Preparation method of trehalose anhydrous

A technology of anhydrous trehalose and trehalose, which is applied in the preparation of sugar derivatives, separation methods, chemical instruments and methods, etc., can solve the problems of high cost, difficult to completely remove crystal water, difficult to promote, etc., to achieve complete dehydration, omit Crystallization step, steam saving effect

Active Publication Date: 2015-01-07
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no patent introduction on the production process of anhydrous trehalose. In the traditional drying process, fluidized bed is used for material dehydration. Generally, only free water can be removed, and crystal water is difficult to completely remove. High temperature equipment is used to remove crystal water, and the cost is high , it is difficult to promote in the industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method of anhydrous trehalose, comprising the steps of:

[0039] (1) Using maltose syrup with a concentration of 90% as raw material, after 24 hours of conversion by immobilized trehalose synthase, a reaction solution containing 50wt% trehalose was obtained; the pH of the reaction solution was controlled at 4.2-4.7, 58°C, and 15U of glucoamylase was added / mL, keep warm for 10h, then raise the temperature to 80°C, keep warm for 10min, and filter to obtain trehalose crude solution;

[0040] Add 1wt% activated carbon to the prepared trehalose crude enzyme solution, keep the temperature at 65°C for 15 minutes, filter and desalt the ion exchange system, control the flow rate of ion exchange to 500kg / h, and the temperature at 30°C to make the feed liquid clear Transparent, no smell, light transmittance ≥ 95%;

[0041] (2) After concentrating the decolorized trehalose crude liquid obtained in step (1) to a total sugar content of 50 wt%, a trehalose purified liq...

Embodiment 2

[0045] A preparation method of anhydrous trehalose, comprising the steps of:

[0046] (1) Using maltose syrup with a concentration of 90% as raw material, after 48 hours of conversion by immobilized trehalose synthase, a reaction solution containing 65wt% trehalose was obtained; the pH of the reaction solution was controlled at 4.2-4.7 and 62°C, and 30 U of glucoamylase was added / mL, keep warm for 15h, then raise the temperature to 100°C, keep warm for 30min, and filter to obtain trehalose crude solution;

[0047] Add 3wt% activated carbon to the prepared trehalose crude enzyme solution, keep the temperature at 100°C for 30 minutes, filter and desalt the ion exchange system, control the flow rate of ion exchange at 1000kg / h, and the temperature at 55°C to make the feed liquid clear Transparent, no smell, light transmittance ≥ 95%;

[0048] (2) After concentrating the decolorized trehalose crude liquid obtained in step (1) to a total sugar content of 60 wt%, a trehalose purif...

Embodiment 3

[0052] A preparation method of anhydrous trehalose, comprising the steps of:

[0053] (1) Using maltose syrup with a concentration of 90% as raw material, after 36 hours of conversion by immobilized trehalose synthase, a reaction solution containing 60wt% trehalose was obtained; the pH of the reaction solution was controlled at 4.2-4.7 and 60°C, and 25 U of glucoamylase was added / mL, keep warm for 12h, then raise the temperature to 90°C, keep warm for 20min, and filter to obtain trehalose crude solution;

[0054] Add 2wt% activated carbon to the prepared trehalose crude enzyme solution, keep the temperature at 80°C for 20 minutes, filter and desalt the ion exchange system, control the flow rate of ion exchange at 800kg / h, and the temperature at 40°C to make the feed liquid clear Transparent, no smell, light transmittance ≥ 95%;

[0055] (2) After concentrating the decolorized trehalose crude liquid obtained in step (1) to a sugar content of 60 wt%, enter the simulated moving...

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Abstract

The invention relates to a preparation method of trehalose anhydrous. The preparation method comprises the following step: by adopting a trehalose reaction solution prepared from high-concentration malt syrup by enzymatic conversion as a raw material, performing processes such as enzymolysis and impurity removal, activated carbon decolorization, ion exchange, simulated moving bed chromatographic separation and belt-type vacuum continuous drying and dehydration to prepare series products containing trehalose anhydrous with the purity of 50-99.5wt% so as to meet the special requirements of trehalose anhydrous as a drying agent in different fields of drying. A trehalose anhydrous production process disclosed by the invention is simple and low in cost, and can be used for saving energy and reducing consumption in a production process.

Description

technical field [0001] The invention relates to a method for preparing anhydrous trehalose, belonging to the technical field of functional sugar preparation. Background technique [0002] Trehalose is a safe non-reducing disaccharide that widely exists in nature, usually in the form of two molecules of crystal water, and its molecular formula is C 12 h 2 o 11 2H 2 O. Trehalose has no free aldehyde groups, is non-reducible, and is very stable to acid and heat. When it coexists with amino acids and proteins, it will not cause browning even if it is heated. Trehalose also has unique effects of preventing starch aging, preventing protein denaturation, and inhibiting lipid oxidation, etc., and can be used as an excellent food ingredient or food ingredient. At the same time, due to its non-reducing sugar characteristics composed of special disaccharide molecules, it can form a unique protective film on the cell surface under harsh conditions such as high temperature, high col...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C07H3/04C12P19/12B01D53/28
Inventor 刘宗利王乃强李克文胥九兵张莉张友亮肖兆玲
Owner BAOLINGBAO BIOLOGY
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