Method for producing soy sauce by fermenting brewer grains
A technology of brewer's grains and soy sauce, which is applied in application, food preparation, food science, etc., can solve the problems of inconvenient long-distance transportation, long-term storage, and non-utilization, and achieve the effect of increasing added value, rich nutrition, and good flavor
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Embodiment 1
[0011] Cultivation of Aspergillus oryzae koji species: Weigh 38g of bran, 10g of soybean powder, 2g of glucose, and add 50mL of water to prepare a strain expansion medium, and inoculate it with Shanghai brewed rice that has been cultured and activated on bean sprout juice agar medium for 84 hours at a temperature of 28-30°C. 3.042, cultured in solid state at 28-30°C for 72 hours, if it passes the inspection, it is Aspergillus oryzae.
[0012] Cultivation method of Bacillus subtilis liquid seeds: Weigh 6g of soybean protein peptide powder, 4g of glucose, 0.1g of potassium dihydrogen phosphate, 0.05g of magnesium sulfate, add 90mL of water, stir well, sterilize at 121°C for 30 minutes, cool to 30°C, Insert the activated Bacillus subtilis slant seeds, stir evenly, and cultivate for 24 hours under the conditions of 28°C-30°C and a rotation speed of 180rpm, and if it passes the inspection, it is the Bacillus subtilis liquid seeds.
Embodiment 2
[0015] Cultivation of Aspergillus oryzae koji species: Weigh 72g of bran, 22g of soybean powder, 6g of glucose, and add 100mL of water to prepare a strain expansion medium, and inoculate the Hu Brew 3.042, incubate in solid state at 28-30°C for 72 hours, and if it passes the inspection, it is the Aspergillus oryzae koji species.
[0016] Cultivation method of Bacillus subtilis liquid seeds: Weigh 12g of soybean protein peptide powder, 8g of glucose, 0.2g of potassium dihydrogen phosphate, 0.1g of magnesium sulfate, add 180mL of water, stir well, sterilize at 121°C for 30 minutes, cool to 30°C, Insert the activated Bacillus subtilis slant seeds, stir evenly, and cultivate for 24 hours under the conditions of 28°C-30°C and a rotation speed of 180rpm, and if it passes the inspection, it is the Bacillus subtilis liquid seeds.
[0017] The specific implementation method of the present invention is as follows: Weigh 500 g of crushed low-temperature soybean meal, add 750 g of beer gr...
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