Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

The Attenuated Mutation Strain of Nocardia Amberatus and Its Application

A technology of Nocardia amberjack and Nocardia, which is applied in bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the loss of feasibility of inactivated vaccine development routes and the development process of fish Nocardia vaccines. It is not ideal and cannot provide immune protection, so as to eliminate the possibility of spreading a large number of virulent pathogens, have a significant immune effect, and achieve a good control effect.

Active Publication Date: 2017-04-26
SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Worldwide, however, the development of vaccines against Nocardia spp. for fish has been suboptimal because, unlike other fish pathogens, it has been found that multiple inactivated vaccines against Nocardia spp. effect, which makes the development route of inactivated vaccines unfeasible

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The Attenuated Mutation Strain of Nocardia Amberatus and Its Application
  • The Attenuated Mutation Strain of Nocardia Amberatus and Its Application
  • The Attenuated Mutation Strain of Nocardia Amberatus and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 attenuated live vaccine

[0022] (1) Mutation, screening and identification of the mutagenized attenuated strain of Nocardia japonica

[0023] 1. Preparation of Nocardia wild strain ZJ0503 bacterial suspension from yellowtail

[0024]In March 2005, our laboratory isolated a wild yellowtail Nocardia strain ZJ0503 (Huang Yucong et al. , Isolation and Identification of the Pathogen of Sarcoidosis in Pompano ovata, Journal of Guangdong Ocean University, 2008,28(4):49-53). The amberjack Nocardia ZJ0503 strain stored in a -80°C refrigerator was streaked on BHI solid medium, cultured upside down at a constant temperature of 28°C for 7 days, a single colony was picked and placed in a sterile phosphate buffer, and mixed to prepare bacterial suspension.

[0025] 2. UV mutagenesis

[0026] Pipette 0.1 ml of the ZJ0503 bacterial suspension and spread it on the modified solid medium plate of Nocardia yellowtail containing 0.3% to 0.5% lithium chlo...

Embodiment 2

[0053] Example 2 Detection of the pathogenicity of the mutagenized attenuated strain NSX1 of the yellowtail Nocardia

[0054] Under laboratory conditions, zebrafish was used as a model animal to detect the toxicity of the attenuated strain NSX1 of Nocardia amberii obtained through mutagenesis screening. The zebrafish (average body length 2.5-3cm, body weight about 0.2g) used in the experiment was first placed in a clean water tank to adapt to breeding for 1 week to eliminate abnormal individuals, and the mortality rate should be lower than 2%. Before the infection test, the test fish were divided into random groups, and each group was tested in parallel with 2 water tanks, and 50 fish were cultured in each tank (20 L). The test water tank uses tap water that has been oxygenated for more than 24 hours to replace 1 / 2 volume of aquaculture water every day, and the water temperature is 28±2°C. In the infection test, each group of test fish was treated with a certain gradient dose...

Embodiment 3

[0058] Example 3 Evaluation of the immune protection rate of Nocardiella mutagenic attenuated strain NSX1 from yellowtail

[0059] Zebrafish were used as experimental animals to carry out the immune protection test. The experimental zebrafish were randomly divided into 3 groups, each group had 3 parallel water tanks, 16 fish / box. The attenuated strain NSX1 was used as a live vaccine to immunize zebrafish by intraperitoneal injection, and the immunization dose was 10 7 CFU / tail. The control group was injected with sterile phosphate buffer. After 14 days of immunization, the zebrafish in each group were artificially infected and challenged with the wild strain ZJ0503 of Nocardia japonica, and the challenge dose was 10 6 CFU / tail. Observe and count the death numbers of the control group and the immune group within 14 days, and calculate the immune protection rate of each group (see Table 2).

[0060] Among them, the immune protection rate is calculated according to the follow...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a nocardia seriolae induced low virulent strain and an application thereof, and belongs to the technical field of bacterial strain induced mutation. Particularly, the nocardia seriolae induced low virulent strain, named NSX1 (Nocardia seriolae NSX1), of which the culture preservation number is CGMCCNO.9437. An immune compound comprises the nocardia seriolae induced low virulent strain used as an immune an immunogenicity component, wherein the concentration of the nocardia seriolae induced low virulent strain is 10<3>-10<9>CFU / ml; the nocardia seriolae induced low virulent strain is used for preparing drugs for resisting fish nocardiosis and has significant low toxicity effect; meanwhile, the fish can be effectively protected from the invasion of nocardia seriolae caused by nocardia seriolae pathogenic strains or virulence strains; therefore, the nocardia seriolae induced low virulent strain has significant immune effect and good nocardia seriolae disease control effect.

Description

technical field [0001] The invention relates to a mutagenesis of fish Nocardia and its application, in particular to a mutagenic attenuated strain of Nocardia amberjack and its application, and belongs to the technical field of strain mutagenesis. Background technique [0002] Nocardiosis in fish is one of the common diseases in pond culture, floating raft cage culture and deep water cage culture mode. It is a chronic systemic granulomatous disease. Nocardia seriolae is its main pathogen. The disease can form a large number of nodules in the body and surface of the fish, and can also cause bleeding and ulcers on the surface of the fish, which seriously affects the commercial value of adult fish. Its infection rate and mortality are high, and the natural incidence rate can reach 15~ 30%, up to 50-60% in severe cases, the average mortality rate is about 20%, and the mortality rate of artificial infection can be as high as 90%-100%. In recent years, fish nocardiosis has been ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
Inventor 夏立群陈建林鲁义善简纪常蔡佳张红莲
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com