Primer applicable to amplicon sequencing library construction, construction method, amplicon library and kit comprising amplicon library

A technology of amplicon library and amplification primer, which is applied in libraries, chemical libraries, nucleotide libraries, etc., can solve the problems of overestimating microbial diversity and low accuracy of sequencing data, and increase the number of mixed samples , Improve throughput and improve accuracy
CN104293783AInactive Publication Date: 2015-01-21天津诺禾致源生物信息科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
天津诺禾致源生物信息科技有限公司
Publication Date
2015-01-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a primer applicable to amplicon sequencing library construction, a construction method, an amplicon library and a kit comprising the amplicon library. The primer comprises a target fragment amplification primer sequence, a sequencing primer sequence and an adaptor sequence, wherein at least one end of a target fragment amplification primer sequence, obtained from the target fragment amplification primer sequence, the sequencing primer sequence and the adaptor sequence by virtue of an amplification step, in the amplicon library is provided with an internal label sequence; the internal label sequence is formed by using 6-10 basic groups in different permutation and combination patterns. The primer enables the constructed amplicon library to have the internal label, and therefore, the number of the mixed samples of the library can be increased and the flux of sequencing can be increased, and besides, contaminated data generated in the library construction process can be differentiated and removed and the accuracy of late data analysis can be improved; as a result, the abundance ratio of different floras in an original sample can be reflected more really.
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Description

technical field

[0001] The invention relates to the field of high-throughput sequencing, in particular, to a primer suitable for constructing an amplicon sequencing library, a construction method, an amplicon library and a kit containing the same. Background technique

[0002] Amplicon sequencing is the sequencing of PCR products or captured fragments of a specific length, mainly including 16S rDNA sequencing, 18S rDNA sequencing, ITS sequencing and functional gene detection. The sequence of a certain hypervariable region of 16S / 18S / ITS determined by the Illumina MiSeq second-generation high-throughput sequencing platform is used to reflect the differences between species in environmental samples in terms of bacterial, fungal, and archaeal classification, which is useful for the study of oceans and soils. The composition of microorganisms in environments such as , intestinal feces, etc. plays an important guiding role; similarly, more biological information can also be mined...

Claims

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