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Method for extracting general flavones from Herba Selaginellae

A technology of total flavonoids and Selaginella, applied in the field of medicine and chemical industry, can solve the problems of undisclosed total flavonoids of Selaginella, poor swelling and wall breaking effect of Selaginella tissue, and large solvent consumption, and achieve short extraction time and low solvent consumption. Effect of low concentration and low solvent consumption

Inactive Publication Date: 2014-12-31
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor effect of tissue swelling and breaking the wall of Selaginella, multiple reflux extractions are required, resulting in large solvent consumption, long extraction time, many impurities, and high industrial production costs.
At present, there is no relevant report on the surfactant-assisted microwave extraction method of the total flavonoids of Selaginella

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Get the whole plant of Selaginella selaginellae in the air and dry, pulverize through a 50 mesh sieve, weigh 100g, add 3L of 25% (V / V) ethanol aqueous solution, add 15g sodium lauryl sulfate again, stir under 60rpm / min, adjust The microwave power is 400w / L, irradiate for 4 minutes, cool and control the temperature at 65-80°C, and repeat the irradiation 15 times. Filter the extract with a ceramic membrane, add 0.5% (V / V) hydrochloric acid solution to adjust pH 3.0, flow through the macroporous adsorption resin AB-8 for adsorption, the flow rate is 3BV / h; use 95% (V / V) ethanol Elute, concentrate under reduced pressure to remove ethanol, crystallize with 95% ethanol, filter and dry to obtain total flavonoids of Selaginella; the effluent of macroporous adsorption resin is adjusted to pH 6.0 with 0.5% NaOH, and used as the extraction solvent for the next batch of Selaginella.

Embodiment 2

[0021] Take the whole plant of Selaginella chinensis and dry it in the air, crush it through a 40-mesh sieve, weigh 100g, add 2.5L of 30% (V / V) ethanol aqueous solution, then add 20g cetyltrimethylammonium chloride, 60rpm / Under stirring for one minute, adjust the microwave power to 500w / L, irradiate for 2 minutes, cool and control the temperature at 65-80°C, and repeat the irradiation 10 times. Filter the extract with a ceramic membrane, add 0.5% (V / V) hydrochloric acid solution to adjust the pH to 3.0, flow through the macroporous adsorption resin DM130 for adsorption, the flow rate is 3BV / h; elute with 95% (V / V) ethanol , concentrated under reduced pressure to remove ethanol, crystallized with 95% ethanol, filtered and dried to obtain the total flavonoids of Selaginella; the macroporous adsorption resin effluent was adjusted to pH 6.0 with 0.5% NaOH, and used as the extraction solvent for the next batch of Selaginella.

Embodiment 3

[0023] Take the whole plant of Selaginella chinensis and dry it in the air, crush it through a 60-mesh sieve, weigh 100g, add 3.5L of 30% (V / V) ethanol aqueous solution, then add 25g of sodium dodecylbenzenesulfonate, stir at 70rpm / min Adjust the microwave power to 500w / L, irradiate for 2 minutes, cool and control the temperature at 65-80°C, and repeat the irradiation 12 times. Filter the extract with a ceramic membrane, add 0.6% (V / V) hydrochloric acid solution to adjust the pH to 3.0, flow through the macroporous adsorption resin DM130 for adsorption, the flow rate is 3BV / h; elute with 95% (V / V) ethanol , concentrated under reduced pressure to remove ethanol, crystallized with 95% ethanol, filtered and dried to obtain the total flavonoids of Selaginella; the macroporous adsorption resin effluent was adjusted to pH 6.0 with 0.5% NaOH, and used as the extraction solvent for the next batch of Selaginella.

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PUM

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Abstract

The invention discloses a method for extracting general flavones from Herba Selaginellae. A surfactant is adopted to assist the microwave extraction of general flavones from Herba Selaginellae. The method concretely comprises the following steps: drying entire Herba Selaginellae, crushing, immersing the crushed entire Herba Selaginellae in an aqueous diluted ethanol solution containing a proper concentration of the surfactant, carrying out microwave irradiation extraction many times, rapidly adding the obtained extraction liquid to a macroporous resin column for adsorption, eluting by ethanol, and drying to obtain Herba Selaginellae general flavones. The method for extracting the Herba Selaginellae general flavones by combining a microwave extraction technology with the surfactant has the advantages of reduction of the application amount of a solvent, extraction efficiency increase, low requirements on an apparatus, simple flow, and suitableness for industrial production.

Description

technical field [0001] The invention relates to a method for extracting total flavonoids from Selaginella, more specifically to a method for extracting total flavonoids from Selaginella by using surfactants to assist microwaves, and belongs to the field of medicine and chemical industry. Background technique [0002] Herba Selaginellae (Herba Selaginellae), is a plant of Lycopophyta - Lycopoda - Selaginellae - Selaginellaceae, also known as Nine Dead Resurrection Grass, which was classified as Fern in the past. Its dried whole herb has the effect of promoting blood circulation and promoting menstruation, which was recorded in the 2010 edition of "Chinese Pharmacopoeia (Part One)". In recent years, scholars at home and abroad have conducted a lot of research on the chemical components of Selaginella plants, and the active components reported are mainly flavonoids and organic acids, and chromones have also been found. Among them, flavonoids have significant pharmacological ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/11
Inventor 魏元刚杨永安易铭章海燕唐希灿
Owner JIANGSU TIANSHENG PHARMA