A method for preparing total flavonoids from Mongolian medicine blue potted flower

A technology of blue potted flower and total flavonoids, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of no research reports on the purification process of effective parts, achieve a simple and stable extraction and purification process, and promote effective utilization , the effect of reducing the clinical dosage

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A technology of blue potted flower and total flavonoids, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of no research reports on the purification process of effective parts, achieve a simple and stable extraction and purification process, and promote effective utilization , the effect of reducing the clinical dosage

CN104288189BActive Publication Date: 2018-01-12INNER MONGOLIA MEDICAL UNIV

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Embodiment 1

[0029] Example 1 Extraction process research of total flavonoids of Mongolian medicine blue potted flower

[0030] 1 Materials, instruments and reagents

[0031] 1.1 Materials

[0032] The medicinal material of blue potted flower was purchased from Inner Mongolia Medicinal Materials Company (origin: Xilin Gol) in March 2009, and it was identified as the dried inflorescence of Scabiosa tschilliensis Grunning, a plant of Dipsaceae, by Mr.

[0033] 1.2 Instruments

[0034] DZF-6050 vacuum drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd.); ES-J200-4 electronic balance (Shenyang Longteng Electronics Co., Ltd.); AB135-S electronic balance (METTLERTOLEDO); Guangming Medical Instrument Factory); TC-15 constant temperature heating mantle (Haining Huaxing Instrument Factory); RE-52CS-1 rotary evaporator (Shanghai Yarong Biochemical Instrument Equipment Co., Ltd.); TV-1901 double-beam UV-Vis spectrophotometer ( Beijing Puana General Instrument).

[0035] 1.3 Reagents ...

Embodiment 2

[0100] Example 2 Purification process research of total flavonoids of Mongolian medicine blue pot flower

[0101] 1 Materials, instruments and reagents

[0102] 1.1 Materials

[0103] The medicinal material of blue potted flower was purchased from Inner Mongolia Medicinal Materials Company in March 2009. It was identified as the dried inflorescence of Scabiosa tschilliensis Grunning, a plant of the family Dipsaceae by Mr. Qiao Juntang from the School of Pharmacy of Inner Mongolia Medical College; rutin reference substance, batch number 100080-200506 (National Institute for the Identification of Pharmaceutical and Biological Products);

[0104] S-8, D101, AB-8, HPD100, HPD400, X-5, D4020, D3520, NKA-9 type macroporous resin (Cangzhou Baoen Adsorption Material Co., Ltd.).

[0105] 1.2 Reagents

[0106] Sodium hydroxide (analytical pure), hydrochloric acid (analytical pure).

[0107] 1.3 Instruments

[0108] DZF-6050 vacuum drying oven (Shanghai Jinghong Experimental Equipme...

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Abstract

The invention discloses a method for extracting and purifying total flavone from Mongolian scabiosa. The method comprises the following steps: refluxing and extracting for 3 times by 16 times of ethanol of 70%, wherein each time lasts for 2.5h; controlling a weight ratio of crude drugs in sample liquid to dried resin at 1:2.1, a diameter-to-height ratio of a chromatographic column at 1:25, and a flow velocity at 4BV / h; after completely absorbing, eluting by de-ionized water until an elution is colorless, and eluting by 90% of ethanol 7BV, and controlling a flow velocity at 4BV / h; collecting the elution, and decompressing and condensing the elution until the elution is dried to obtain total flavone which is more than 28% in purity. The extracting and purifying process disclosed by the invention is simple, convenient and easy to implement, and lays a foundation for further research on active ingredients of the Mongolian scabiosa.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical engineering, and relates to a method for preparing total flavonoids from Mongolian medicine blue potted flowers. Background technique [0002] Mongolian blue potted flower comes from the dried inflorescences of Scabiosa comosa comosa Fisch.ExRoem.et Schult and North China blue potted flower Scabiosa tschilliensis Grunning. . Mainly produced in Inner Mongolia, Heilongjiang, Jilin, Liaoning, Hebei and other provinces. Sweet, astringent, blunt, dry, greasy, heavy, and cool in nature, it has the effect of clearing away heat and clearing "Xie Ri". Mongolian medicine is clinically used for diseases such as lung heat, liver heat, and throat heat. For hot cough, jaundice and other diseases, Mongolian medicine clinical prescriptions for hepatitis include blue potted flowers, such as Giwang-9, Eriligen-7, etc., and have good curative effects. Modern pharmacological studies have shown that the tota...

Claims

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Application Information

Patent Timeline

12 Jan 2018

Publication

CN104288189B

IPC: A61K36/185

CPC: A61K36/185; A61K2236/333; A61K2236/39; A61K2236/55

Inventors: 薛培凤; 赵子龙