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Application method of biological agent prepared from Lactobacillus acidophilus XD13R on reducing proportion of drug-resistant bacteria in pet body

A technology of Lactobacillus acidophilus and biological preparations, which is applied in the field of application of biological preparations prepared by Lactobacillus acidophilus XD13R to reduce the proportion of drug-resistant bacteria in pets, and can solve the problems of low health index, high poisoning index, and easy disease. Achieve the effect of eliminating sub-health state, reducing immunosuppression, and reducing poisoning index

Active Publication Date: 2015-01-21
HESHAN SEWETE BIOLOGICAL PROD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the survey data, the current sub-health situation of pets is very serious, their health index is getting lower and lower, their immunosuppression is getting worse, and their poisoning index is getting higher and higher. The cure after getting sick can read bigger and bigger

Method used

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  • Application method of biological agent prepared from Lactobacillus acidophilus XD13R on reducing proportion of drug-resistant bacteria in pet body
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  • Application method of biological agent prepared from Lactobacillus acidophilus XD13R on reducing proportion of drug-resistant bacteria in pet body

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Experimental program
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Effect test

Embodiment 1

[0026] Domestication and screening of Lactobacillus acidophilus strain XD13R: 4 strains of Bacillus (XD05B-XD08B), 3 strains of lactic acid bacteria (XD05R-XD07R), and 1 strain of yeast (XD05J) were purchased from the Culture Collection Center of Guangdong Institute of Microbiology. Guangzhou Baijia Supermarket purchased 2 strains of yeast (XD06J-XD07J), and then isolated and screened 8 strains of lactic acid bacteria (XD08-RXD15R) that were different from the above-mentioned strains in plaque shape and cell shape from nature, and collected a total of 4 strains Bacillus (XD05B-XD08B), 11 strains of lactic acid bacteria (XD05R-XD15R), and 3 strains of yeast (XD05J-XD07J), and then they were subjected to 10 generations of mutagenesis culture in pig manure environment with high proportion of drug-resistant bacteria, and finally selected A bacterial strain that can adapt to the above-mentioned pig manure water environment and can reduce the proportion of drug-resistant bacteria is ...

Embodiment 2

[0030] (1) Preparation of Lactobacillus acidophilus XD13R bacterial liquid: inoculate fresh culture of Lactobacillus acidophilus XD13R into 10~20 500ml Erlenmeyer flasks containing MRS medium, and cultivate in an incubator at 30°C After 48 hours, the XD13R bacterial liquid was obtained.

[0031] (2) Preparation of medium: with sucrose, peptone, yeast extract, KH 2 PO 4 , tomato juice and water are the main raw material preparation medium, and their weight percentages are:

[0032] Sucrose 4%, peptone 2.5%, yeast extract 1%, KH 2 PO 4 1%, tomato juice 4%, water balance.

[0033] (3) Take the above-mentioned Lactobacillus acidophilus XD13R bacterial liquid and inoculate it into the above-mentioned culture medium sterilized by high temperature, adjust the initial pH to 5~6.5, carry out anaerobic fermentation in the fermenter for 15 days, until the final pH is 3~6.5 4. Obtain the biological preparation prepared by the Lactobacillus acidophilus.

Embodiment 3

[0035] (1) Preparation of Lactobacillus acidophilus XD13R bacterial liquid: inoculate fresh culture of Lactobacillus acidophilus XD13R into 10~20 500ml Erlenmeyer flasks containing MRS medium, and cultivate in an incubator at 38°C After 40 hours, the XD13R bacterial liquid was obtained.

[0036] (2) Preparation of medium: with sucrose, peptone, yeast extract, KH 2 PO 4 , tomato juice and water are the main raw material preparation medium, and their weight percentages are:

[0037] Sucrose 4%, peptone 2.5%, yeast extract 1%, KH 2 PO 4 1%, tomato juice 4%, water balance.

[0038] (3) Take the above-mentioned Lactobacillus acidophilus XD13R bacterial liquid and inoculate it into the above-mentioned culture medium that has been sterilized at high temperature, adjust the initial pH to 5~6.5, and carry out anaerobic fermentation in the fermenter for 4 days, until the final pH is 3~6.5. 4. Obtain the biological preparation prepared by the Lactobacillus acidophilus.

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Abstract

The present invention discloses an application method of biological agent prepared from Lactobacillus acidophilus XD13R with a preservation number of CCTCCM2014216 on reducing proportion of drug-resistant bacteria in pet body. The method utilizes the Lactobacillus acidophilus strain XD13R to prepare a biological agent which reduces the proportion of drug-resistant bacteria in pet body, reduces the immunosuppression in pet body, lowers the poisoning index, and improves the health index.

Description

technical field [0001] The invention relates to an application method of a biological agent, in particular to an application method of a biological agent prepared by using Lactobacillus acidophilus XD13R capable of reducing the proportion of drug-resistant bacteria in animals to reduce the proportion of drug-resistant bacteria in pets. Background technique [0002] Pet breeding has become more and more popular, among which pet dogs and pet cats are the most popular. Taking pet dogs as an example, there are large dogs, medium dogs, and small dogs in terms of body shape, and Baume and so on in terms of breed. [0003] Pets come from wild animals. Since they were adopted by people, they have received all kinds of care from pet owners. They are completely different from wild animals in terms of living conditions, living environment, food structure, and activity methods. Life in middle cities is very different from wild conditions in terms of air quality, drinking water quality,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16
Inventor 杨雄
Owner HESHAN SEWETE BIOLOGICAL PROD
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