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Two-step cross-linking method for preparing keratin/peo bionanofibrous membranes

A technology of biological nanofibers and keratin fibers, applied in the direction of animal fibers, fiber types, fiber processing, etc., can solve the problems of high price of organic solvents, environmental pollution, and irritation of organic solvents.

Active Publication Date: 2016-08-17
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Xing Zhicai and others proposed a method for preparing keratin / PEO nanofibers using trifluoroethanol as a solvent (KeratinNanofibers as a Biomaterial, International Conference on Nanotechnology and Biosensors, IPCBEE Vol.2, 2011: 120-124), and obtained a relatively high keratin content. High keratin / PEO blended nanofibers, the keratin / PEO mixing ratio can reach 90:10, but the organic solvent used in the preparation of the spinning solution by this method is highly irritating, causing serious pollution to the human body and the environment. And the price of organic solvents is also very expensive
[0007] The above literature reports have reported on the keratin regeneration spinning technology from different aspects, but the spinning solution with water as the solvent has a high proportion of polymers in the spinning solution due to the low viscosity of the keratin solution, and the keratin The problem that it is difficult to increase the content in the spinning solution
When organic solvents are used as solvents, although the ratio of keratin in the spinning solution has increased to 90:10, there are problems of environmental pollution and high production costs in spinning with organic solvents.

Method used

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  • Two-step cross-linking method for preparing keratin/peo bionanofibrous membranes
  • Two-step cross-linking method for preparing keratin/peo bionanofibrous membranes
  • Two-step cross-linking method for preparing keratin/peo bionanofibrous membranes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] After the human hair fiber is opened, removed, cleaned and dried in sequence, it is cut into 3-6mm fiber segments, and immersed in a solution containing a reducing agent and a protein denaturant at a solid-to-liquid ratio of 2.5g / 250ml at 90 Stir at ℃ for 2h, the stirrer speed is 400r / min. The mixture obtained after stirring was filtered with an 80-mesh screen to remove unreacted fibers, and fine impurities in the filtrate were removed by centrifugation at a centrifugation speed of 3000 rpm for 1 h, and then the supernatant was taken. At room temperature, the supernatant is dialyzed with deionized water to remove small molecular substances in the solution, and then concentrated to obtain a pure keratin solution with a concentration of 5% by mass. The concentrated keratin solution is frozen for 24 hours with a freeze dryer at -40--60° C. and then dried for 24 hours to obtain keratin powder.

[0029] Prepare a keratin / PEO aqueous solution with a mass percentage concentra...

Embodiment 2

[0031] After the wool fiber is opened, impurity removed, washed and dried in sequence, it is cut into 3-6mm fiber segments, immersed in a solution containing a reducing agent and a protein denaturant at a solid-to-liquid ratio of 4g / 250ml and stirred at 85°C 2.5h, the speed of the stirrer is 350r / min. Filter the mixture obtained after stirring with a 100-mesh sieve to remove unreacted natural keratin fibers, and centrifuge to remove fine impurities in the filtrate at a centrifugal speed of 3500 rpm for 0.8 h, and then take the supernatant. At room temperature, the supernatant is dialyzed with deionized water to remove small molecular substances in the solution, and then concentrated to obtain a pure keratin solution with a concentration of 5.5% by mass. The concentrated keratin solution is frozen for 36 hours with a freeze dryer under the condition of -40° C. to 60° C. and then dried for 36 hours to obtain keratin powder.

[0032] Prepare a keratin / PEO aqueous solution with a...

Embodiment 3

[0034] After the rabbit hair fiber is opened, removed, cleaned and dried in sequence, it is cut into 3-6mm fiber segments, and immersed in a solution containing a reducing agent and a protein denaturant at a solid-to-liquid ratio of 5.5g / 250ml at 80 Stir at ℃ for 3h, the stirrer speed is 300r / min. Filter the mixture obtained after stirring with a 120-mesh sieve to remove unreacted natural keratin fibers, and centrifuge to remove fine impurities in the filtrate at a centrifugal speed of 4000rpm for 0.6h, and then take the supernatant. At room temperature, the supernatant is dialyzed with deionized water to remove small molecular substances in the solution, and then concentrated to obtain a pure keratin solution with a concentration of 6% by mass. The concentrated keratin solution is frozen with a freeze dryer at -40--60°C for 24 hours and then dried for 48 hours to obtain keratin powder.

[0035] Prepare a keratin / PEO aqueous solution with a mass percentage concentration of 10...

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Abstract

The invention provides a two-step crosslinking preparation method for a keratin / PEO biological nanofiber membrane. The two-step crosslinking preparation method for the keratin / PEO biological nanofiber membrane comprises blending keratin powder extracted from waste natural keratin filaments through a reduction method and PEO powder according to the mass ratio of 90 to 50 to 10 to 50, preparing keratin / PEO static spinning solution with the mass percent concentration of 7 to 15% with water serving as solvent, adding ethylene glycol diglycidyl ether crosslinking agent into the static spinning solution to perform initial crosslinking on the static spinning solution, improving the keratin spinnability, performing static spinning, hanging a keratin / PEO nanofiber membrane obtained through static spinning inside a closed container loaded with ethylene glycol diglycidyl ether solution under 25 to 80 DEG C to perform secondary steam crosslinking, or arranging the keratin / PEO nanofiber membrane into a closed container which is full of pure oxygen under normal temperature to perform oxidation crosslinking and obtaining the keratin / PEO biological nanofiber membrane which is insoluble in water.

Description

technical field [0001] The invention provides a two-step crosslinking method for preparing keratin / PEO biological nanofiber membranes, which belongs to the technical field of biological materials. Background technique [0002] Natural keratin fiber is a fiber protein composed of various amino acids. It mainly forms a highly cross-linked three-dimensional stable structure through disulfide bonds, hydrogen bonds, salt bonds and other bonds, and is insoluble under general conditions, hindering its wide application. In addition, natural keratin fibers exist in large quantities in nature, causing environmental pollution. Therefore, it is of great significance to regenerate waste keratin fibers. [0003] Due to the unique molecular structure of keratin fibers, it has biological characteristics such as excellent biocompatibility, low immunity and biodegradability, artificially purified keratin and chemically modified products have been used in materials, biology, medicine, etc. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/4382D04H1/728D01D5/00C08J3/24D06M13/11D06M11/34D06M101/12D06M101/30
CPCD04H1/4382D04H1/728D06M11/34D06M13/11D06M2101/12D06M2101/30
Inventor 范杰刘雍俞霞李佳雷通达
Owner TIANJIN POLYTECHNIC UNIV
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