application, adverse cell adhesion due to hydrophobicity, etc., and achieve the effects of increasing the amount of adhesion, improving hydrophilicity and simple preparation method.

Active Publication Date: 2017-04-12
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrophobicity of PLA or PLGA is not conducive to cell adhesion, which seriously affects its application in the preparation of microcarriers.

Method used

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  • A kind of biodegradable cell microcarrier and preparation method thereof
  • A kind of biodegradable cell microcarrier and preparation method thereof
  • A kind of biodegradable cell microcarrier and preparation method thereof

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preparation example Construction

[0044] The present invention also provides a method for preparing the modified microcarrier, including the following steps:

[0045] A) Provide microcarriers, the material of which is polylactic acid and / or polylactic acid-glycolic acid copolymer;

[0046] B) Mixing the microcarrier in the step A) with the dopamine solution to perform a modification reaction to obtain a modified microcarrier.

[0047] In the present invention, the types and sources of the microcarriers are consistent with the types and sources of the microcarriers described in the above technical solution, and will not be repeated here.

[0048] In the present invention, the microcarrier is mixed with the dopamine solution to perform a modification reaction to obtain a modified microcarrier. In the present invention, preferably, dopamine and Tris / HCL buffer are mixed to obtain a dopamine solution, and the dopamine solution is mixed with a microcarrier to perform a modification reaction to obtain a modified microcarrie...

Embodiment 1

[0066] Dissolve 0.05g of HA and 0.8g of PLGA with a number average molecular weight of 50000 in 15mL of chloroform to obtain a mixed solution; add 15mL of the mixed solution dropwise to 100mL of 1% PVA aqueous solution, and stir at 400rpm for 28 hours to obtain Microcarriers. The obtained microcarriers were washed three times with one water centrifugation, and then vacuum dried for 48 hours to obtain dry microcarriers.

[0067] Dopamine was dissolved in a Tris / HCL buffer with a molar concentration of 10 mM and a pH of 8.5 to obtain a dopamine solution with a concentration of 4 mg / ml. The microcarrier was immersed in the dopamine solution and stirred for 6h to obtain the modified microcarrier. The obtained modified microcarriers were washed 5 times with one water centrifugation.

[0068] IGF-1 was dissolved in a dopamine solution to obtain an active substance solution with a mass concentration of 1 ng / mL, the modified microcarrier was immersed in the active substance solution, and...

Embodiment 2

[0076] 0.06 g of PLGA with a number average molecular weight of 1.0 was dissolved in 10 mL of chloroform to obtain a mixed solution; 10 mL of the mixed solution was added dropwise to 100 mL of a 1% PVA aqueous solution, and stirred at 400 rpm for 28 hours to obtain microcarriers. The obtained microcarriers were washed three times with one water centrifugation, and then vacuum dried for 48 hours to obtain dry microcarriers.

[0077] The dopamine was dissolved in a Tris / HCL buffer with a molar concentration of 10 mM and a pH of 8.5 to obtain a dopamine solution with a concentration of 2 mg / mL. The microcarrier was immersed in the dopamine solution and stirred for 6h to obtain the modified microcarrier. The obtained modified microcarriers were washed 5 times with one water centrifugation.

[0078] IGF-1 was dissolved in a dopamine solution to obtain an active substance solution with a mass concentration of 1 ng / mL, the modified microcarrier was immersed in the active substance soluti...

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Abstract

The invention provides a modified microcarrier, which is obtained by modifying the microcarrier through a modifier; the microcarrier is polylactic acid and / or polylactic acid-glycolic acid copolymer; the modifier contains catechol substance. The present invention modifies polylactic acid (PLA) and / or polylactic acid-glycolic acid copolymer (PLGA) by substances containing catechol groups, and forms a polydopamine layer on the surface of PLA and / or PLGA, so that PLA and / or the hydrophilicity of PLGA has been significantly improved, increasing the amount of cell adhesion. The invention also provides a preparation method of the modified microcarrier, which is simple and efficient. The present invention also provides a functional microcarrier. The active substance on the functional microcarrier provided by the present invention can induce cells in a directional way to obtain functionalized cells, which can be directly applied to tissue repair.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to a modified microcarrier, a preparation method thereof, and a functional microcarrier. Background technique [0002] Microcarrier culture technology is to inoculate cells on microcarriers to achieve rapid, large-scale and high-quality expansion of cells in a dynamic three-dimensional culture environment in order to obtain a sufficient number of cells for tissue engineering and stem cell therapy. [0003] Commonly used microcarriers are divided into degradable (such as polylactic acid, gelatin, etc.) and non-degradable microcarriers (such as plastic, glass, etc.). Although microcarriers made of non-degradable materials (such as glass, polystyrene, etc.) can be used for large-scale cell expansion, their poor biocompatibility and inability to directly use them in the body restrict their wider application. Compared with non-degradable microcarriers, degradable microcarriers can not onl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08A61L27/18A61L27/54
Inventor 章培标高田林陈学思王宗良王宇黄晶张宁
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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