Huangshan goldenrain tree tissue culture method

A technology of luan tree and tissue culture, applied in the biological field, can solve the problems of low reproduction coefficient of tillering method, no germination rate of sowing method, easy to be affected by seasons, etc., and achieve the effects of shortening cycle time, reducing dosage and saving cost.

Active Publication Date: 2015-01-14
济宁高新科达科技项目服务有限公司
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Problems solved by technology

[0003] The propagation of Huangshan eucalyptus can be carried out by sowing, tillering and cuttings, but the sowing method is prone to the problem of

Method used

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Embodiment 1

[0022] A method for tissue culture of Huangshan eucalyptus, characterized in that: comprising the following steps,

[0023] (1) Preparation of Huangshan eucalyptus leaf extract

[0024] Cut 50g of fresh eucalyptus leaves into small pieces smaller than 5mm×5mm, boil them in 500ml of distilled water for 2 hours, filter with glass fiber filter paper after cooling, and finally dilute the filtrate to 500ml to obtain the extract with a mass concentration of 10%. liquid;

[0025] (2) Primary induction culture

[0026] The primary induction medium is 3 / 4WPM+6-BA 0.5~1.0mg / L+NAA 0.05~0.08mg / L+2~3% extract+white sugar 20g / L+agar 5~8g / L, pH5.8; , 2~3% extracting solution refers to mass concentration, is formed by diluting the extracting solution that mass concentration is 10% in step (1);

[0027] Obtain the explants of the stem section of Eucalyptus huangshanense from the healthy and healthy plants of Eucalyptus huangshanensis, and inoculate the explants on the primary induction medi...

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Abstract

The invention relates to a Huangshan goldenrain tree tissue culture method. The Huangshan goldenrain tree tissue culture method comprises the following steps: (1) preparation of a Huangshan goldenrain tree leaf extracting solution; (2) primary induction culture: inoculating a Huangshan goldenrain tree stem explant on a primary induction culture medium for culture, wherein the primary induction culture medium is added with the Huangshan goldenrain tree leaf extracting solution; (3) secondary proliferation culture: inoculating a stem or a stem tip of a Huangshan goldenrain tree aseptic seedling obtained by the primary induction culture on a secondary proliferation culture medium in an aseptic manner for culture, wherein the secondary proliferation culture medium is added with the Huangshan goldenrain tree leaf extracting solution; (4) tissue culture seedling rooting: cutting a Huangshan goldenrain tree aseptic clump seedling obtained by the proliferation culture into individual plants and inoculating the individual plants on a rooting medium, wherein the rooting medium is added with the Huangshan goldenrain tree leaf extracting solution; (5) domestication and transplanting of test-tube seedlings. By adopting the scheme, the cycle time can be shortened by 15%, the cost can be saved by 20%, and the Huangshan goldenrain tree tissue culture method is applicable to factory production.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for tissue culture of Huangshan eucalyptus, belonging to the field of biotechnology. Background technique [0002] Huangshan eucalyptus (Koelreuteria integrifolia Merr.) is a deciduous tree belonging to Sapindaceae. It has become an ideal forest tree species for greening, beautification and soil and water conservation, and has rapidly developed into a landscape forest species in the Yangtze River Basin that replaces poplar and other common tree species. It is one of the top ten deciduous tree species for urban greening in my country. [0003] The propagation of Huangshan eucalyptus can be carried out by sowing, tillering and cutting, but the sowing method is prone to the problem of no germination or low germination rate, the tillering method has a low reproduction coefficient, and cutting seedlings are easily affected by seasons. Through the tissue culture of Huangsha...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈建中孙庆俊张海洋杨帆
Owner 济宁高新科达科技项目服务有限公司
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